Development of the rapid detection method of methicillin resistant Staphylococcus aureus (MRSA)

1995 Fiscal Year Final Research Report Summary

• Project/Area Number: 06807040
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Public health/Health science
• Research Institution: Osaka Prefectural Institute of Public Health
• Principal Investigator: SAKAGAMI Yoshikazu Osaka Prefectural Institute of Public Health, Pharmaceutical Affairs, Senior Researcher, 薬事指導部, 主任研究員 (50192084)
• Co-Investigator(Kenkyū-buntansha): KAJIMURA Keiji 大阪府立公衆衛生研究所, 薬事指導部, 主任研究員 (40250336)
• Project Period (FY): 1994 – 1995
• Keywords: MRSA / NEW DNA CHEMIPROBE / mec A / PCR

Research Abstract

[Object] The development of a rapid detection method of MRSA was performed for the regulation of MRSA infection, one of the severe problem in hospital of Japan.
[Methods] MIC values against methicillin were measured with 130 strains of MRSA isolated from 7 hospitals in Japan. Repeated cultivation was performed for 10 times (for 10 days) for obsevation of MRSA-resistant pattern. A rapid detection method of MRSA was developed by using NEW DNA CHEMIPROBE method with using MRSA and methicillin-sensitive(resistant-falling strains ; MIC value of methicillin was below 12.5 mu g/ml) strains, and compared with the detection method of mec A gene by PCR.
[Results] The detction method by NEW DNA CHEMIPROBE on using MRSAs and methicillin-sensitive(resistant-falling starins) strains was almost similar to the detection method of of mec A gene by PCR.
[Conclusion] From the test results, it would suggest that the detection method by NEW DNA CHEMIPROBE had good detection sensitivity and almost equal to the detection of mec A gene by PCR.Furthermore, it would contribute to MRSA detection field.