1994 Fiscal Year Final Research Report Summary

ヒト・ゲノム解析研究

Research Project

Project/Area Number	06NP0401
Research Category	Grant-in-Aid for Creative Basic Research
Allocation Type	Single-year Grants
Research Institution	Osaka University
Principal Investigator	松原謙一大阪大学, 細胞生体工学センター, 教授 (20037394)
Co-Investigator(Kenkyū-buntansha)	大木操国立がんセンター研究所, 部長 (00158792) 中村祐輔東京大学, 医科学研究所, 教授 (70217909) 吉田光昭東京大学, 医科学研究所, 教授 (80012607) 吉川寛奈良先端科学技術大学院大学, 教授 (70019876) 榊佳之東京大学, 医科学研究所, 教授 (10112327)
Project Period (FY)	1991 – 1995
Keywords	ヒト・ゲノム / ゲノム構造解析 / ゲノム機能解析 / cDNA / DNA解析技術 / モデル生物 / 生物情報科学 / 塩基配列決定
Research Abstract	我々自身である人間の理解を深め、人類の健康と幸福に役立つ知識と技術の生産に貢献する。それと共にDNA研究を挺子にした生命の研究と情報科学の融合した新しい研究分野の出現に早くから対処する。このような視点に立ってヒト・ゲノム解析研究は発足し、生命の設計図であるゲノムの構造と機能を解析研究し、そこに包含される遺伝情報の理解を目指して進められている。この線に沿って、総括班ならびに3つの研究班を組織して研究の推進を図り次の成果を得た。 1.総括班。全体としての活動を円滑に進めるため各種会合の企画立案調整、機器の計画的購入と配分、情報交流、調査などを積極的に進めた。また「ヒトゲノム解析と共役した遺伝病家系研究」、「日本における生物学的研究材料保存・供給体制のあり方について」「社会との接点の問題研究」について三つの推進小委員会を設けて調査研究、提言作成の作業を進めている。この他に公開シンポジウム、国際シンポジウム、講習会、ジャーナリストのためのチュートリアルとニュースレター発行等の広報活動を行った。 2.ヒト・ゲノム構造解析班。特定の染色体あるいは染色体の機能領域の構造解析を中心に研究し、必要な技術の開発研究を行うのを主目的とする。ヒト染色体21番11番につき物理地図を作製した。また、染色体3,6,8,11,X,Yにつき高密度のコスミドマップ作製を進めた。これらの成果を活用して、各種疾病遺伝子の発見入手に向けてエキソントラップ法などによる遺伝子存在部位の確認作業を進める一方、特定領域として免疫グロブリン遺伝子H鎖V領域とHLA遺伝子群を選び詳細なマップ作りと遺伝子の同定を実行した。これは、来るべきシーケンシング時代のモデルとなる先導的研究である。 3.cDNA解析班。ヒトゲノムの機能解析のため、遺伝子として発現する部分に研究対象を絞り、ヒトの各種組織におけるcDNAの存在頻度情報の収集と全シーケンス情報の収集を行う。ヒトの各種臓器や細胞の中で発現している全遺伝子をカタログ化し、その発現頻度を解析し情報化する「3'特異的cDNAライブラリー解析」を進めそのデータベースをさらに充実させて、ゲノム研究を構造解析と機能解析の両面から行う研究に貢献した。また100を越えるcDNAの全シーケンスの収集を達成しており、我国からのゲノム研究へのユニークな貢献となっている。良質なcDNAライブラリーの作成やその迅速な分類、カタログ化の手法、5'を含むクローンの選択的入手法などがほぼ完成した。これと共に、線虫をモデル系とするゲノムの構造解析と発生ステージで発現する遺伝子収集を共役させる研究が大いに進展をみせた。また、大容量のデータ整理などにおける新しい手法が種々開発されると共に、cDNAのゲノムへのマッピングを可能にするいろいろな技術開発が行われ、一部でcDNAマッピングのデータ生産活動が始まった。 4.DNA解析技術開発班。巨大なゲノムを解析するための技術開発とモデル生物(分裂酵母と枯草菌)を用いる大規模シーケンシングの効率化に関する手法の検討、解析の国際協力の組織化の研究。DNAの取り扱い技術とマーカーDNAの染色体上のマッピングに関わる技術開発を進め、特に、色素プローブを用いる染色体上への位置決め技術にさまざまな進歩を示すことができた。また、ゲノムスキャンニング法の応用、T3ファージによるDNA欠失セットの作成技術、YACの安定化、超高分子DNAの電場による分離技術の確立などに成果があがった。これと並んで、枯草菌、分裂酵母のゲノムを対象に国際協力によって大量シーケンシングを実行する研究を進めて、ゲノムデータベース作成への貢献が長足の進歩を遂げた。

Research Products
(107 results)

All Other

All Publications (107 results)

