PHYSIOLOGICAL FUNCTION OF Rh-BLOOD GROUP SYSTEM.CONTRIBUTION OF D-ANTIGEN FOR TRANSPORT SYSTEMS.

1995 Fiscal Year Final Research Report Summary

• Project/Area Number: 06807089
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: YAMAGUCHI UNIVERSITY
• Principal Investigator: YOSHIDA Hisahiro YAMAGUCHI UNIVERSITY SCHOOL OF MEDICINE DEPARTMENT OF PHARMACY ASSOCIATE PROFESSOR, 医学部・附属病院, 助教授 (30135587)
• Co-Investigator(Kenkyū-buntansha): KAMIYA Akira YAMAGUCHI UNIVERSITY SCHOOL OF MEDICINE DEPARTMENT OF PHARMACY PROFESSOR, 医学部・附属病院, 教授 (90124792)
• Project Period (FY): 1994 – 1995
• Keywords: RED-BLOOD-CELL-GHOST / Rh-BLOOD GROUP SYSTEM / TRANSPORT SYSTEMS / D-ANTIGEN

Research Abstract

To clarify the physiological functions of the Rh-blood group system, we investigated the contribution of D-antigen to various transport systems using red-blood-cell-ghosts (RBC-ghosts) of various Rh-phenotypes. Namely, glucose, p-aminohippuric acid, tetraethyl-ammmonium, and glycylsarcosine were used as the model drugs for the transport system of glucose, organic anion, organic cation, and dipeptide, respectively. Drug uptake experiments were done by the rapid membrane filtration method using 3H- or 14C- labeled drugs.
The uptake of p-aminohippuric acid was increased with an outside-to-inside proton gradient, and that of the glycylsarcosine was increased with an inside-to-outside proton gradient. However, the uptake of these drugs was not affected by the Na+-K+gradient, drug concentrations, or inhibitors. Anti-D antibodies did not affect the uptake of these drugs with RBC-ghosts. No differences in the uptake properties were observed with the Rh-phenotype. These findings showed that the active transport systems and D-antigen did not contribute to these processes. Thus, the effect of the proton gradient observed for p-aminohippuric acid and glycylsarcosine may be due to the pH-partition theory.