• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1995 Fiscal Year Final Research Report Summary

Evaluation of human sperm acrosome reastion by the activation of acroshin, and its clinical application

Research Project

Project/Area Number 06671690
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionTOKYO DENTAL COLLEGE

Principal Investigator

KANEKO Satoru  Tokyo Dental College, Lecturer, 歯学部, 講師 (40214457)

Project Period (FY) 1994 – 1995
KeywordsAcrosin / acrosome reaction / Concanavalin A / Cell affinity chromatography / Equilibrium sedimentation
Research Abstract

1. The acrosome reacted human sperm with progressive motility was purified by using cell affinity chromatography on Concanavalin A Sepharose.
2. The un-acrosome reasted sperm was separated by means of the continuous-step density gradient with an inner capiral, to avoid induction of the acrosome reaction, Percoll was made isotonic with Ca, NaHCO_3 free Hanks solution.
3. Hystochemical staining with FITC-Con A achieved to distinguish the acrosome reacted and un reacted sperm, the inner acrosome membrane exposed in consequence of the acrosome reaction had affinity against the agent.
4. Equilibrium sedimentation in Percoll revealed that the acrosome reacted sperm had lighter apparent density than those un-reacted.
5. Human sperm contained at least two arginine amidases. The above results, however, suggested it is difficult to monitor the acrosome reaction by activation of acrosin, which bound to the inner acrosome membrane.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Kuroda Y,Kaneko S,Matsuda Y,Akihama S,Nozawa S: "Selective isolation of acrosome reacted human sperm with progressive motiltiy by using cell affinity chromatography on Concanavalin A Sepharose" Androgia. 58. 7-12 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko S,Kuroda Y,Matsuda Y,Akihama S,Nozawa S: "Quantitative evaluation of human sperm acrosome reaction by using FITC-Concanavalin A" Human Rerod.(contributed).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaneko S,Kuroda Y,Matsuda Y,Akihama S,Nozawa S: "Equilibrium sedimentation of acrosome reacted and unreacted human sperm" Human Reprod.(contributed).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuroda Y,Kaneko S,Matsuda Y,Akihama S,Nozawa S: "Selective isolation of acrosome reacted human sperm with progressive motility by using cell affinity chromatography on Concanavalin A Sepharose" Abdrologia. 58. 7-12 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneko S,Kuroda Y,Matsuda Y,Akihama S,Nozawa S: "Quantitative evaluation of human sperm acrosome reaction by using FITC Concanavalin A" Human Reprod.(contributed).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaneko S,Kuroda Y,Matsuda Y,Akihama S,Nozawa S: "Equilibrium sedimentation of acrosome reacted human sperm." Human Reprod.(contributed).

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1997-03-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi