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1996 Fiscal Year Final Research Report Summary

The significance of phosphorylation of neurofilament protein

Research Project

Project/Area Number 06680738
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionOsaka University

Principal Investigator

GOTOW Takahiro  Osaka University Medical School, Lecturer, 医学部, 講師 (20135693)

Co-Investigator(Kenkyū-buntansha) TAKEDA Masatoshi  Osaka University Medical School, Professor, 医学部, 教授 (00179649)
Project Period (FY) 1994 – 1996
KeywordsNeurofilament / NF-H / phosphorylation / axon / node of Ranvier / crossbridge / myelin sheath / nonphosphorylation
Research Abstract

In neurons, neurofilaments (NF) are relatively fewer in somata and dendrites but abundant in axons. In addition to this NFpolarity, the NF subunit protein, especially NF-H,is extremely phosphorylated in axons, and much less phosphorylated in somata and dendrites. NF-H is essential for forming crossbridges between core filaments, and we are thinking that its phosphorylation seems necessary to form well-developed crossbridges. To confirm our idea, we have obtained five main results on the relationship between NF-H phosphorylation and NF organization during these three years. 1).Both core filaments and crossbridges were regular in arrangement and NF-H was well phosphorylated in thicker myelinated axons, whereas they were less regular and less phosphorylated in thinner axons where microtubules were conspicuous. 2).In the node of Ranvier, where axon becomes smaller in diameter, core filametns were closer to each other and crossbridges were less regular and NF-H wa less phosphorylated than t … More he remainder of the same axon. 3).After microinjection of NF-H protein into cultured nonneuronal or neuron-like cells, NF-H was incorported into intermediate filaments and tended to make bundles of the filaments with rather higher frequency in crossbridges when it was phosphorylated. 4).In vitro experiments showed that NF could interact with microtubules or mitochondria through the crossbridges formed by NF-H,although we are now examining which condition in phosphorylation of NF-H is more efficient for such interactions. 5).At present, we are clarifying the relationship between the myelination and NF organization or NF-H phosphorylation with jimpy mutanat mice, where the myelinogenesis is distrupted in the central nervous system. It has just been found that nonphosphorylated form of NF-H increases drastically and core filaments are much more compact in the myelin-deficient axons than in controls. In conclusion, our data indicate that there is an intimate relationship between NF-H phosphorylation and NF organization, and also that it is necessary for the axon to have regular arrangements in crossbridges and finally in core filaments by NF-H phosphorylation. Furthermore, it is considered that the myelination increases in NF-H phosphorylation level resulting in normal NF oganization in axons. Less

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Gotow, T.: "Phosphorylation of neurofilament H subunit as related to arrangement of neurofilaments" Journal of Neuroscience Research. 37. 691-713 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gotow, T.: "Dephosphorylation of the largest neurofilament subunit protein influences the structure of crossbridges in reassemble neurofilaments" Journal of Cell Science. 107. 1949-1957 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gotow, T.: "The organization of neurofilaments accumulated in perikaryon following aluminum infoxication : relationship between structure and phosphorylation of neurofilaments" Neuroscience. 64. 553-569 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gotow, T.: "Dynamic structure and function of neurofilaments" Annals of Psychiatry. 5. 91-111 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeda, M.: "Neurofilament pathology in neurodegenerative processes of Alzheimer's disease" Annals of Psychiatry. 5. 113-126 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gotow, T.: "Preferential localization of annexin V to the axon terminal" Neuroscience. 75. 507-521 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gotow, T.and Tanaka, J.: "Phosphorylation of neurofilament H subunit as related to arrangement of neurofilaments" Journal of Neuroscience Research. 37. 691-713 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gotow, T., Tanaka, T., Nakamura, Y.and Takeda, M.: "Dephosphorylation of neurofilament H subunit protein influences the structure of crossbridges in reassembled neurofilaments" Journal of Cell Science. 107. 1949-1957 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gotow, T.and Tanaka, J.: "The organization of neurofilaments accumulated in perikaryon following aluminum intoxication : relationship between structure and phosphorylation of neurofilaments" Neuroscience. 64. 553-569 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gotow, T.: "Dyanamic structure and function of neurofilaments" Annals of Psychiatry. 5. 91-111 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeda, M., Gotow, T.and Nishimura, T.: "Neurofilament pathology in neurodegenerative processes of Alzheimer's disease" Annals of Psychiatry. 5. 113-126 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gotow, T., Sakata, M., Funakoshi, T.and Uchiyama, Y.: "Preferential localization of annexin V to the axon terminal" Neuroscience. 75. 507-521 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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