1995 Fiscal Year Final Research Report Summary
Midkine in retinal deqeneration of the rat
| Project/Area Number |
06671770
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Kagoshima University |
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Principal Investigator |
UNOKI Kazuhiko Kagoshima University, Medicine, University Hospital, Assistant Professor, 医学部・附属病院, 講師 (60193926)
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| Project Period (FY) |
1994 – 1995
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| Keywords | rat / mouse / midkine / bFGF / light damage / ischemia / rescue / retinal degeneration |
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| Research Abstract |
We evaluated the protective effects of midkine (MK), the product of a retinoic acid responsive gene, on constant-light induced retinal degeneration in albino Sprague-Dawley rats by anatomical and electrophysiological methods. After one week of constant light exposure, uninjected controls and those injected with phosphate buffer saline (PBS) lost most of the photoreceptor inner and outer segments and the thickness of the ONL was decreased. Eyes that were injected with MK or bFGF demonstrated a significant rescue in the photoreceptor layr with a 2-3 fold increase in the ONL thickness. ERGs were hardly detectable in uninjected eyes after 1 week of constant light. On the other hand, distinct responses were recordable in eyes which had an intravitreal injection with MK and bFGF,and the degree of ERG rescue in terms of the amplitude of b-wave was about 40 to 60% as compared with normal eyes. In the constant light-induced retinal damage of albino BALB/C adult mice, midkine provides a potential agent for the rescue from photoreceptor degeneration. bFGF did not show any effects in the light-damaged photoreceptor degeneration in mice.
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