[Publications] Matsuo,A.(Isobe,M.): "Immunohistochemical localization in the rat brain of an epitope corresponding to the fibroblast growth factor receptor-1" Neuroscience. 60. 49-66 (1994)
[Publications] Isobe,M.: "Chromosomal localization of an SH2 containing tyrosine phosphatase (SH-PTP)gene to chromosome 12q24.1" Oncogene. 9. 1751-1753 (1994)
[Publications] Isobe,M.: "Assignment of the true and processed genes for human glycine decarboxylase to 9p23-24 and 4q12" Biochem.Biophys.Res.Comm.203. 1483-1487 (1994)
[Publications] Virgilio,L.(Isobe,M.,Imai,T.,Ikemura,T.): "Identification of the lymphocyte-specific tcl-1 gene involved in T cell malignancies" Proc.Natl.Acad.Sci.USA,. (in press).
[Publications] Ando,A.(Inoko,H.): "Cloning of a new kinesin-related gene located at the centromeric end of the human MHC region" Immunogenetics. 39. 194-200 (1994)
[Publications] Koshizaka,T.(Inoko,H.): "A new HLA-DPB1 allel,DPB1*SUT(DPB1*4701)" Tissue Antigens. 43. 50-53 (1994)
[Publications] Inoko,H.: "Role of human leukocyte antigens and T lymphocytes and in the immune response" Annals of Cancer Research and Therapy. 2. 173-191 (1994)
[Publications] Shindo,Y.(Inoko,H.): "HLA-DRB1 typing of Vogt-Koyanagi-Harada's disease by PCR-RFLP and the strong association with DRB1*045 and DRB1*0410" British Opthal. 78. 223-226 (1994)
[Publications] Shindo,Y.(Inoko,H.): "Complete association of the HLA DRB1*4 and DQB1*04 alleles with Vogt-Koyanagi-Harada's disease" Hum.Immunol.39. 169-176 (1994)
[Publications] Onuma,H.(Inoko,H.): "Association of HLA-DRB1*0501 with early-onset Graves'disease in Japanese" Hum.Immunol.39. 195-201 (1994)
[Publications] Ishihara,M.(Inoko,H.): "Molecular genetic studies of HLA class II alleles in sarcoidosios" Tissue Antigens. 43. 238-241 (1994)
[Publications] Ogawa,K.(Inoko,H.): "A novel HLA-DPB1 allele,DPB1*3601(DPB1*KT)" Tissue Antigens. 44. 134-136 (1994)
[Publications] 成瀬妙子(猪子英俊): "DNAによるHLAタイピングの基礎(1)HLA領域を構成する遺伝子その塩基配列" Organ Biology. 1. 95-102 (1994)
[Publications] Toda,T.(Imai,T.): "Isolation and characterization of a novel gene encoding nuclear protein at a locus(D11S636)tightly linked to multiple endocrine neoplasia type 1(MEN1)" Hum.Molec.Genet.3. 465-470 (1994)
[Publications] Imai,T.: "Assignment of the human ribosomal protein S25 gene(RPS25)to chromosome 11q23.3 by sequence analysis of the marker D11S456" Genomics. 20. 142-143 (1994)
[Publications] Michael R.James(Imai,T.,Nakamura,Y.): "A radiation hybrid map of 506 STS markers spanning human chromosome II" Nature genetics. 8. 70-76 (1994)
[Publications] Iida,A.(Imai,T.,Nakamura,Y.): "Allelic losses on chromosome 11q13 in aldosterone-producing adrenal tumors" Genes Chromo.Cancer. (in press).
[Publications] Mitani,K.(Ohki,M.): "Generation of the AMLI-EVII fusion gene in the t(3;21)(q26:q22)causes blastic crisis in chronic myelocytic leukemia" EMBO J.13. 504-510 (1994)
[Publications] Ichikawa,H.(Ohki,M.): "An RNA-binding protein gene,TLS/FUS,is fused to ERG in human myeloid leukemia with t(16:21)chromosomal translocation" Cancer Res.54. 2865-2868 (1994)
[Publications] Takeda,O.(Kaneko,Y.): "There may be two tumor suppressor genes on chromosome arm 1p closely as sociated with biologically distinctsubtypes of neuroblastoma" Genes Chromo.Cancer. 10. 30-39 (1994)
[Publications] Nishi,H.(Kimura,A.): "Possible gene dose effect of a mutant cardiac β-myosin heavy chain gene on the clinical expression of familial hypertrophic cardiomyopathy" Biochem.Biophys.Res.Commun.200. 549-556 (1994)
[Publications] Kuwano,A.(Kondo,I.): "Precise chromosomal locations of DRPLA,F8vWF and PTHLA in human chromosome 12p" Hum.Genet.,. (in press).
[Publications] Tanahashi,H.(Sakaki,Y.): "Sixty news STSs(Sequence-Tagged Sites)of human chromosome21" DNA Res.1. 85-89 (1994)
[Publications] Zhao,N.(Sakaki,Y.): "Cloning and sequence analysis of the human SNAP25cDNA" Gene. 145. 313-314 (1994)
[Publications] Suzuki,H.(Sakaki,Y.): "Genomic analysis of a NF1-related psendogene on human chromosome 21" Gene. 147. 277-280 (1994)
[Publications] Ito,T.(Sakaki,Y.): "Flourescent differential display:arbitrarily primed RT-PCR fingerprinting on an automnated DNA sequencer" FEBS Letter. 351. 231-236 (1994)
[Publications] Zhao,N.(Sakaki,Y.): "High-density cDNA filter analysis:a novel approach for large-scale,quantitative analysis of gene expression" Gene,. (in press).
[Publications] Shimizu,N.: "Detection of the EGF receptor gene amplification in the human squamous cell carcinomas using FISH technique" Jpn.J.Cancer Res.85(6). 567-571 (1994)
[Publications] Wang,Y.(Shimizu,N.): "The human genes for desmogleins(DSG1 and DSG3)are located in a small region on chromosome 18q12" Genomics. 20(3). 492-495 (1994)
