1995 Fiscal Year Final Research Report Summary
The effects of growth factors on synthesis of extracellular matrix by cells involved in proliferative vitreoretinopathy.
| Project/Area Number |
06671763
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Osaka University |
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Principal Investigator |
OHJI Masahito Osaka University, Medical School, Lecture, 医学部, 助手 (90252650)
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| Co-Investigator(Kenkyū-buntansha) |
TANO Yasuo Osaka University, Medical School, Professor, 医学部, 教授 (80093433)
WATANABE Hitoshi Osaka University, Medical School, Lecture, 医学部, 助手 (60252673)
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| Project Period (FY) |
1994 – 1995
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| Keywords | proliferative vitreoretinopathy / fibronectin / TGF beta / retinal glial cells / retinal pigment epithelial cells / retinoic acid |
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| Research Abstract |
The purpose of this project is to elucidate the relationship between proliferative vitreoretinopathy and the two kind of cells involved in proliferative vitreoretinopathy. The effect of transforming growth factor beta 2 (TGF beta 2) on fibronectin synthesis by cultured rat retinal pigmented epithelium and rat retinal glial cells was assessed. TGF beta 2 stimulated fibronectin synthesis by the two cells in a dose-dependent manner. Retinoic acid reduced TGF beta 2 induced-fibronectin synthesis by rat retinal glial cells.
We also developed a new wound healing model of retinal pigment epithelial cells in sheet culture. In this model, retinal pigment epithelial cells retains its characteristics including microvilli and tight junction better than the cells in the cell culture, producing a better model in wound healing.13EA03 : The effect of TGF beta on phagocytosis of rod outer segments by retinal pigment epithelial cells was also evaluated. TGF beta 2 reduced phagocytosis of rod outer segments by retinal pigment epithelial cells.
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