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1995 Fiscal Year Final Research Report Summary

Study on the Vibro flagellar motor as the mechanosensor

Research Project

Project/Area Number 06808064
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionNagoya University

Principal Investigator

HOMMA Michio  Nagoya Univ, Dep.of Mol.Biol., associate professor, 理学部, 助教授 (50209342)

Project Period (FY) 1994 – 1995
Keywordsion channel / phenamil / Vibrio / flagella / motor
Research Abstract

Certain marine Vibrio species swim in seawater propelled by a polar flagellum and swarm over surfaces using numerous lateral flagella. The polar and the lateral flagellar motors are powered by sodium-and proton-motive forces, respectively. The lateral flagella are produced in media of high viscosity, and the relevant viscosity sensor is the polar flagellum. The cell might monitor either the rotation rate of the flagellar motor or mechanical force applied against the flagellum. To test these possibilities, we examined the effects of amiloride and its derivatives, which inhibit the rotation of the dodium-driven motor, on lateral flagella gene (laf) expression in Vibrio parahaemolyticus. Phenamil, an amiloride analogue that inhibits swimming at micromolar concentrations, induced laf transcription in media devoid of viscous agents in a dose-dependent manner. The relationship between the average swimming speed and laf induction in the presence of various concentrations of phenamil was very similar to that observed when viscosity was changed. These results indicate that marine Vibrio senses decrease in the rotation rate of (or the sodium influx through) the polar flagellar motor as a trigger for laf induction.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] I,Kawagishi: "The sodium-driven polar flugellar motor of marine Vibrio as the mechanos'ensor that regalates laterd flegellar expression" Molecular Microbiology. (発表予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] I,Kawagishi: "Isolation of the polar and lateral flagellum-defective mutants in vibrioalginol yticus and identification of their flagellar driving energy sources" Journal of Bacteriology. 177. 5158-5160 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] I,Kawagishi: "Removal of the periplasmic DNA ae before electroporation enhances efficiency of fvous formaation in the marine bacferium Vibrio alginolyticus." Microbiology. 140. 2355-2361 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawagishi, I., Imagawa, M., Imae, Y., McCarter, L., and Homma, M.: "1995. The sodium-driven polar flagellar motor of marine Vibrio as the mechanosensor that regulates lateral flagellar expression." Mol.Microbiol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawagishi, I., Maekawa, Y., Asumi, T., Homma, M., and Imae, Y: "Isolation of polar and lateral flagellar defective mutants in Vibrio alginolyticus and characterization of the two flagellar systems." J.Bacteriol.177. 5158-5160 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawagishi, I., Okunishi, I., Homma, H., and Imae, Y.: "Removal of the periplasmic DNase before electroporation enhances efficiency of transformation in the marine bacterium Vibrio alginolyticus." Microbiology. 140. 2355-2361 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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