1995 Fiscal Year Final Research Report Summary
MORPHOLOGICAL STUDY ON THE SURVIVAL AND AXONAL GROWTH OF TELENCEPHALIC AND BRAINSTEM NEURONS BY S-100beta.
| Project/Area Number |
06680744
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | DOKKYO UNIVERSITY (1995)
Kyoto Prefectural University of Medicine (1994) |
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Principal Investigator |
UEDA Shuichi DOKKYO UNIVERSITY,SCHOOL OF MEDICINE,PROFESSOR, 医学部, 教授 (60150570)
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Noriyaki KYOTO PREFECTURAL UNIVERSITY,OF MEDICINE,RESEARCH ASSOCIATE, 医学部, 助手 (50239662)
AIKAWA Masuo DOKKYO UNIVERSITY,SCHOOL OF MEDICINE,RESEARCH ASSOCIATE, 医学部, 助手 (00049256)
OKA Atsuko DOKKYO UNIVERSITY,SCHOOL OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (50175254)
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| Project Period (FY) |
1994 – 1995
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| Keywords | S-100beta / Serotonin / Mutant mouse / Glia / Immunohistochemistry / Growth factor / Raphe nucleus / Hippocampus |
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| Research Abstract |
The homozygote of a mouse strain with genetic polydactyly (Polydactyly Nagoya ; Pdn) shows significant decrease of S-100beta in the brain. in order to clarify the effects of the retarded production of S-100beta on the development of monoaminergic neuron systems and glia cells, immunohistochemical studies of tyrosine hydroxylase (TH), serotonin, S-100beta and glial fibrillary acidic protein (GFAP) was performed. In all types of mice, S-100beta positive cells and serotonergic fibers were widely distributed throughout the brains and serotonergic cell bodies were located in the brainstem. However, the hippocampus and caudo-dorsal cortex of Pdn/Pdn mouse were markedly reduced in S-100beta positive cells and in serotonergic fibers. Furthermore, abnormal distribution of GFAP positive cells and fibers were observed in the cortex and hippocampus of Pdn/Pdn brain. No differences were seen in the distribution of TH neurons and fibers distribution. The present result suggests that the developmenta
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l defect of serotonergic fibers in the Pdn mutant mouse is correlate to the deficiency of S-100beta in the astrocyte of this mutant. To further clarify the effects of target tissues from different type brains on the development od serotonergic neurons, raphe neurons from Pdn/Pdn or+/+ newborn mice were co-cultured with hippocampus or cortex of +/+ or Pdn/Pdn newborn mice. The growth of serotonergic neurons in the mesencephalic raphe tissue dissociated cultures was estimated by measuring the specific uptake of [^3H] 5HT.The development of both genotypes (Pdn/Pdn and +/+) of serotonergic neurons was enhanced by co-cultures with target tissue (hippocampus and cortex) of +/+ brain. This effect was not observed in the co-cultures with Pdn/Pdn brain as a source of target tissue. The present results support the idea that the developmental defect of serotonergic fibers in the Pdn mutant mouse is correlate to the deficiency of S-100beta in the astrocyte of this mutant, and suggest that S-100beta is a serotonergic growth factor. Less
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[Publications] Ueda, S., Matsumoto, Y., Ishizuya-Oka, A., Aikawa, M., Nishimura, A.and Kawata, M.: "Organization of regenerating serotonergic fibers in the hippocampal formation." Brain Res.Bull. 39. 89-99 (1996)
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