[Publications] Fujita,N.(Shimizu,N.): "Genetic origin analysis of hydatidiform mole and non-molar abortion using the polymerase chain reaction method" Acta Obstet.Gynecol.Scand.73(4). 719-725 (1994)
[Publications] Minoshima,S.(Shimizu,N.): "Mapping of the gene for human xanthine dehydrogenase (oxidase)(XDH)to the band p23 of chromosome 2" Cytogenet.Cell Genet.68(1-2). 52-53 (1995)
[Publications] Amagai,M.(Shimizu,N.): "Assignment of the human genes for desmocollin 3 and desmocollin 4 to chromosome 18q12" Genomics. (in press).
[Publications] Sakai,K.(Shimizu,N.): "Human rebosomal RNA gene cluster:Identification of the proximal end containing a novel tandem repeat sequence" Genomics. (in press).
[Publications] Kubota,R.(Shimizu,N.): "Mapping of the human gene for Pmel 17(D12S53E)to chromosome 12q13-q14" Genomics. (in press).
[Publications] Kudoh,J.(Shimizu,N.): "Localization of the human desmoyokin/AHNAK gene to chromosome 11q12 by somatic cell hybrid analysis and flourescence in situ hybridization" Cytogenet.Cell Genet.,in press.
[Publications] Nakano,R.(Tsuji,S.): "A novel mutation in Cu/Zn superoxide dismutase gene in Japanese familial amyotrophic lateral sclerosis" Biochem.Biophys.Res.Commun.200(2). 695-703 (1994)
[Publications] Okuizumi,K.(Tsuji,S.): "ApoE-_ε4 and early-onset Alzheimer's" Nature Genet.7(1). 10-11 (1994)
[Publications] Takano,H.(Tsuji,S.): "Effect of intravenous immunoglobulin in Lambert-Eaton myasthenic syndrome with small-cell lung cancer:Correlation with the titer of anti-voltage-gated calcium channel antibody" Muscle Nerve. 17. 1073-1075 (1994)
[Publications] Koide,R.(Tsuji,S.): "Hereditary progressive dystonia with marked diurnal fluctuation caused by mutations in the GTP cyclohydrolase I gene" Nature Genet.8. 236-242 (1994)
[Publications] Sequeiros,J.(Tsuji,S.): "Genetic linkage studies of Machado-Joseph disease with chromosome 14q STRPs in 16 Portuguese-Azorean Kindreds" Genomics. 21. 645-648 (1994)
[Publications] Fujigasaki,H.(Tsuji,S.): "Mutational analysis of the amyloid precursor protein gene in Japanese familial Alzheimer's disease kindreds" Hum.Genet.93. 460-462 (1994)
[Publications] Naruse,S(Tsuji,S): "Structures of the human and mouse growth inhibitory factor-encoding genes" Gene. 144. 283-287 (1994)
[Publications] Ichinose,H.(Tsuji,S.): "Unstable expansion of CAG repeat in hereditary dentatorubral-pallidoluysian atrophy(DRPLA)" Nature Genet.6(1). 9-13 (1994)
[Publications] Kobayashi,K.(Nakagome,Y.): "PCR analysis of the Y chromosome long arm in azoospermic patients:evidence for a second locus required for spermatogenesis" Human Molec.Genet.3. 1965-1967 (1994)
[Publications] Nakahori,Y.(Nakagome,Y.): "A locus of the candidate gene family for azoospermia factor(YRRM2)is polymorphic with a null allele in Japanese males" Human Molec.Genet.3. 1710 (1994)
[Publications] Iida,T.(Nakagome,Y.): "A novel nonsense mutation in the HMG box of the SRY gene in a patient with XY sex reversal" Human Molec.Genet.3. 1437-1438 (1994)
[Publications] Lin,S.(Nakagome,Y.): "A Y-associated allele is shared among a few ethnic groups is Asia" Jpn.J.Hum.Genet.39. 299-304 (1994)
[Publications] Tsutsumi,O.(Nakagome,Y.): "Intact sex determining region Y(SRY)in a patient with XY pure gonadal dysgenesis and a twin brother" Endocrine J.41. 281-285 (1994)
[Publications] Yokota,Y.(Nakagome,Y.): "Monozygotic twins of different apparent sex" Amer.J.Med.Genet.53. 52-55 (1994)
[Publications] Nakamura,Y.: "Isolation and mapping of 328 new cosmid markers on human chromosome 8:construction of a high resolution cytogenetic map of chromosome 8 with 416 markers" Cytogenet.Cell Genet.65. 115-118 (1994)
[Publications] Furukawa,Y.(Nakamura,Y.): "Structure,expression and chromosomal assignment of the human α-catenin gene" Cytogenet.Cell Genet.65. 74-78 (1994)
[Publications] Fujiwara,Y.(Nakamura,Y): "A 3-Mb physical map of the region at chromosome 8p21,3-p22,including a 600-kb region commonly deleted in human hepatocellular carcinoma,colorectal cancer,and non-small cell lung cancer" Genes Chromo.Camcer. 10. 7-14 (1994)
[Publications] Koyama,K.(Nakamura,Y.): "Mapping of the human nicotinic acetylcholine receptor(nAChR)beta-3 gene within Chromosome 8p11.2" Genomics. 21. 460-461 (1994)
[Publications] Arakawa,H.(Nakamura,Y.): "Molecular Cloning and expression of a novel human gene that is highly homologous to human FK-506-binding protein 12KD(hFKBP-12),and characterization of two alternativelyspliced transcripts" Biochem.Biophy Res.Com.200. 836-843 (1994)
[Publications] Katagiri,K.(Nakamura,Y.): "Human metalloprotease/disintegrin-like(MDC)gene:Exon-intron organization and alternative splicing" Cytogenet.Cell Genet.,. (in peess).
