• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1995 Fiscal Year Final Research Report Summary

Studies on Processing Endoproteases of Biologically Active Peptide Precursors

Research Project

Project/Area Number 06680604
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionUniversity of Tsukuba

Principal Investigator

NAKAYAMA Kazuhisa  Univ.of Tsukuba, Inst.of Biological Science Associate Prof., 生物科学系, 助教授 (40192679)

Project Period (FY) 1994 – 1995
KeywordsProcessing endoprotease / Kex2 protease / furin / paired basic amino acids
Research Abstract

In endocrine and neuronal cells, many biologically active peptides are synthesized as larger precursors, which undergo limited endoproteolysis at paired basic amino acids to yield bioactive products. Recently, six endoproteases (furin, PC2, PC3, PC4, PACE4, and PC6) has been shown to be involved in this type of processing. In this study, we have shown the followings with respect to furin.
1. We have discovered a mammalian cell line that lacks endoproteolytic activity due to mutations of furin. We have shown using this cell line that furin is the most important protease for processing of precursors of many proteins.
2. Furin is mainly localized to the trans-Golgi network (TGN) within cells. We have shown that, for the TGN-localization of furin, its cytoplasmic domain is essential, and Ser and Tyr residues within this domain play an important role.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Senye Takahashi: "A second mutant allele of furin in the processing-incompetent cell line. LoVo." J. Biol. Chem.270. 26565-26569 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Senye Takahashi: "Localization of furin to the trans-Golgi network and recycling from the cell surface involves Ser and Tyr residues within the cytoplasmic domain." J. Biol. Chem.270. 28397-28401 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Senye Takahashi: "A second mutant allele of furin in the processing-incompetent cell line, LoVo" J.Biol.Chem.270. 26565-26569 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Senye Takahashi: "Localization of furin to the trans-Golgi network and recycling from the cell surface involves Ser and Tyr residues within the cytoplasmic domain" J.Biol.Chem.270. 28397-28401 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1997-03-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi