1995 Fiscal Year Final Research Report Summary
Investigation for factors modulating differentiation of rat Ito cells
| Project/Area Number |
06807013
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY |
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Principal Investigator |
KOYATA Hirohisa Toyama Medical & Pharmaceutical Univ. Biochemistry, Assistant professor, 医学部, 助教授 (60211249)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Ito cell / Extracellular matrix / Retinoids / Type I collagen alpha1 chain |
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| Research Abstract |
Hepatic sinusoidal Ito cells are the major site for retinoid storage. In another aspect, the cells that lost intracellular retinoid have been referred to myofibroblast-like cells growing rapidly and producing the components of extracellular matrix during or following alcoholic hepatitis. Clonal cells were isolated from rat liver cells dispersed by portal perfusion with collagenase and pronase E followed by Metrizamide density gradient centrifugation. Since the clonal cells with a doubling time of 40 hours on plastic substratum showed immunohistochemically detectable desmin in their cytoplasm and a high level of type I collagen alpha1 chain (alpha1(I)) mRNA,they seemed to originate a myofibroblast-like Ito cells. Effects of cell adhesion matrix on alpha1(I) mRNA levels of Ito cells were investigated by Northern blotting using freshly isolated Ito cells, primary culture Ito cells and the clonal cells. Levels of alpha1(I) mRNA of both primary culture Ito cells and the clonal cells on plastic substratum were 40 and 800 times higher than that of freshly isolated Ito cells as a control, respectively. The clonal cells adherent to a gel matrix, Matrigel, derived from the Engelbreth-Holm-Swarm murine tumor decreased their alpha1(I) mRNA level (40% of that of cells on plastic) and proliferated about 4 times slowly. These results suggest that adhesion matrices of Ito cell may play important roles for modulation of Ito cell differentiation.
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