[Publications] Tanaka,T.(Imai,T.,Nakamura,Y.): "Genetic linkage analyses of Romano-ward syndrome(RWS)in 13 Japanese families" Human Genet.94. 380-384 (1994)
[Publications] Nakatsuru,S.(Nakamura,Y.): "Molecular cloning of a novel human cDNA homologous to CDC10 in Saccharomyces cerevisiae" Biochem.Biophy.Res.Com. 202. 82-87 (1994)
[Publications] Nakatsuru,S.(Nakamura,Y.): "Isolation and mapping of a novel human gene encoding a product homologous to yeast proteins involving in DNA replication" Cytogenet.Cell Genet.68. 226-230 (1994)
[Publications] Tanaka,T.(Nakamura,Y.): "Assignment of the human caltractin gene to X_q28 by fluorescencein situ hybridization" Genomics,. (in press).
[Publications] Toda,T.(Nakamura,Y.): "Refined mapping of a gene responsible for Fukuyama type congenital muscular dystrophy;evidence for a strong linkage disequilibrium" Am.J.Hum.Genet.55. 946-950 (1994)
[Publications] Ohata,H.(Nakamura,Y.): "Mapping of the human autoantigen pericentriol material 1(PCM-1)gene to chromosome 8p21.3-p22" Genomics. 24. 404-406 (1994)
[Publications] Sudo,K.(Nakamura,Y.): "2,058 expressed sequence tags(ESTs)from a human fetal lung cDNA library" Genomics. 24. 276-279 (1994)
[Publications] Arakawa,H.(Nakamura,Y.): "Molecular cloning,characterization,and chromosomal mapping of a novel human gene that is highly homologous to Xenopus transcription factor IIIA" Cytogenet.Cell Genet.,. (in press).
[Publications] Ikegawa,S.(Nakamura,Y.): "Isolation,characterization and chromosomal assignment of human colligin2" Cytogenet.Cell Genet.,. (in press).
[Publications] Ikegawa,S.(Nakamura,Y.): "Mutations of the fibroblast growth factor-3 gene in one familial and six sporadic cases of Japanese patients with achondroplasia" Human Genet.,. (in press).
[Publications] Fujiwara,Y.(Nakamura,Y.): "Isolation of a candidate tumor suppressor gene on chromosome 8p21.3-p22 that is homologous to an extracellular domain of the PDGF receptor data gene" Oncogene,. (in press).
[Publications] Koyama,K.(Nakamura,Y.): "Isolation of 115 human chromosome 8-specific expressed-sequence tags by exon amplification" Genomics,. (in press).
[Publications] Yoshiura,K.(Inazawa,J.,Nakamura,Y.,Niikawa,N.): "Mapping of the 8q translocation breakpoint of t(8;13)observed in a patient with multiple exostoses" Genes Chrom.Cancer. 9. 57-61 (1994)
[Publications] Kishino,T.(Niikawa,N.): "Assignment of the human moesin gene(MSN)to chromosomal region Xq11.2-q12" Cytogenet.Cell Genet.66. 167-169 (1994)
[Publications] Kubota,T.(Niikawa,N.): "GABA receptor beta 3 subunit gene is possibly paternally imprinted in humans" Am.J.Med.Genet.49. 452-453 (1994)
[Publications] Sawai,N.(Niikawa,N.): "Case of 46,XX/47,XY,+21 chimerism in a newborn infant with ambiguous genitalia" Am.J.Med.Genet.49. 428-430 (1994)
[Publications] Jinno,Y.(Niikawa,N.): "Mosaic and polymorphic imprinting of the WT1 gene in humans" Nature Genet.6. 305-309 (1994)
[Publications] Soejima,H.(Nakamura,Y.,Niikawa,N.): "Fifty novel sequence-tagged sites(STSs)on a human chromosomal region,11q13.4-q25,identified from microclones generated by microdissection" Cytogenet.Cell Genet.(in press).
[Publications] Tamura,T.(Niikawa,N.): "Assignment of the human PAX4 to chromosome 7q32 by fluorescence in situhybridization" Cytogenet.Cell Genet.,. 66. 132-134 (1994)
[Publications] Kubota,T.(Niikawa,N.): "Excess functional copy of allele at chromosomal region 11-15 may cause Wiedemann-Beckwith syndrome" Am.J.Med.Genet.49. 378-383 (1994)
[Publications] Tsukamoto,K.(Nakamura,Y.,Niikawa,N.): "Isolation of two isoforms of the PAX3 gene transcripts and their tissuespecific alternative expression in human adult tissues" Hum.Genet.93. 270-274 (1994)
[Publications] Matsumoto,N.(Niikawa,N.): "Confirmation of Down syndrome critical region by FISH analysis in a patient with 21p+" Am.J.Med.Genet.,. (in press).
[Publications] Matsumoto,N.(Niikawa,N.): "DNA-based prenatal diagnosis for group A xeroderma pigmentosum in a chorionic villus sample" Prenatal Diagnosis,. (in press).
[Publications] Sachse,R.(Hayashi,K.): "DNA aberrations at the retinoblastoma gene locus in human squamous cell carcinomas of the lung" Oncogene. 9. 39-47 (1994)
[Publications] Yashima,K.(Hayashi,K.): "Mutations of the adenomatous polyposis coli gene in the mutation cluster region:Comparison of human pancreatic and colorectal cancer" Int.J.Cancer. 59. 43-47 (1994)
[Publications] Furihata,C.(Hayashi,K.): "Induction of c-fos and c-myc oncogene in the pyrolic mucosa of rat stomach by N-methy1-N'-nitro-N-nitrosoguanidine and taurocholate" Cancer Letters. 83. 215-220 (1994)
