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1996 Fiscal Year Final Research Report Summary

ENHANCING EFFECTS OF EPIDERMAL GROWTH FACTOR ON HUMAN ORAL SQUAMOUS CELL CARCINOMA INVASION AND METASTASIS.

Research Project

Project/Area Number 06672015
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHEALTH SCIENCES UNIVERSITY OF HOKKAIDO

Principal Investigator

TOSHIYUKI Shibata  HEALTH SCIENCES UNIVERSITY OF HOKKAIDO SCHOOL OF DENTISTRY : ASSISTANT PROFESSOR, 歯学部, 講師 (50226172)

Co-Investigator(Kenkyū-buntansha) ARISUE Makoto  HEALTH SCIENCES UNIVERSITY OF HOKKAIDO SCHOOL OF DENTISTRY : PROFESSOR, 歯学部, 教授 (20091407)
Project Period (FY) 1994 – 1996
KeywordsHUMAN / SCC / ORAL CANCER / EGF / INVASION / MATASTASIS
Research Abstract

1) Effect of EGF in paracrine manner
In order to clear the effects of Epidermal Growth Factor (EGF) on the invasion abilities of human oral squamous cell carcinoma, five cell lines were studied. EGF enhanced the random motility of all cell lines in a dose-dependent fashion and exposure to EGF let to an increased production of urokinase type plasminogen activator and matrix metalloproteniase-9 by the same cells. These results strongly suggest the EGF may promote the invasion and metastasis of human oral squamous cell carcinomas. (Tumor Biology, 17 : 168-175,1996.)
2) EFFECT OF EGF IN AUTOCRINE MANNER
Cell lines, which produced EGF,was found out in these cell lines. In these cells, EGF produced by tumor cells can stimulate the random motility itself through the EGF receptor. (Oncologia 27 : 455-457,1994)
3) ESTABLISHMENT OF EGF SENSITIVE CLONE
To reveal the signal pathway, we established s-1 clone cells, which was most sensitive clone against the EGF stimulation and also we established i-1 clone cells, which was most insensitive clone. Comparison of these clone cells may lead the mechanisms of signal transduction of EGF-induced motility in near future.
4) ANALYSIS OF SIGNAL TRANSDUCTION OF EGF-INDUCED MOTILITY.
When EGF bind to the EGF receptor, tyrosine phosphorelation is firstly occurred and subsequently PKC is activated through the activation of PLCr arising from erbB homodimer and/or activation of PI3-kinase arising from erbB3 heterodimer. (Proceeding of JJCR,54th Annual Meeting, P458)

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 永易 裕樹: "上皮成長因子EGFが癌細胞の湿潤転移能に及ぼす影響…第1報" Jpn.J.Maxillofac.Surg.41. 682-689 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki SHIBATA: "Enhancing Effects of Epidermal Growth Factor on Human Squamous Cell Carcinoma Motility and Matrix Degradation but not Growth." Tumor Biology. 17. 168-175 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki SHIBATA: "Modulation of the Rat Tumor-associated Shedding Antigen(CE7) and Augumention of Immunogenicity by Irradiation." Anticancer Research. 16. 99-104 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永易 裕樹: "上皮成長因子EGFが癌細胞の浸潤転移能に及ぼす影響…第2報" Jpn.J.Maxillofac.Surg.42. 256-263 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki SHIBATA: "Enhancement of Tumor Antigen Expression and Inhibition of Pulmonary Metastasis of Rat fibrosarcoma Cells by Local Radiotherapy." Anticancer Research. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki SHIBATA: "Enhancement of Tumor Associated antigen Expression During the Regression Phase of Xemogenized Tumor Cell Growth in vivo." Anticancer Research. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takashi KAWANO,jun-ichi HAMADA,Hiroki NAGAYASU,Toshiyuki SHIBATA,Masuo HOSOKAWA,Noritoshi TAKEICHI: "EGF can stimulate the motility of human oral squamous cell carcinoma cells in autocrine manner" Oncologia. 27. 455-457 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroki NAGAYASU,Toshiyuki SHIBATA,Takashi KAWANO,Akira KONISHI,Kazuhiko OKUMURA,Makoto ARISUE: "Effects of epidermal growth factor (EGF) on the invasive and metastatic potential of tumor cells : Part 1, Possibility of malignant phenotype aquisition by long-term EGF treatment" Jpn.J.Maxillofac.Surg.41. 682-689 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki SHIBATA,Takashi KAWANO,Hiroki NAGAYASU,Kazuhiko OKUMURA,Makoto ARISUE,Jun-ichi HAMADA,Noritoshi TAKEICHI,Masuo HOSOKAWA: "Enhancing Effects of Epidermal Growth Factor on Human Squamous Cell Carcinoma Motility and Matrix Degradation but not Growth." Tumor Biology. 17. 168-175 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki SHIBATA,Masuo HOSOKAWA,Mark MICALLEF,Takanori MIZUKOSHI,Ren JIN,Itsuo CHIBA,Noritoshi TAKEICHI,Makoto ARISUE: "Modulation of the Rat Tumor-associated Shedding Antigen (CE7) and Augumention of Immunogenicity by Irradiation." Anticancer Research. 16. 99-104 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroki NAGAYASU,Toshiyuki SHIBATA,Takashi KAWANO,Akira KONISHI,Kazuhiko OKUMURA,Makoto ARISUE: "Effects of epidermal growth factor (EGF) on the invasive and metastatic potential of tumor cells : Part 2 : Mechanisms of malignant phenotype acquistion by long-term EGF treatment" Jpn.J.Maxillofac.Surg.42. 256-263 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki SHIBATA,Masuo HOSOKAWA,Mark MICALLEF,Itsuo CHIBA,Takanori MIZUKOSHI,Motoyasu KATOH,Noritoshi TAKEICHI,Hiroshi KOBAYASHI: "Enhancement of Tumor Antigen Expression and Inhibition of Pulmonary Metastasis of Rat fibrosarcoma Cells by Local Radiotherapy." Anticancer Research. 17 : (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki SHIBATA,Masuo HOSOKAWA,Mark MICALLEF,Itsuo CHIBA,Noritoshi TAKEICHI,Hiroshi KOBAYASHI: "Enhancement of Tumor Associated antigen Expression During the Regression Phase of Xenogenized Tumor Cell Growth in vivo." Anticancer Research. 17 : (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroki NAGAYASU,Jun-ichi HAMADA,Takashi KAWANO,Shigeo KONAKA,Daichi NAKATA,Toshiyuki SHIBATA,Makoto ARISUE,Masuo HOSOKAWA,Noritoshi TAKEICHI,Tetsuya MORIUCHI: "Inhibitory Effects of Malotilate on Invasion and Metastasis of Rat Mammary Carcinoma Cells by Modifying the Functions of Vascular Endotherial Cells." British Journal of Cancer. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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