1995 Fiscal Year Final Research Report Summary

STUDY OF TRANSCRIPTIONAL REGULATION MEDIATED BY INOSITOL-SIGNAL TRANSDUCTION

Research Project

Project/Area Number	06680670
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Molecular biology
Research Institution	KYUSHU INSTITUTE OF TECHNOLOGY
Principal Investigator	NIKAWA Junichi KYUSHU INSTITUTE OF TECHNOLOGY.FACULTY OF COMPUTER SCIENCE AND SYSTEMS ENGINEERING,ASSOCIATE PROFESSOR, 情報工学部, 助教授 (00134271)
Project Period (FY)	1994 – 1995
Keywords	Inositol / Signal transduction / Transcriptional regulation / Human cDNA
Research Abstract	The Saccharomyces cerevisiae ire15 mutant has a defect in the expression of INO1, showing an inositol auxotrophic phenotype. We isolated and characterized the yeast SCS2 gene and human HCP1 gene, both can suppress the growth defect of yeast ire15 mutant. Sequence analysis revealed that SCS2 and human HCP1 encode 244 and 360 amino acids, respectively. Both gene products are highly conserved among species. Yeast homolog of HCP1 was isolated and tested for its ability to suppress the ire15 mutation. The results show that the yeast HCP1 also suppress the ire15 mutation. Northern blot analysis revealed that the introduction of these genes into ire15 mutant clearly restored the expression of INO1. These results indicate that these gene products are involved in the regulation of INO1 expression.