[Publications] Genji,T.(Hayashi,K.): "Specific detection of positive and negative stranded hepatitis C viral RNA using chemical RNA modification" Arch.Virol.134. 293-302 (1994)
[Publications] Iizuka,M.(Hayashi,K.): "Mapping of 28(CA)n microsatellite repeats and 13 Alu markers on human chromsome 11 using a panel of somatic cell hybrids" Genomics. 19. 581-584 (1994)
[Publications] Munakata,N.(Hayashi,K.): "Molecular characterization of thirteen gyrA mutations conferring nalidixic acid resistance in Bacillus subtilis" Mol.Gen.Genet.244. 97-103 (1994)
[Publications] Makino,R.(Hayashi,K.): "Cloning and characterization of a c-myc intron binding protein(MIBP1)" Nucleic Acids Res.,. (in press).
[Publications] Kawamura,M.(Hayashi,K.): "Mutations of the p53 and ras genes in childhood t(1:19)-acute lymphoblastic leukemia" Blood,. (in press).
[Publications] Sugano,K.(Hayashi,K.): "Single-strand conformation polymorphism analysis by perpendicular temperature-gradient gel electrophoresis" Electrophoresis,. (in press).
[Publications] Iizuka,M.(Hayashi,K.): "Twelve simple repeat sequence polymorphisms on chromosome 11,4,16 and 21" Hum.Molec.Genet.,. (submitted).
[Publications] Sugano,K.(Hayashi,K.): "Sensitive and specific detection of K-ras codon 12 mutations in pancreatic juice for diagnosis of pancreatic neoplasms by non-radioisotopic single-strand conformation polymorphism analysis" Cancer Res.,. (submitted).
[Publications] Yoshikawa,H.(Matsubara,K,Fujiyama,A.): "Chromosome-specific assignment of human genomic not 1 restriction fragments on two-dimensional electrophoresis profile" Nature Genet.,. (in preparation).
[Publications] Sugano,K.(Hayashi,K.): "Sensitive and specific detection of K-ras codon 12 mutations in pancreatic juice for diagnosis of pancreatic neoplasms by non-radioisotopic single-strand conformation polymorphism analysis" Cancer Res.,. (submitted).
[Publications] Yoshikawa,H.(Matsubara,K,Fujiyama,A.): "Chromosome-specific assignment of human genomic not 1 restriction fragments on two-dimensional electrophoresis profile" Nature Genet.,. (in preparation).
[Publications] Seki,N.(Masumoto,H.): "Mapping of the human centromere protein B gene(CENPB)to chromosome 20p13 by fluorescence in situ hybridization" Genomics. 24. 187-188 (1994)
[Publications] Ikeno,M.(Masumoto,H.): "Distribution of CENP-B boxes reflected in CREST centromere antigenic sites on longrange α-satellite DNA arrays of human chromosome 21" Human Mol.Genet.3. 1245-1257 (1994)
[Publications] Haino,M.(Matsuda,F.): "Comparison and evolution of human immunoglobulin V_H segments located in the 3'0.8-megabase region:evidence for unidirectional transfer of segmental gene sequences" J.Biol.Chem.269. 2619-2626 (1994)
[Publications] Shin,E.K.(Matsuda,F.): "Variable-regions of immunoglobulin heavy-chain genes encoding anti-thhyrotropin(TSH)receptor antibodies of patients with Graves'disease" J.Immunol.152. 1485-1492 (1994)
[Publications] Taniwaki,M.(Matsuda,F.): "Detection of 14q32 translocations in B-cell malignancies by in situ hybridization with yeast artificial chromosome clones containing the human IgH gene locus" Blood. 83. 2962-2969 (1994)
[Publications] Nagaoka,H.(Imai,T.,Matsuda,F.): "Recent translocation of variable and diversity (D) segment of the human immunoglobulin heavy chain from chromosome 14 to chromsomes 15 and 16" Genomics. 22. 189-197 (1994)
[Publications] Nakamura,N.(Matsuda,F.): "Heterogeneity and diversity of IgM and IgG lupus anticoagulants in an individual with systemic lupus erythematosus" Biochem.Biophys.Res.Commun. 203. 1789-1794 (1994)
[Publications] 磯部正治: "細胞工学別冊FISH実験プロトコールヒト・ゲノム解析から染色体・遺伝子診断まで(松原謙一、吉川寛監修) FISH法に用いるプローブの種類と標識法" 秀潤社, 58-71 (1994)
[Publications] 高田肇(猪子英俊): "「外科臨床ハンドブック」外科臨床の分子生物学(出月康夫、北島政樹編) 臓器移植と分子生物学-拒絶反応とアロ抗原認識の分子機構" 中山書店, 251-266 (1994)
[Publications] Kimura,A: "Developmental Mechanisms of heart Disease(Clark EB,Markwald PR,eds.)Genetic analysis of hypertrophic cardiomyopathy in Japan" Futura Press, 113-120 (1995)
[Publications] 平井百樹: "細胞工学別冊FISH実験プロトコールヒト・ゲノム解析から染色体・遺伝子診断まで(松原謙一、吉川寛監修) 染色体ペインティング FISH法の染色体画像処理と解析" 秀潤社, 108-112 168-174 (1994 1994)
[Publications] Matsuda,F.: "“Immunoglobulin Genes"(T.Hojo and F.W.Alt,eds.) Immunoglobulin heavy chain loci of mouse and man" Academic Press,London(in press), (1995)
[Publications] 阿部達生: "遺伝子と日常の病気" 医学書院, 358 (1994)
[Publications] 奥村克純: "細胞工学別冊FISH実験プロトコールヒト・ゲノム解析から染色体・遺伝子診断まで(松原謙一、吉川寛監修) 第2部第10章 YACを含むトータルYeast DNAをプローブとするFISH法第2部第12章冷却CCDカメラを用いたFISH法第2部第14章 DNAファイバーを用いた近接クローンの解析第3部第7章 FISH法を用いたDNA複製時期の判定" 秀潤社, 124-127, 137-142, 151-154, 211-215 (1994 1994 1994 1994)
[Publications] Okumura,K.: "Animal Cell Technology:Basic & Applied Aspects,Vol.6" Kluwer Academic Publishers,Netherlands, 1-615 (1994)

