1995 Fiscal Year Final Research Report Summary
Determination of signal transduction pathways involving Ash/Grb2, the adaptor molecule composed of the Src homology regions
| Project/Area Number |
06680706
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Tokyo Metropolitan Institute of Gerontology |
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Principal Investigator |
MATUOKA Koozi Tokyo Metropolitan Institute of Gerontology Department of Biosignal Research Research Scientist, 生体情報部門, 研究員 (60110029)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Signal transduction / Src homology / Ash / Grb2 / SH2 / SH3 / Ras / Small GTP-binding protein |
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| Research Abstract |
We and another group recently discovered an adaptor protein Grb2/Ash.Here we investigated intramolecular interactions involving Ash to find the following : Ash plays a role in the insulin induction of membrane ruffling ; By its SH2 domain Ash binds directly to the EGF receptor but not to the NGF receptor upon stimulation ; Ash exhibits differential binding to the phosphorylated tyrosines of the EGF receptor (directly to Y 1068 and Y 1086 and indirectly to tyrosine 1148 via Shc) ; Ash binds by its SH3 domains to a variety of signaling molecules, including dynamin, C3G,N-WASP and synaptojanin ; a cDNA library screening resulted in discovery of splicing isomers of Ash, those exhibiting a different binding specificity to the above Ash target molecules.The results show that Ash is a multifunctional signal transducer down from the receptor protein tyrosine kinases.
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