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1996 Fiscal Year Final Research Report Summary

CONFIGURATION PATTERN OF CT-NUCLEOIDS IN DIATOMS

Research Project

Project/Area Number 06804056
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 系統・分類
Research InstitutionTOKYO GAKUGEI UNIVERSITY

Principal Investigator

MAYAMA Shigeki  TOKYO GAKUGEI UNIVERSITY,DEPARTMENT OF BIOLOGY,ASSISTANT, 教育学部, 助手 (40199914)

Co-Investigator(Kenkyū-buntansha) SHIHIRA Ikuko (ISHIKAWA I)  TOKYO GAKUGEI UNIVERSITY,DEP ARTMENT OF BIOLOGY,PROFESSOR, 教育学部, 教授 (50074638)
Project Period (FY) 1994 – 1996
Keywordstaxonomy / systematics / taxonomic characteristics / chloroplast / ct-nucleoid / nucleoid / diatom / pyrenoid
Research Abstract

Configurations of chloroplast-nucleoids (ct-nucleoid) were examined with DAPI-stained diatoms (16 genera, 50 species). The configuration was ring-type in all species except for several Pinnularia and Nitzschia species, in which scattered DAPI-fluorescent globules and short strings of DAPI-fluorescence were observed respectively. As DAPI-fluorescent globules in Pinnularia disappeared by DNase treatment, they were confirmed to contain DNA.These globules were confirmed to be located in the chloroplast by confocal laser microscopy and observations of the sections of the cells embedded in Technovit 7100. These globules corresponded to the discrete areas of thylakoids stack in TEM observation. No membranous structure was detected in these areas. The chloroplasts were isolated and then these globules also isolated. The size of globules were similar to that of each beads in ring-type nucleoid. Employing proteinase K treatment, these globules were lysed and then DNA was diffused. These observat … More ions and experiments prove that the globules scattered throughout the chloroplast are the nucleoids to be a complex of DNA and protein. In Pinnularia, 11 species have the ring-type ct-nucleoid and 9 species have the scattered-type nucleoid as well as the ring-type and 1 species has only the scattered-type. Species with the scattered-type are limited in diatoms of P.sect. Pinnularia and P.sect. Divergentissima. In Nitzschia sigmoidea, we observed that small rectangular pyrenoids were scattered throughout the chloroplasts by propion-carmine staining method. In TEM section, they were lens shape, which was typical in diatoms. Specific localization of Rubisco was detected in this structure using indirect immuno-fluorescence microscopy and electron microscopy with immuno-gold. Although the ring-type nucleoid was not observed in this species, linear DAPI-fluorescence was observed along both sides of the pyrenoids and they seem to be the ct-nucleoids. The configurations of ct-nucleoids in diatoms are diversified as in diatom frustules, and seem to reflect their systematics. Less

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Mayama,S.and Shihira-Ishikawa,I.: "Putative nuclecids scattered in chloroplast of Pinnularia nobilis(Bacillariophyceae)" Jpn.J.Phycol.42. 437-441 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shihira-Ishikawa,I.and Ohsu,T.: "Distribution and migrartion of mitochondria in centric diatom Pleurosira laevis(Ehenberg)Compere." Diatom. 11. 1-7 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 真山なぎさ・真山茂樹: "顕微鏡下の操作におけるまつ毛の代用品" Diatom. 11. 88-88 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mayama,S.: "Enaotia valida and E.psendovelida spec.rov.(Bacillariophyceae) : Ultrastrhcture of the valve and girdle." Nova Hedwigia. 65.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mayama, S.and Shihira-Ishikawa, I.: "Putative nucleoids scattered in chloroplast of Pinnularia nobilis (Bacillariophyceae)." Jpn.J.Phycol.42. 437-441 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shihira-Ishikawa, I.and Ohsu, T.: "Distribution and migration of mitochondria in centric diatom Pleurosira laevis (Ehrenberg) Compere." Diatom. 11. 1-7 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mayama, N.and Myayama, S.: "The substitusion of a "Denter-Systema" toothbrush fiber for an eyelash in micromanipulation." Diatom. 11. 88 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mayama, S.: "Eunotia valida and E.pseudovalida spec. nov. (Bacillariophyceae) : Ultrastructure of the valve and girdle." Nova Hedwigia.65(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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