1995 Fiscal Year Final Research Report Summary
Study on intogration of EBV genome into host DNAs of EBV positive lymphoma cells derived from SCID mouse transplantation
| Project/Area Number |
06807018
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Kitasato University |
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Principal Investigator |
TAKANO Yasuo Kitasato Univ., Sch.of Medicine, Assoc.Professo, 医学部・病理学教室, 助教授 (60142022)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Epstein-Barr Virus / SCID mouse / Molignant lympbhma |
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| Research Abstract |
The investigation for integration of Epstein-Barr virus (EBV) genome into host DNAs of 30 cases of EBV positive lymphoma cells derived from SCID mouse transplantation system has been done since the beginning of 1994. DNAs extracted from these cases were cut by 3 kinds of restriction enzymesand then they were electrophoresed in 3% Nusieve GM soft agar gel. After reextraction of DNAs less than 1 kilo-base in lenght, PCR amplification was performed using the DNAs as template. Primers employed were 6 kinds for Alu-tandem repeats as sense and 3 kinds for terminal repeat of EBV as anti-sense. Single bands between 400 and 700 in length appeared in 3 cases. Direct sequencing of these DNAs revealed that DNAs apart from host DNA or EBV DNA were obtained. It was speculated to be DNAs derived from mouse DNA.Repeated examination brought same results. It passed to the middle or '96 in these condition.
Another technique published by Gulley et al. (Cancer 70 : 191-195) was considered to investigate the integration of EBV DNA to host DNA.However, the study has not benn going well because specific probes for the study were not available.
On the basis of accumulated knowledge and technique for EBV,we investigated the role of EBV on carcinogenesis in EBV positive gastric cancer cases and published the results at the Journal of Pathology. Moreover, a study on EBV genotype in these cases was performed and its results was submitted to the Journal of Pathology.
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