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1995 Fiscal Year Final Research Report Summary

ISOLATION AND CHARACTERIZATION OF AN LIPOPOLYSACCHARIDE (LPS)-RESISTANT GENE FROM AN LPS-REISITANT MACROPHAGE-LIKE CELL LINE

Research Project

Project/Area Number 06807171
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionNATIONAL INSTITUTE OF HEALTH

Principal Investigator

AMANO Fumio  NATIONAL INSTITUTE OF HEALTH DEPARTMENT : DEPARTMENT OF BIOCHEMISTRY AND CELL BIOLOGY TITLE : SENIOR RESEARCHER, 細胞化学部, 主任研究官 (90142132)

Project Period (FY) 1994 – 1995
Keywordslipopolysaccharide (LPS) / LPS-resistance / resistant gene / J774.1 macrophage cell line / RAW 264.7 macrophage cell line / 変異株 / リポ多糖(LPS)
Research Abstract

An assay method was newly developped for evaluation of LPS-resistance : Both 10 mug/ml cycloheximide and 10 ng/ml LPS induced the severe cell damages in an LPS-sensitive macrophage-like cell, JA-4, a subline of J774.1, andafter incubation at 37゚C for 4th.However, LPS1916 cell line, a highly resistant cell line to LPS,was not damaged at all, even after incubation with cycloheximide and 1 mug/ml LPS.This method was sensitive and rapid for estimation of LPS-resistance, as compared with a cell growth assay or a colony-formation assay.
Trials to transfect DNA to JA-4 cells were not successful with calcium phosphate method, electro-poration method or lipofection method.A preliminary experiment is being performed by using another macrophage-like cell line, RAW 264.7, which is sensitive to LPS,as a receipient of the cDNAa from LPS1916 as well as JA-4 cells, because a recent report from other laboratory suggested that RAW 264.7 cells expressed luciferase gene after transfection of the gene.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Kura, F.: "Difference in Legionella pneumophila growth permissiveness between J774.1 murine macrophage-like JA-4 cell and lipopolysaccharide (LPS)-resistant mutant cells, LPS1916, after stimulatioh with LPS." Infection and Immunity. 62. 5419-5423 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Amano,F.: "Heterogeneity in lysosomal fusion with phagocytic vesicles and cell membrane in non-phagocytosing Guinea pig polymorphonuclear leukocyte." Japanese Journal of Medical Science and Biology. 47. 179-193 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura, M. R.: "Resistance to Mycobacterial in fection and cytokine production in mouse macrophages." Ann. New York Academy of Science. 730. 313-314 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inoue, S.: "Intracellular killing of Listeria monocytogenes in the J774.1 macrophage-like cell line and the lipopolysaccharide (LPS)-resistant mutant LPS1916 cell line defective in the generation of reactive oxygen intermediates after LPS treatment." Infection and Immunity. 63. 1876-1886 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Amano, F.: "Inhibition of zymosan-induced macrophage functions by diisopropyl-fluorophosphate (DFP) in a macrophage-like cell line, RAW264.7 cells." Proteases involved in cancer. 129-134 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Amano, F: "Improved detection of nitric oxide radical (NO・) production in an activated macrophage culture with a radical scavenger, carboxy PTIO, and Griess reagent." FEBS Letters. 368. 425-428 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 天野富美夫: "ライソゾームの細胞内輸送、「ライソゾームの基礎とライソゾーム病」(小川和朗編)" 学際企画, 202 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 天野富美夫: "マクロファージによる潜伏感染細菌排除機構、「LIP」(倉田毅,天野富美夫編)" 菜根出版, 213 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kura, F., Suzuki, K., Watanabe, H., Akamatsu, Y., and Amano, F.: "Difference in Legionella pneumophila growth permissiveness between J774.1 murine macrophage-like JA-4 cells and lipopolysaccharide (LPS)-resistant mutant cells, LPS1916, after stimulation with LPS." Infection and Immunity.62. 5419-5423 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Amano, F., Hashida, R.and Mizuno, D.: "Heterogeneity in lysosomal fusion with phagocytic vesicles and cell membrane in non-phagocytosing Guinea pig polymorphonuclear leukocyte." Japanese Jornal of Medical Science and Biology. 47. 179-193 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, M.R.and Amano, F.: "Resistance to Mycobacterial infection and cytokine production in mouse macrophages.in "Microbial Pathogenesis and Immune Response" .(Ades, W., Rest, R.F.and Morse, S.A., eds.)" New York Academy of Science, New York. vol 730. 313-314 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue, S., Itagaki, S-I.and Amano, F.: "Intracellular killing of Listeria monocytogenes in the J774.1 macrophage-like cell line and the lipopolysaccharide (LPS)-resistant mutant LPS1916 cell line defective in the generation of reactive oxygen intermediates after LPS treatment." Infection and Immunity. 63. 1876-1886 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Amano, F.: "Inhibition of zymosan-induced macrophage functions by diisopropyl-fluorophosphate (DFP) in a macrophage-like cell line, RAW264.7 cells in "Proteases involved in cancer" , Suzuki, M.and Hiwasa, T.eds." Monduzzi Editore, Bologna.129-134 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Amano, F.and Noda, T.: "Improved detection of nitric oxide radical (NO・) production in an activated macrophage culture with a radical scavenger, carboxy PTIO,and Griess reagent." FEBS Letters.368. 425-428 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Amano, F.: Intracellular traffic of lysosomes.In "Lysosomes in Fundamental Biology and Lysosomal Disease" Ogawa, K.ed.Gakusai Kikaku, Tokyo (Japanese), 93-105

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Amano, F.: Mechanisms of anti-microbial infections of latent and persistent bacteria in macrophages.In "LIP : Latent and Persistent Infection of Microbes-Host Responses- "., Kurata, T.and Amano, F., eds.Saikon Shuppann, Tokyo (Japanese), 57-69 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Amano, F.: Methods in receptor analysis."Protocols for Cell Biology" , Hotta, Y.and Ohkuma, S., eds.Hirokawa Shotenn, Tokyo, (in press) (Japanese),

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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