1995 Fiscal Year Final Research Report Summary
Effects of cytokine network on tumor metastasis and progression of chemokine gene transfected cells in vivo
| Project/Area Number |
06672270
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用薬理学・医療系薬学
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| Research Institution | Kanazawa University |
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Principal Investigator |
NAKASHIMA Emi KANAZAWA UNIVERSITY HOSPITAL PHARMACY ASSOCIATE PROFESSOR, 医学部・附属病院・薬剤部, 助教授 (90115254)
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| Co-Investigator(Kenkyū-buntansha) |
ICHIMURA Fujio KANAZAWA UNIVERSITY HOSPITAL PHARMACY PROFESSOR, 医学部・附属病院・薬剤部, 教授 (40143911)
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| Project Period (FY) |
1994 – 1995
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| Keywords | cytokine / gene transfer / metastasis / tumor immunology |
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| Research Abstract |
Effects of chemokine on tumor progression and metastasis were studied. Cachexia-inducing adenocarcinoma cell line cells (colon 26, clone 20) were transfected with either a control plasmid or chemokine (MCAF) cDNA.The production of MCAF reached 1.9 ng/ml in vitro when transfectant cells were cultured at a cell density of 5*10^4 cells/ml for 3 days. Transfection of MCAF cDNA did not affect the growth rate in vitro. Also, MCAF-transfectants formed a similar size of tumors and induced the same degree of cachexia after intra-footpad inoculation as the parental cells. When the primary tumors were resected on the 10th day after inoculation, the incidence rate of spontaneous lung metastasis was less than 20% in both transfectant and parental cells. The number of endothelial cells in the primary tumor rapidly increased from the 10th to the 14th day after inoculation. In accordance with enhanced angiogenesis, the incidence rates of spontaneous metastasis increased when the primary tumors were resected on the 14th day after inoculation. Moreover, spontaneous lung metastases as well as experimental lung metastases were augmented in the animals injected with MCAF-transfectants compared to those injected with parental cells with a concomitant increase of angiogenesis. These results suggested that MCAF may augment the metastatic potential by modulating tumor associated angiogenesis.
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