1995 Fiscal Year Final Research Report Summary
Transplantation of Endothelial Cells Sheets by Utilizing Thermoresponsive Intelligent Material
| Project/Area Number |
06808093
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
NEGISHI Naoki Tokyo Women's Medical College, Dept.of Plastic & Reconstructive Surgery, Assist.Professor, 医学部, 講師 (30218268)
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| Co-Investigator(Kenkyū-buntansha) |
SOEJIMA Kazutaka Tokyo Women's Medical College, Dept.of Plastic & Reconstructive Surgery, Clinica, 医学部, 助手 (00246589)
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| Project Period (FY) |
1994 – 1995
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| Keywords | endothelial cells / cultured cells transplantation / intelligent material / thermoresponsive polymer / poly (N-isopropylacrylamide) / collagen-based artificial dermis |
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| Research Abstract |
The purpose of this study is to evaluate the effect of cultured endothelial cells on angiogenesis in vivo.
The endothelial cells obtained from thoracic aorta in Wistar male rats (weighting 100 to 200g) were isolated and cultured by explant technique on the thermoresponsive dishes, which are tissue culture polystirene dishes binded with thermoresponsive poly (N-isopropylacrylamide). Using the thermoresponsive dishes, a confluent layr of endothelial cells can be detached as an intact sheet by low temparature treatment lowered to 5ﾟC) without enzymatic treatment. The cultures were characterized by the the presence of Weibel Palade body of endothelial cells with electron microscopy.
Twenty Wister male rats were used for this experiment. Full thickness skin defects (3X3cm) were made on the back of rats, and artificial dermis were grafted. The rats were divided into two groups. Control group was artificial dermis alone. The artificial dermis with the sheet of cultured endothelial cells laid on the graft beds (anather group). Histological examinations were performed at 5,10,15, and 20 days after surgery. It was found that the vascularization of the artificial dermis was significantly enhanced in the group of transplantation of endothelial cells.
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