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1995 Fiscal Year Final Research Report Summary

Secretion and regulation of endothelin and

Research Project

Project/Area Number 06672297
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionFukushima Medical Collge

Principal Investigator

YOSHIDA Hiroshi  Dept.Clin.Lab.Med.Fukushima Medical College Prof, 医学部・臨床検査医学, 教授 (90045683)

Co-Investigator(Kenkyū-buntansha) OHARA Morihiro  Dept.Clin.Lab.Med.Fukushima Medical College assist prof, 医学部・第二内科, 講師 (60128543)
NAGAI Toshihiko  Dept.Clin.Lab.Med.Fukushima Medical College associate, 医学部, 助手 (90180447)
Project Period (FY) 1994 – 1995
Keywordsendothelin / ALP / synoviocyte / type Acell / joint inflammation / 関節病変
Research Abstract

Macrophge-like type A like synoviocytes from RA patients were shown to secrete endothelin (ET), alkaline phosphatase (ALP) and lactic dehydrogenase (LDH). The mechanism andproperties of these substances were investigated.
ET-1 was secreted from macrophage-like synoviocytes, but not from fibroblast-like B cells. Analysis by HPLC disclosed that ET-1 was the major ET (CCA,submitted).
Furthermore, cultures fluids of these synoviocytes were screened for about 30 items and higher levels of ALP and LDH were detected.
ALP secreted from type A like cells distributed in alpha-beta region on electrophoresis. Properties are as follows : Neuraminidase sensitive, heat-stable (56゚C,10-30 min), slight inhibition by 1-phenylalanine and high affinity to wheat germ lectin (90%).
Synovial fluids of RA and OA patients were examined for the heat-stable ALP.Some RA synovial fluids contained more than 10% of the ALP.
The significances of ET and ALP in the pathogenesis and participation in the joint inflammations is unknown.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Kasai S, et al: "Peraiterncho absovgtion of indomethacin…" Jap J. Rheumatol. 5. 199-206 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kasai S. et al: "Absovption of indomctacin from skin of human" J. Pharmacent. Sa. (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida, H et al: "Heat stable ALP seorcted from synovioafle" XVI ICCC(London). (発表予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida, H et al: "Imflueuce of ranitidinc on pharmdco kinctics of …" Asia Pacific LAR(メルボルン). (発表予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ozawa, Y. et al: "Effect of temperaturc and drugs on potassion" Quality Control in the Clinical Laboratory. 191-196 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagai, T. et al: "Cold activation of complement and seuem stovege" QC in the clivical Laboratory. 163-169 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉田浩: "臨床検査のABC(分担)" 日本医師会, 5 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉田浩: "Laboratory Medicine(分担)" 医歯薬出版, 14 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida, H.et al: "Production of endothelin-1 by cultured human synoviocytes" CCA. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, H.et al: "ALP secretio from synovial cells of RA patients" 1995 ALP criference.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, H.et al: "Heat stable ACP secreted from synoviocytes of gatients with RA" 16 th IFCC. (**** 1996.7.London).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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