• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1995 Fiscal Year Final Research Report Summary

Topoisomerase I inhibitor (CPT-11) sensitivity against neuroblastoma cells.

Research Project

Project/Area Number 06671786
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 小児外科
Research InstitutionHokkaido University

Principal Investigator

SASAKI Fumiaki  Hokkaido Univ., School of Med., Assistant professor, 医学部附属病院, 講師 (40178661)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Hiromasa  Hokkaido Univ., School of Med., Assistant Professor, 医学部, 助手 (30226881)
Project Period (FY) 1994 – 1995
KeywordsNeuroblastoma / Topoisomerase I inhibitor
Research Abstract

Aim : We would like to know the chemosensitivity of topoisomerase I inhibior (CPT-11) against neuroblastoma cells and then examine the factors relating the chemosensitivity such as toposimererase content in the cells and expressino of p-glycoprotein and GST-pi.
Materials and methods : As a topoisomerase In inhibitor CPT-11 (SN-38) and Topotecan provided by pharmaceutica companies were used. Neuroblastoma cell lines such as GOTO,TGW,SK-N-AS,SK-N-BE,SK-N-SH,IMR-32 were used. Chemosensitivity was evaluated with MTT assy and topoisomerase content were measures with indirect flowcytometric assy.
Results : 1) CPT-11 and topotecan were iffective in all neuroblastoma cell lines except SK-N-DZ.2) IC50 of CPT-11 and topotecan was correrated significantly. 3) P-glycoprotein was expressed immunohistochmically in 4/7 cell lines. In other 3 cell lines expressed faintly. No correlation between chemosensitiviy and p-glycoprotein expression was found. 4) GST-pi was expressed in allcell lines. There was no correlation and IC50 of CPT-11.6) Neuroblastoma cells contacted with CPT-11 accumulated to G2・M cell cycle stage on FCM,although content of topoisomerase II did not increase.
Conclusion : Topoisomerase I inhibitor (CPT-11) is effective against neuroblastoma cells. No factors relating chemosensitiviy, such as topoisomerase I inhibitor content and P-glycoprotein and GST-pi expression, was found.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 佐々木文章: "神経芽腫に対するトポイソメラーゼI阻害剤の抗腫瘍効果-薬剤体制因子との関連-" 小児がん. 31(1). 25-28 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐々木文章: "トポイソメラーゼI阻害剤の神経芽腫に対する効果-特にトポイソメラーゼ含量との関連-" 小児がん. 32(1). 17-21 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fumiaki Sasaki: "Chemosensitivity of topoisomerase I inhibitor to neuroblastoma cells." Jpn J of Pediatric Oncology. 31(1). 25-28 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fumiaki Sasaki: "Topoisomerase content in neuroblastoma cells and chemosensitivity of topoisometase I inhibitor." Jpn J of Pediatric Oncology. 32(1). 17-21 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1997-03-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi