1996 Fiscal Year Annual Research Report

胎児仮死予防につながる妊娠ヒト子宮筋細胞内Ca排出機構についての研究

Research Project

Project/Area Number	06671698
Research Institution	Fukuoka University
Principal Investigator	瓦林達比古福岡大学, 医学部・産婦人科, 助教授 (30142350)
Keywords	子宮筋 / 単離細胞 / 細胞内Ca / オキシトシン / ベラパミル / ライアノジン / Fura-2
Research Abstract	妊娠ラット子宮縦走筋を採取して、細片化後にコラゲナーゼ処理をする事により縦走筋単離細胞を得た。得られた単離細胞にCa感受性蛍光指示薬Fura-2を負荷し、細胞内遊離Ca濃度変化を測定した。妊娠15、18、21日のラット子宮縦走筋細胞に200nMオキシトシンを作用させると、21》18>15日の順に強い収縮が得られた。オキシトシンは濃度依存性に細胞内遊離Caを増やすが、最大値に達するまでの時間は4〜5秒であった。そこで妊娠進行に伴う変化を検討すると、細胞内Ca増加速度も親和性も大きさも21》18>15日の順番になった。次に、オキシトシンによる細胞内遊離Ca増加作用のメカニズムを調べる為に、外液Ca除去、ベラパミル前投与、ライアノジン前投与により各々の差を比較した。その結果、外液Ca除去>ベラパミル前投与>ライアノジン前投与の順にオキシトシンの効果が抑制された。従って、オキシトシンの主な作用点は、表面膜の電位依存性and/or受容器作動性Caチャネルにあるが、一部は細胞内Ca貯蔵部位にもある事が証明された。

Research Products
(6 results)

All Other

All Publications (6 results)

[Publications] Lin,s-H.,Miyata,S.,Kawarabayashi.T.,Nakashima,T.,Kiyohara,T.: "Hypertrophy of oxytocinergic magnocellular neurons in the hypothalamic supraoptic nucleus from gestation to lactation." Zool.Sci.13. 161-165 (1996)
[Publications] Kaneko,Y.,Kawarabayashi,T.,Ikeda,M.,Sugimori,H.,Tsukamoto,T.: "Characteristics of rat uterus longitudinal muscle B-adrenergic receptors : Changes in Kinetic properties of the receptor during gestation." J.Mol Recog. 9. 233-238 (1996)
[Publications] Kawarabayashi,T.,Kobayashi,M.,Akahane,M.,Ajisawa,Y.: "Comparison of in vitro and in vivo inhibitory effects of peptide and nonpeptide oxytocin antagonists on radioligand binding and uterine contractility of rats during pregnancy." Am.J.Obstet.Gynecol.175. 1348-1355 (1996)
[Publications] Kawarabayashi,T.,Shojo,H.,Tsukamoto,T.: "Effects of oxytocin,prostaglandin F2α and prostaglandin Ee on intracellular free calcium coneentrations of longitudinal muscle cells isolated from term pregnant rat mycmetrium" Gynecol.Obstet.Invest.(in press). (1997)
[Publications] Kawarabayashi,T.,Shojo,H.,Nakamura,S.,Sugimori,H,.Tsukamoto,T.: "Changes in responsiveness of freshly isolated longitudinal muscle cells from rat uterus towards oxytocin during gestation : contractility and calcium signaling" Mol.Cell.Endocrinol.(in press). (1997)
[Publications] Nakao,K.,Inoue,Y.,Okabe,K.,Kawarabayashi,T.,Kitamura,K.: "Various effects of oxytocin on membrane excitability in pregnant human myometrium." Am.J.Obstet.Gynecol.(in press). (1997)

1996 Fiscal Year Annual Research Report

胎児仮死予防につながる妊娠ヒト子宮筋細胞内Ca排出機構についての研究

Principal Investigator

瓦林 達比古 福岡大学, 医学部・産婦人科, 助教授 (30142350)

Research Products

[Publications] Lin,s-H.,Miyata,S.,Kawarabayashi.T.,Nakashima,T.,Kiyohara,T.: "Hypertrophy of oxytocinergic magnocellular neurons in the hypothalamic supraoptic nucleus from gestation to lactation." Zool.Sci.13. 161-165 (1996)

[Publications] Kaneko,Y.,Kawarabayashi,T.,Ikeda,M.,Sugimori,H.,Tsukamoto,T.: "Characteristics of rat uterus longitudinal muscle B-adrenergic receptors : Changes in Kinetic properties of the receptor during gestation." J.Mol Recog. 9. 233-238 (1996)

[Publications] Kawarabayashi,T.,Kobayashi,M.,Akahane,M.,Ajisawa,Y.: "Comparison of in vitro and in vivo inhibitory effects of peptide and nonpeptide oxytocin antagonists on radioligand binding and uterine contractility of rats during pregnancy." Am.J.Obstet.Gynecol.175. 1348-1355 (1996)

[Publications] Kawarabayashi,T.,Shojo,H.,Tsukamoto,T.: "Effects of oxytocin,prostaglandin F2α and prostaglandin Ee on intracellular free calcium coneentrations of longitudinal muscle cells isolated from term pregnant rat mycmetrium" Gynecol.Obstet.Invest.(in press). (1997)

[Publications] Kawarabayashi,T.,Shojo,H.,Nakamura,S.,Sugimori,H,.Tsukamoto,T.: "Changes in responsiveness of freshly isolated longitudinal muscle cells from rat uterus towards oxytocin during gestation : contractility and calcium signaling" Mol.Cell.Endocrinol.(in press). (1997)

[Publications] Nakao,K.,Inoue,Y.,Okabe,K.,Kawarabayashi,T.,Kitamura,K.: "Various effects of oxytocin on membrane excitability in pregnant human myometrium." Am.J.Obstet.Gynecol.(in press). (1997)

瓦林達比古福岡大学, 医学部・産婦人科, 助教授 (30142350)