1994 Fiscal Year Final Research Report Summary

ヒト・ゲノム解析研究

Principal Investigator

松原 謙一 大阪大学, 細胞生体工学センター, 教授 (20037394)

Research Products

[Publications] Matsuo,A.(Isobe,M.): "Immunohistochemical localization in the rat brain of an epitope corresponding to the fibroblast growth factor receptor-1" Neuroscience. 60. 49-66 (1994)

[Publications] Isobe,M.: "Chromosomal localization of an SH2 containing tyrosine phosphatase (SH-PTP)gene to chromosome 12q24.1" Oncogene. 9. 1751-1753 (1994)

[Publications] Isobe,M.: "Assignment of the true and processed genes for human glycine decarboxylase to 9p23-24 and 4q12" Biochem.Biophys.Res.Comm.203. 1483-1487 (1994)

[Publications] Virgilio,L.(Isobe,M.,Imai,T.,Ikemura,T.): "Identification of the lymphocyte-specific tcl-1 gene involved in T cell malignancies" Proc.Natl.Acad.Sci.USA,. (in press).

[Publications] Ando,A.(Inoko,H.): "Cloning of a new kinesin-related gene located at the centromeric end of the human MHC region" Immunogenetics. 39. 194-200 (1994)

[Publications] Koshizaka,T.(Inoko,H.): "A new HLA-DPB1 allel,DPB1*SUT(DPB1*4701)" Tissue Antigens. 43. 50-53 (1994)

[Publications] Inoko,H.: "Role of human leukocyte antigens and T lymphocytes and in the immune response" Annals of Cancer Research and Therapy. 2. 173-191 (1994)

[Publications] Shindo,Y.(Inoko,H.): "HLA-DRB1 typing of Vogt-Koyanagi-Harada's disease by PCR-RFLP and the strong association with DRB1*045 and DRB1*0410" British Opthal. 78. 223-226 (1994)

[Publications] Shindo,Y.(Inoko,H.): "Complete association of the HLA DRB1*4 and DQB1*04 alleles with Vogt-Koyanagi-Harada's disease" Hum.Immunol.39. 169-176 (1994)

[Publications] Onuma,H.(Inoko,H.): "Association of HLA-DRB1*0501 with early-onset Graves'disease in Japanese" Hum.Immunol.39. 195-201 (1994)

[Publications] Ishihara,M.(Inoko,H.): "Molecular genetic studies of HLA class II alleles in sarcoidosios" Tissue Antigens. 43. 238-241 (1994)

[Publications] Ogawa,K.(Inoko,H.): "A novel HLA-DPB1 allele,DPB1*3601(DPB1*KT)" Tissue Antigens. 44. 134-136 (1994)

[Publications] 成瀬妙子(猪子英俊): "DNAによるHLAタイピングの基礎(1)HLA領域を構成する遺伝子その塩基配列" Organ Biology. 1. 95-102 (1994)

[Publications] Toda,T.(Imai,T.): "Isolation and characterization of a novel gene encoding nuclear protein at a locus(D11S636)tightly linked to multiple endocrine neoplasia type 1(MEN1)" Hum.Molec.Genet.3. 465-470 (1994)

[Publications] Imai,T.: "Assignment of the human ribosomal protein S25 gene(RPS25)to chromosome 11q23.3 by sequence analysis of the marker D11S456" Genomics. 20. 142-143 (1994)

[Publications] Michael R.James(Imai,T.,Nakamura,Y.): "A radiation hybrid map of 506 STS markers spanning human chromosome II" Nature genetics. 8. 70-76 (1994)

[Publications] Iida,A.(Imai,T.,Nakamura,Y.): "Allelic losses on chromosome 11q13 in aldosterone-producing adrenal tumors" Genes Chromo.Cancer. (in press).

[Publications] Mitani,K.(Ohki,M.): "Generation of the AMLI-EVII fusion gene in the t(3;21)(q26:q22)causes blastic crisis in chronic myelocytic leukemia" EMBO J.13. 504-510 (1994)

[Publications] Ichikawa,H.(Ohki,M.): "An RNA-binding protein gene,TLS/FUS,is fused to ERG in human myeloid leukemia with t(16:21)chromosomal translocation" Cancer Res.54. 2865-2868 (1994)

[Publications] Takeda,O.(Kaneko,Y.): "There may be two tumor suppressor genes on chromosome arm 1p closely as sociated with biologically distinctsubtypes of neuroblastoma" Genes Chromo.Cancer. 10. 30-39 (1994)

[Publications] Nishi,H.(Kimura,A.): "Possible gene dose effect of a mutant cardiac β-myosin heavy chain gene on the clinical expression of familial hypertrophic cardiomyopathy" Biochem.Biophys.Res.Commun.200. 549-556 (1994)

[Publications] Kuwano,A.(Kondo,I.): "Precise chromosomal locations of DRPLA,F8vWF and PTHLA in human chromosome 12p" Hum.Genet.,. (in press).

[Publications] Tanahashi,H.(Sakaki,Y.): "Sixty news STSs(Sequence-Tagged Sites)of human chromosome21" DNA Res.1. 85-89 (1994)

[Publications] Zhao,N.(Sakaki,Y.): "Cloning and sequence analysis of the human SNAP25cDNA" Gene. 145. 313-314 (1994)

[Publications] Suzuki,H.(Sakaki,Y.): "Genomic analysis of a NF1-related psendogene on human chromosome 21" Gene. 147. 277-280 (1994)

[Publications] Ito,T.(Sakaki,Y.): "Flourescent differential display:arbitrarily primed RT-PCR fingerprinting on an automnated DNA sequencer" FEBS Letter. 351. 231-236 (1994)

[Publications] Zhao,N.(Sakaki,Y.): "High-density cDNA filter analysis:a novel approach for large-scale,quantitative analysis of gene expression" Gene,. (in press).

[Publications] Shimizu,N.: "Detection of the EGF receptor gene amplification in the human squamous cell carcinomas using FISH technique" Jpn.J.Cancer Res.85(6). 567-571 (1994)

[Publications] Wang,Y.(Shimizu,N.): "The human genes for desmogleins(DSG1 and DSG3)are located in a small region on chromosome 18q12" Genomics. 20(3). 492-495 (1994)

[Publications] Fujita,N.(Shimizu,N.): "Genetic origin analysis of hydatidiform mole and non-molar abortion using the polymerase chain reaction method" Acta Obstet.Gynecol.Scand.73(4). 719-725 (1994)

[Publications] Minoshima,S.(Shimizu,N.): "Mapping of the gene for human xanthine dehydrogenase (oxidase)(XDH)to the band p23 of chromosome 2" Cytogenet.Cell Genet.68(1-2). 52-53 (1995)

[Publications] Amagai,M.(Shimizu,N.): "Assignment of the human genes for desmocollin 3 and desmocollin 4 to chromosome 18q12" Genomics. (in press).

[Publications] Sakai,K.(Shimizu,N.): "Human rebosomal RNA gene cluster:Identification of the proximal end containing a novel tandem repeat sequence" Genomics. (in press).

[Publications] Kubota,R.(Shimizu,N.): "Mapping of the human gene for Pmel 17(D12S53E)to chromosome 12q13-q14" Genomics. (in press).

[Publications] Kudoh,J.(Shimizu,N.): "Localization of the human desmoyokin/AHNAK gene to chromosome 11q12 by somatic cell hybrid analysis and flourescence in situ hybridization" Cytogenet.Cell Genet.,in press.

[Publications] Nakano,R.(Tsuji,S.): "A novel mutation in Cu/Zn superoxide dismutase gene in Japanese familial amyotrophic lateral sclerosis" Biochem.Biophys.Res.Commun.200(2). 695-703 (1994)

[Publications] Okuizumi,K.(Tsuji,S.): "ApoE-_ε4 and early-onset Alzheimer's" Nature Genet.7(1). 10-11 (1994)

[Publications] Takano,H.(Tsuji,S.): "Effect of intravenous immunoglobulin in Lambert-Eaton myasthenic syndrome with small-cell lung cancer:Correlation with the titer of anti-voltage-gated calcium channel antibody" Muscle Nerve. 17. 1073-1075 (1994)

[Publications] Koide,R.(Tsuji,S.): "Hereditary progressive dystonia with marked diurnal fluctuation caused by mutations in the GTP cyclohydrolase I gene" Nature Genet.8. 236-242 (1994)

[Publications] Sequeiros,J.(Tsuji,S.): "Genetic linkage studies of Machado-Joseph disease with chromosome 14q STRPs in 16 Portuguese-Azorean Kindreds" Genomics. 21. 645-648 (1994)

[Publications] Fujigasaki,H.(Tsuji,S.): "Mutational analysis of the amyloid precursor protein gene in Japanese familial Alzheimer's disease kindreds" Hum.Genet.93. 460-462 (1994)

[Publications] Naruse,S(Tsuji,S): "Structures of the human and mouse growth inhibitory factor-encoding genes" Gene. 144. 283-287 (1994)

[Publications] Ichinose,H.(Tsuji,S.): "Unstable expansion of CAG repeat in hereditary dentatorubral-pallidoluysian atrophy(DRPLA)" Nature Genet.6(1). 9-13 (1994)

[Publications] Kobayashi,K.(Nakagome,Y.): "PCR analysis of the Y chromosome long arm in azoospermic patients:evidence for a second locus required for spermatogenesis" Human Molec.Genet.3. 1965-1967 (1994)

[Publications] Nakahori,Y.(Nakagome,Y.): "A locus of the candidate gene family for azoospermia factor(YRRM2)is polymorphic with a null allele in Japanese males" Human Molec.Genet.3. 1710 (1994)

[Publications] Iida,T.(Nakagome,Y.): "A novel nonsense mutation in the HMG box of the SRY gene in a patient with XY sex reversal" Human Molec.Genet.3. 1437-1438 (1994)

[Publications] Lin,S.(Nakagome,Y.): "A Y-associated allele is shared among a few ethnic groups is Asia" Jpn.J.Hum.Genet.39. 299-304 (1994)

[Publications] Tsutsumi,O.(Nakagome,Y.): "Intact sex determining region Y(SRY)in a patient with XY pure gonadal dysgenesis and a twin brother" Endocrine J.41. 281-285 (1994)

[Publications] Yokota,Y.(Nakagome,Y.): "Monozygotic twins of different apparent sex" Amer.J.Med.Genet.53. 52-55 (1994)

[Publications] Nakamura,Y.: "Isolation and mapping of 328 new cosmid markers on human chromosome 8:construction of a high resolution cytogenetic map of chromosome 8 with 416 markers" Cytogenet.Cell Genet.65. 115-118 (1994)

[Publications] Furukawa,Y.(Nakamura,Y.): "Structure,expression and chromosomal assignment of the human α-catenin gene" Cytogenet.Cell Genet.65. 74-78 (1994)

[Publications] Fujiwara,Y.(Nakamura,Y): "A 3-Mb physical map of the region at chromosome 8p21,3-p22,including a 600-kb region commonly deleted in human hepatocellular carcinoma,colorectal cancer,and non-small cell lung cancer" Genes Chromo.Camcer. 10. 7-14 (1994)

[Publications] Koyama,K.(Nakamura,Y.): "Mapping of the human nicotinic acetylcholine receptor(nAChR)beta-3 gene within Chromosome 8p11.2" Genomics. 21. 460-461 (1994)

[Publications] Arakawa,H.(Nakamura,Y.): "Molecular Cloning and expression of a novel human gene that is highly homologous to human FK-506-binding protein 12KD(hFKBP-12),and characterization of two alternativelyspliced transcripts" Biochem.Biophy Res.Com.200. 836-843 (1994)

[Publications] Katagiri,K.(Nakamura,Y.): "Human metalloprotease/disintegrin-like(MDC)gene:Exon-intron organization and alternative splicing" Cytogenet.Cell Genet.,. (in peess).

