1995 Fiscal Year Final Research Report Summary
Study on structural heterogeneity of Lp(a) and its clinical significance.
| Project/Area Number |
06672289
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Gifu University |
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Principal Investigator |
MAKINO Kazuhiko Gifu University School of Medicine, Assistant, 医学部, 助手 (80181618)
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| Co-Investigator(Kenkyū-buntansha) |
NOMA Akio Gifu University School of Medicine, Professor, 医学部, 教授 (30208384)
ABE Akira Gifu College of Medical Technology, Professor, 教授 (30175898)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Lp(a) lipoprotein / Apolipoprotein(a) / structural heterogeneity / Phenotype / Genotype / Allele number |
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| Research Abstract |
The cDNA sequence of apo(a), specific apolipoprotein of Lp(a) lipoprotein, has revealed a high degree of homology with plasminogen. The differences in size of apo(a) isoforms are due to allelic differences in the number of kringle IV-like units.
In the present study, a new sensitive system has been employed to determine the apo(a) phenotypes using the commercial Lp(a) phenotype analyzing kits for separation and the western-blotting for detection. Furthermore, we have investigated the basis of the apo(a) size polymorphism by pulsed field gelelectrophoresis of genomic DNA employing KpnI restriction enzyme and an apo(a) kringle IV-specific probe.
No null type was observed in the two sensitive methods, and frequencies of detectable double-bands were 79.5% in phenotyping and 88.6% in genotyping. The frequencies of allele numbers and the biphasic histograms for allel numbers were similar in these two metods.
In conclusion, we are able to obtain many informations for Lp(a) genetics by use of the present sensitive phenotyping system.
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