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1995 Fiscal Year Final Research Report Summary

The function of vascular endothelial cells and roles of integrin and extracellular matrix

Research Project

Project/Area Number 06672226
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionTokyo Metropolitan Institute of Gerontology

Principal Investigator

YAMAMOTO Kiyotaka  Tokyo Metro.Inst.Geront.Dept.Cell Biol.Senior Researcher, 細胞生物, 主任研究員 (90073022)

Project Period (FY) 1994 – 1995
KeywordsHuman endothelial cells / Integrin / Cytoskeleton / Extracellular matrix / Collagens / Cell adhesion / Smooth muscle cells / Signal transduction
Research Abstract

1.We examined the F-actin filaments and the expression of integrin in human vascular endothelial cells cultured on type I and type V collagens.The cells attached to type V collagen, formed complete F-actin filaments, and expressed alpha2, alpha3, and beta1 integrins 0.5-1 h after inoculation.These phenomena are referred to as the first ECM-integrin-cytoskeleton system.After 24h, the cells on type V collagen failed to form the second system, and gradually detached from the substrate.In contrast, the cells on type I collagen developed both the first and second systems, and acclimatized themselves to the environment.The cell detachment from type V collagen is not attributed to endogenously produced TSP with anti-adhesive properties, but resulted from the failure to reconstitute the second ECM-integrin-cytoskeleton system in focal adhesion.
We next attempted to identify the cell surface integrins for native and heat-denatured type I collagens in primary and secondary cultures of rabbit aort … More ic smooth muscle cells (SMC).The cells in the contractile state adhered to native type I collagen through the alpha1beta1 and alpha1beta1 integrins, but the relative amount of alpha3 integrin decreased.The initial adhesion of cells in secondary culture to native type I collagen was mediated only by the alpha1beta1 and alpha3beta1 integrin.The cell-binding sequences did not contain DGEA or RGD.In contrast, cell adhesion to denatured type I collagen was mediated only by the alpha1beta1 integrin in the contractile state and by the alpha1beta1、alpha2beta1, and alpha3beta1 integrins in the synthetic state.In denatured type I collagen, the sequences DGEA and RGD served as a recognition site for the alpha2beta1 integrins.Our results suggest that rabbit SMC can recognize the native and denatured type I collagens throgh interactions with triple helix-binding receptors and alpha3 chain-binding receptors and that the expression pattem of integrins changes in conjunction with the phenotypic properties of vascular SMC. Less

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] K. Yamamoto: "Changes in elastin-binding proteins during the phenotypic transition of rabbit arterial smooth muscle cells in primary culture" Exp. Cell Res.218. 339-345 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Yamamoto: "Identification of integrins involved in cell adhesion to native and denatured type I collagens and the phenotypic transition of rabbit arterial smooth muscle cells" Exp. Cell Res.219. 249-256 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Yamamoto: "The differentiated properties of vascular smooth muscle cells and the roles of extracellular matrix" Connetive Tissue. 26. 327-334 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N. Hasegawa: "Elvated promotion of prostacyclin production by synthetic lipid A analogs in aged human endothelial cells in culture" Mech. Ageing Dev.78. 155-162 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Aoyage: "Kinetics of ^<125>I-PDGF binding and down-regulation of PDGF receptor in human arterial smooth muscle cell strains during cellular senescence in vitro" J. Cell. Physiol.219. 376-384 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Yamamoto: "The failure to reconstitute the second extracellular matrix-integrin-cytoskelton system in human endothelial cells on type V collagen" Artery. 21. 76-93 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Yamamoto: "Growth regulation of rabbit arterial smooth muscle cells by platelet-derived growth factor, insulin-like growth factor-I,and epidermal growth factor in primary culture.Exp.Cell Res., 212 : 1,62-68." Exp.Cell Res.212. 62-68 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Fukai: "Human arterial smooth muscle cell strains derived from patients with moyamoya disease : changes in biological characteristics and proliferative response during cellular aging in vitro.19GC02 : Mech.Ageing Dev." 75. 21-33 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamamoto: "Cell adhesion receptors for native and denatured type I collagens in rabbit arterial smooth muscle cells in culture." Exp.Cell Res.214. 258-263 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamamoto: "Role of elastin in differentiated properties of rabbit arterial smooth mscle cells in culture." Connective Tissue. 26. 245-254 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamamoto: "The failure to reconstitute the second extracellular matrix-integrin-cytoskeleton system in human endothelial cells on type V collagen." Artery. 21. 76-93 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Hasegawa: "Elevated promotion of prostacyclin production by synthetic lipid A analogs in aged human endothelial cells in culture." Mech.Ageing Dev. 78. 155-162 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamamoto: "The differentiated properties of vascular smooth muscle cells and roles of roles of extracellular matrix." Connective Tissue. 26. 327-334 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamamoto: "Changes in elastin binding proteins during the phenotypic transition of rebbit arterial smooth muscle cells in primary culture." Exp.Cell Res.218. 339-345 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Aoyagi: "Kinetics of 125I-PDGF binding and down-regulation of PDGF receptor in human arterial smooth muscle cell strains during cellular senescence in vitro." J.Cell.Physiol.164 : 2. 376-384 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Yamamoto: "idetification of integrins for native and denatured type I collagens in the phenotypic transition of rabbit arterial smooth muscle cells." Exp.Cell Res.219. 249-256 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Aoyagi: "Immunohistochemical detection of Ki-67 in replicative smooth muscle cells of rabbit carotid arteries after balloon denudation." Stroke. 26. 2328-2332 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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