1995 Fiscal Year Final Research Report Summary
Cytoskeletal study of rat osteoblast, employing in-situ hybridization
| Project/Area Number |
06671834
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | TOKYO DENTAL COLLEGE |
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Principal Investigator |
MORIGUCHI Mitsuko TOKYO DENTAL COLLEGE,Lecturer, 歯学部, 講師 (00085820)
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| Co-Investigator(Kenkyū-buntansha) |
YANAGISAWA Takaaki Tokyo Dental College, Proffessor, 歯学部, 教授 (10096513)
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| Project Period (FY) |
1994 – 1995
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| Keywords | In situ hybridization / Osteoblast / Beta-actin |
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| Research Abstract |
1. Actin-filament formation observed by the in-situ hybridization method
Beta-actin mRNA signals were observed by in-situ hybridization in osteoblasts in the calvaria periosteum but not in fibroblasts, presteoblasts included. this finding suggests that osteoblasts trigger actin-filament formation and maintain stress fibers because of the need for attachment to the bone surface.
2. Actin production and embryological detection
The only difference observed in periosteum beta-actin mRNA signals throughout all development stages was a decrease in the old-age phase of osteoblasts. This suggests that osteoblasts are constantly forming actin-filaments in all except the old stage.
3. Actin production and morphological changes in cells caused by administration of PGE_2
In the periosteum, 8 hours after of PGE_2 administration, beta-actin mRNA signals were stronger in fibroblasts, including preosteoblasts, than in osteoblasts. Such conditions result in alterations in fibroblast cell outlines, increases in actin filaments and stress fiber production.Therefore, the results of this study suggest that actin newly produced as a result of increased mRNA causes formation of stress fibers.
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