1994 Fiscal Year Annual Research Report

抗糖鎖抗体による運動神経疾患の発症機構の解明

Research Project

Project/Area Number	06680753
Research Institution	Tokyo Medical and Dental University
Principal Investigator	瀧孝雄東京医科歯科大学, 医学部, 助教授 (10046295)
Research Abstract	本研究では自己免疫疾患の1つであるGuillain Barre Syndrome(GBS)の発症機序を解明する目的で以下の実験を行った。GBS患者から分離されたC.jejuni(Penner type 19)のリポ多糖を分離精製し、神経成分とのリポ多糖との分子相同性を証明する。またGBSの亜型であり抗GQ1bガングリオシド抗体が検出されるFisher's syndrome患者から分離したC.jejuni(Penner type 2)のリポ多糖を分析しGQ1b構造が存在することを調べ、病原体と神経の分子相同性による末梢神経疾患の発症機構を分子レベルで解明する。 GBS患者には神経成分であるGM1ガングリオシドに対する抗体が上昇している。そのGBS患者から分離された先行感染菌C.jejuni(Penner type 19)のリポ多糖中にGM1ガングリオシドと同じ糖鎖が存在することをガスクロマト/質量分析、プロトンNMR,によって分析し,その構造(Galβ1-3GalNAcβ1-4(NeuAcα2-3)Galβ-)を化学的に明らかにした。またFisher's Syndrome患者の血清中に上昇している抗GQ1bガングリオシド抗体の抗原となる糖鎖構造が患者から検出されたC.jejuni(Penner type 2)のリポ多糖に存在すること免疫学的手法によって確認した。これらの成果から、病原体の感染によって産生された抗ガングリオシド抗体が神経筋接合部に作用して障害考えられる。その発症機序は、病原体成分(リポ多糖)と神経成分(ガングリオシド)との分子相同性に基づくことを分子レベルで明らかにした。

Research Products

(11 results)

All Other

All Publications (11 results)

[Publications] Taki,T.: "A new method for the detection of β1,4-galactosyltransferase activity in sera of cancer patiemts." Anal.Biochem.219. 104-108 (1994)
[Publications] Simbulan,C.M.G.: "Sulfate-and sialic acid-contaning glycolipids inhibit DNA polymerase α" Biochim.Biophys.Acta. 1205. 68-74 (1994)
[Publications] Simbulan,C.M.G.: "Sphingosine inhibits the synthesis of RNA primers by primerse in vitro." Biochemistry. 33. 9007-9012 (1994)
[Publications] Taki,T.: "Blotting of glycosphingolipids and phospholipids from a high performance thin-layer chromatography to a polyvinylidene difuoride membrane." Anal.Biochem.221. 312-316 (1994)
[Publications] Yuki,N.: "Penner'sserotype 4 of Campyrobacter jejuni has a lipopolysaccharede that bears a GM1 ganglioside epitope as well as one that bears a GD1a epitope." Infect.Immun.62. 2101-2103 (1994)
[Publications] Taki,T.: "A simple and quantitative purification of glycosphingolipids and phospholipids by TLC-blotting." Anal.Biochem.223. 232-238 (1994)
[Publications] Mstsuda,K.: "Structure of a novel phosphocholine containing glycoglycerolipid from Mycoplasma fermentans." J.Biol.Chem.269. 33123-33128 (1994)
[Publications] Yuki,N.: "Molecular mimicry between GQ1b ganglioside and lipopolysaccharide of group Campyrobacter jejuni isolated from patints with Fisher syndrome." Annal.Neurol.36. 791-793 (1994)
[Publications] Yamawaki,M.: "Accumulation of isobloboside and gnglio-N-tetraosyl ceramide blood B determinant in the hepatomas of female LEC rats" Biochim.Biophys Acta. (in press).
[Publications] Taki,T.: "Direct mass spectrometric analysis of glycospingdipid transferred to a PVDF membrane by TLD blotting" Anal.Biochem.(in press).
[Publications] Taki,T.: "An Improved method for the measurement and detection of sphingomyelinase" Anal.Biochem.(in press).

1994 Fiscal Year Annual Research Report

抗糖鎖抗体による運動神経疾患の発症機構の解明

Principal Investigator

瀧 孝雄 東京医科歯科大学, 医学部, 助教授 (10046295)

Research Products

[Publications] Taki,T.: "A new method for the detection of β1,4-galactosyltransferase activity in sera of cancer patiemts." Anal.Biochem.219. 104-108 (1994)

[Publications] Simbulan,C.M.G.: "Sulfate-and sialic acid-contaning glycolipids inhibit DNA polymerase α" Biochim.Biophys.Acta. 1205. 68-74 (1994)

[Publications] Simbulan,C.M.G.: "Sphingosine inhibits the synthesis of RNA primers by primerse in vitro." Biochemistry. 33. 9007-9012 (1994)

[Publications] Taki,T.: "Blotting of glycosphingolipids and phospholipids from a high performance thin-layer chromatography to a polyvinylidene difuoride membrane." Anal.Biochem.221. 312-316 (1994)

[Publications] Yuki,N.: "Penner'sserotype 4 of Campyrobacter jejuni has a lipopolysaccharede that bears a GM1 ganglioside epitope as well as one that bears a GD1a epitope." Infect.Immun.62. 2101-2103 (1994)

[Publications] Taki,T.: "A simple and quantitative purification of glycosphingolipids and phospholipids by TLC-blotting." Anal.Biochem.223. 232-238 (1994)

[Publications] Mstsuda,K.: "Structure of a novel phosphocholine containing glycoglycerolipid from Mycoplasma fermentans." J.Biol.Chem.269. 33123-33128 (1994)

[Publications] Yuki,N.: "Molecular mimicry between GQ1b ganglioside and lipopolysaccharide of group Campyrobacter jejuni isolated from patints with Fisher syndrome." Annal.Neurol.36. 791-793 (1994)

[Publications] Yamawaki,M.: "Accumulation of isobloboside and gnglio-N-tetraosyl ceramide blood B determinant in the hepatomas of female LEC rats" Biochim.Biophys Acta. (in press).

[Publications] Taki,T.: "Direct mass spectrometric analysis of glycospingdipid transferred to a PVDF membrane by TLD blotting" Anal.Biochem.(in press).

[Publications] Taki,T.: "An Improved method for the measurement and detection of sphingomyelinase" Anal.Biochem.(in press).

瀧孝雄東京医科歯科大学, 医学部, 助教授 (10046295)