1995 Fiscal Year Final Research Report Summary
Immuno-biochemical studies on a novel DNA tyge of bacteria from a kawasaki ob's ease patient and the role of a novel protease
| Project/Area Number |
06807065
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | KITASATO UNIVERSITY |
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Principal Investigator |
OHTSU Eiji Kitasato Univ., Medicine, Associate Professor, 医学部・小児科, 助教授 (80137934)
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| Co-Investigator(Kenkyū-buntansha) |
HORI Shin-ichiro Tokyo Metropolitan Institute For Neuroscience, Molecular Neurobiology Dept., Sta, 生化学, 副参事 (50073086)
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| Project Period (FY) |
1994 – 1995
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| Keywords | anariant streptococcus / Kawasaki disease / specific surface antigen / PNA-DNA hybridization / 16s RNA gene / RFLP / antigens / モノクローナル抗体 |
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| Research Abstract |
1. The genetic indentification of a variant streptococcal strain irolated from a Kawasaki disease child has benn successfully performed and the possible zixrulent antigens including a novel type of protease have been-clarified.
2. The PNA-DNA hy brkidization test in a strimgent condition confirmed that the possibly causatine organism certainly differed from a serils of oral streptococci.
3. The 16s RNA gene was sequenced and 5 mutation aites were elucidated to be useful for RFLP detectine diagnosis of a series of reproducibly virnlent strains.
4. The virulent antigens were regroduibly detected in the sera of Kawasaki poctients also using the specific monoclonal antibodies.
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