[Publications] Tanaka,T.(Imai,T.,Nakamura,Y.): "Genetic linkage analyses of Romano-ward syndrome(RWS)in 13 Japanese families" Human Genet.94. 380-384 (1994)

[Publications] Nakatsuru,S.(Nakamura,Y.): "Molecular cloning of a novel human cDNA homologous to CDC10 in Saccharomyces cerevisiae" Biochem.Biophy.Res.Com. 202. 82-87 (1994)

[Publications] Nakatsuru,S.(Nakamura,Y.): "Isolation and mapping of a novel human gene encoding a product homologous to yeast proteins involving in DNA replication" Cytogenet.Cell Genet.68. 226-230 (1994)

[Publications] Tanaka,T.(Nakamura,Y.): "Assignment of the human caltractin gene to X_q28 by fluorescencein situ hybridization" Genomics,. (in press).

[Publications] Toda,T.(Nakamura,Y.): "Refined mapping of a gene responsible for Fukuyama type congenital muscular dystrophy;evidence for a strong linkage disequilibrium" Am.J.Hum.Genet.55. 946-950 (1994)

[Publications] Ohata,H.(Nakamura,Y.): "Mapping of the human autoantigen pericentriol material 1(PCM-1)gene to chromosome 8p21.3-p22" Genomics. 24. 404-406 (1994)

[Publications] Sudo,K.(Nakamura,Y.): "2,058 expressed sequence tags(ESTs)from a human fetal lung cDNA library" Genomics. 24. 276-279 (1994)

[Publications] Arakawa,H.(Nakamura,Y.): "Molecular cloning,characterization,and chromosomal mapping of a novel human gene that is highly homologous to Xenopus transcription factor IIIA" Cytogenet.Cell Genet.,. (in press).

[Publications] Ikegawa,S.(Nakamura,Y.): "Isolation,characterization and chromosomal assignment of human colligin2" Cytogenet.Cell Genet.,. (in press).

[Publications] Ikegawa,S.(Nakamura,Y.): "Mutations of the fibroblast growth factor-3 gene in one familial and six sporadic cases of Japanese patients with achondroplasia" Human Genet.,. (in press).

[Publications] Fujiwara,Y.(Nakamura,Y.): "Isolation of a candidate tumor suppressor gene on chromosome 8p21.3-p22 that is homologous to an extracellular domain of the PDGF receptor data gene" Oncogene,. (in press).

[Publications] Koyama,K.(Nakamura,Y.): "Isolation of 115 human chromosome 8-specific expressed-sequence tags by exon amplification" Genomics,. (in press).

[Publications] Yoshiura,K.(Inazawa,J.,Nakamura,Y.,Niikawa,N.): "Mapping of the 8q translocation breakpoint of t(8;13)observed in a patient with multiple exostoses" Genes Chrom.Cancer. 9. 57-61 (1994)

[Publications] Kishino,T.(Niikawa,N.): "Assignment of the human moesin gene(MSN)to chromosomal region Xq11.2-q12" Cytogenet.Cell Genet.66. 167-169 (1994)

[Publications] Kubota,T.(Niikawa,N.): "GABA receptor beta 3 subunit gene is possibly paternally imprinted in humans" Am.J.Med.Genet.49. 452-453 (1994)

[Publications] Sawai,N.(Niikawa,N.): "Case of 46,XX/47,XY,+21 chimerism in a newborn infant with ambiguous genitalia" Am.J.Med.Genet.49. 428-430 (1994)

[Publications] Jinno,Y.(Niikawa,N.): "Mosaic and polymorphic imprinting of the WT1 gene in humans" Nature Genet.6. 305-309 (1994)

[Publications] Soejima,H.(Nakamura,Y.,Niikawa,N.): "Fifty novel sequence-tagged sites(STSs)on a human chromosomal region,11q13.4-q25,identified from microclones generated by microdissection" Cytogenet.Cell Genet.(in press).

[Publications] Tamura,T.(Niikawa,N.): "Assignment of the human PAX4 to chromosome 7q32 by fluorescence in situhybridization" Cytogenet.Cell Genet.,. 66. 132-134 (1994)

[Publications] Kubota,T.(Niikawa,N.): "Excess functional copy of allele at chromosomal region 11-15 may cause Wiedemann-Beckwith syndrome" Am.J.Med.Genet.49. 378-383 (1994)

松原謙一大阪大学, 細胞生体工学センター, 教授 (20037394)

[Publications] Koshizaka,T.(Inoko,H.): "A new HLA-DPB1 allel,DPB1SUT(DPB14701)" Tissue Antigens. 43. 50-53 (1994)

[Publications] Shindo,Y.(Inoko,H.): "HLA-DRB1 typing of Vogt-Koyanagi-Harada's disease by PCR-RFLP and the strong association with DRB1045 and DRB10410" British Opthal. 78. 223-226 (1994)

[Publications] Shindo,Y.(Inoko,H.): "Complete association of the HLA DRB14 and DQB104 alleles with Vogt-Koyanagi-Harada's disease" Hum.Immunol.39. 169-176 (1994)

[Publications] Ogawa,K.(Inoko,H.): "A novel HLA-DPB1 allele,DPB13601(DPB1KT)" Tissue Antigens. 44. 134-136 (1994)