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1995 Fiscal Year Final Research Report Summary

The role of cytokines in autoimmune encephalomyelitis and rejection processes of neural grafts

Research Project

Project/Area Number 06680751
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Nerve anatomy/Neuropathology
Research InstitutionTokyo Metropolitan Institute for Neuroscience

Principal Investigator

MATSUMOTO Yoh  Tokyo Metropolitan Institute for Neuroscience, Department of Neuropathology, Director, 神経病理研究部門, 副参事研究員 (90173921)

Co-Investigator(Kenkyū-buntansha) ISHIHARA Yoshihiro  Tokyo Metropolitan Institute for Neuroscience, Department of Neuropathology, Dir, 神経病理研究部門, 副参事研究員 (50079711)
ABE Satoshi  Niigata University, Brain Research Institute, Department of Neuropathology, Rese, 脳研究所神経病理, 助手 (90202663)
Project Period (FY) 1994 – 1995
KeywordsAutoimmune encephalomyelitis / Neural graft / Cytokines / Quantitative PCR / in situ RT-PCR
Research Abstract

In the present study, we investigated the role of cytokines secreted in the lesion in the central nervous system (CNS) and cytokine network.Since the amount of cytokine protein and mRNA expressed in the CNS is very little, it was very difficult to examine them by ordinary methods such as Western and Northern blot analysis.Instead, using the experimental autoimmune encephalomyelitis (EAE) and neural tissue grafting model, we tried to establish the quantitative analysis of cytokine mRNA after amplification by the PCR techniques, i.e., competitive PCR.
In acute and monophasic rat EAE,both pro-inflammatory )IFN-gamma and TNF-alpha) and anti-inflammatory (IL-10) cytokines reached a maximal at the early stage of EAE suggesting the nature of its clinical course.On the other hand, in the graft rejection processes, mRNA for cytolytic protein such as perforin and granzyme B was expressed markedly.These disease-specific cytokine secretion profile strongly suggest that the nature of the lesion formed in the CNS regulates the mode of the lesion repair by modulating the cytokine network formed in the brain.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Hirahara, H. et al.: "Long-term survival of cardiac allografts in rats treated before and after surgery with monoclonal antibody to CD2" Transplantation. 59. 85-90 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikarashi, Y. et al.: "Recipient-derived T cells participate in autoimmune-like hepatic lesions induced by graft-versus-host reaction." Autoimmunity. 20. 121-127 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田沼直之ら: "Competitive PCRによる自己免疫性脳脊髄炎におけるサイトカインmRNAの解析." 神経免疫学. 3. 48-49 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] Matsumoto, Y. et al.: "Characterizatoin of CD4-CD8-TCRαβ+T cells appearing in the subarachnoid space of rats with autoimmune encephalomyelitis." Eur. J. Immunol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shin, T. et al.: "The subarachnoid space as a site for precursor I cell proliferation and effector T cell selection in experimental autoimmune encephalomyelitis." J. Neuroimmunol.56. 171-177 (1995)

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      「研究成果報告書概要(和文)」より
  • [Publications] Tamura, N. et al.: "Pretreatment with T cell receptor peptide using conventional immunization protocol does not induce effective protection against autoimmune encephalomyelitis" Cell. Immunol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inomata, T., Izumi, T., Shibata, A., Tsuchida, M., Matsumoto, Y.& Abo, T.: "Anti-alphabeta T cell receptor antibody prevents the progression of experimental autoimmune myocarditis." Clin.Exp.Immunol.96. 470-475 (1994)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Ikarashi, Y., Abo, T., Kawai, K., Iiai, I., Watanabe, H., Suzuki, K., Matsumoto, Y., Omata, S.& Fujiwara, M.: "Expansion of imtermediate T-cell receptor cells in mice with autoimmune-like graft-versus-host disease." Immunology. 83. 205-212 (1994)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto, Y., Tsuchida, M., Hanawa, H.& Abo, T.: "T cell receptor peptide therapy for antoimmune encephalomyelitis.Stronger immunization is necessary for effective vaccination." Cell.Immunol.153. 468-478 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto, Y., Tsuchida, M., Hanawa, H.& Abo, T.: "Successful prevention and treatment of autoimmune encephalomyelitis with short-term administration of anti-T cell receptor alphabeta antibody" Immunology. 41. 1-7 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto, Y.& Abo, T.: "Lack of "determinant spread" to the minor encephalitogenic epitope in myelin basic protein-induced acute experimental autoimmune encephalomyelitis in the rat." Cell.Immunol.155. 517-523 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto, Y.: "Role of microglia in autoimmune encephalomyelitis." Neuropath.Appl.Neurobiol.20. 196-198 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuchida, M., Hanawa, H., Hirahara, H., Watanabe, H., Matsumoto, Y., Sekikawa, H & Abo.T.: "Identification of CD4-CD8-alphabeta T cells in the subarachnoid space of rats with experimental autoimmune encephalomyelitis.A possible route by which effector cells in vade the lesions." Immunology. 81. 420-427 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuchida, M., Matsumoto, Y.& Abo, T.: "Increased expression of LFA-1/ICAM-1 in TCR-positive lymphocytes infiltrating cardiac allograft by flow cytometric analysis in rats." Transplant.Proc.26. 3727-3730 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirahara, H., Tsuchida, M., Watanabe, T.Haga, M., Matsumoto, Y., Abo, T.& Eguchi, S.: "Long-term survival of cardiac allografts in rats treated before and after surgery with monoclonal antibody to CD2." Transplantation. 59. 85-90 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikarashi, Y., Matsumoto, Y., Omata, S.& Fujiwara, M.: "Recipient-derived T cells participate in autoimmune-like hepatic lesions induced by graft-versus-host reaction." Autoimmunity. 20. 121-127 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shin, T., Kojima, T., Tanuma, N., Ishihara, Y.& Matsunoto, Y.: "The subarachnoid space as a site for precursor T cell proiferation and effector T cell selection in experimental autoimmune encephalomyelitis." J.Neuroimmunol.56. 171-177 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gehrmann, J., Matsumoto, Y.& Kreutzberg, G.W.: "Microglia : Intrinsic immunoeffector cells in the brain." Brain Res.Rev.20. 269-287 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanuma, N., Abe, S., Shin, T., Kojima, T., Ishihara, Y.& Matsumoto, Y.: "Pretreatment with T cell receptor peptide using conventional immunization protocol does not induce effective protection against autoimmune encephalomyelitis." Cell.Immunol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto, Y., Abe, S., Tsuchida, M., Hirahara, H., Abo, T., Shin, T., Tanuma, N., Kojima, T.& Ishihara, Y.: "Characterization of CD4-CD8-TCRalphabeta+ T cells appearing in the subarachnoid space of rats with autoimmune encephalomyelitis." Eur.J.Immunol.(in press.). (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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