1995 Fiscal Year Annual Research Report

レドックス制御とシグナル伝達機構

Research Project

Project/Area Number	07041160
Research Institution	Kyoto University
Principal Investigator	淀井清京都大学, ウイルス研究所, 教授 (80108993)
Co-Investigator(Kenkyū-buntansha)	コルスマイヤースタンレワシントン大学, 病理学, 教授カリンマイケルカリフォルニア大学, サンディエゴ校・医学部・薬理学, 準教授チュルツトマスグスタブルーシー研究所, 内科, 教授キンユン米国NIH, 研究員キャンビアジョンナショナルジューイッシュセンター, 免疫・呼吸器医学基礎科学部, 部長口野嘉幸国立がんセンター研究所, 生物物理部, 教授 (60124418) 豊国伸哉京都大学, 医学部, 講師 (90252460) 花房秀三郎ロックフェラー大学, 分子腫瘍学, 教授山本健一金沢大学がん研究所, 病態生理部, 教授 (60115285) 佐邊壽孝京都大学, ウィルス研究所, 助教授 (40187282)
Keywords	胚形成 / ノックアウトマウス / 白血病 / 酸化ストレス / オキシゲン、バースト / プロモーター解析 / レドックス制御 / チオレドキシン
Research Abstract	7年度は、1)ADF結合蛋白質の単離、およびその機能解析、2)ADF遺伝子発現調節部位結合因子の同定、単離、3)ADF欠損マウスを作製し、これを用いて、個体レベル、もしくは種々の組織から細胞単離し、正常及びADF-transgenic mouceとの比較において、ADFの抗酸化機能について解析を行うこと、4)核内において、及び、NF-kB,thioredoxin/ADFなどの蛋白性因子の還元状態保持に寄与する物質の単離、同定する事などを目指し、その為に必要な研究交流・情報交換を行うことが目的であった。 7年度においては、各プロジェクトは総体的に、順調に進んだ。具体的には、1)ADF遺伝子プロモーター部位のなかで、酸化ストレスに応答する領域の特定を行った、2)ADF欠損マウスの作成に成功した、3)ADF結合性蛋白質のcDNA単離が既に進行し、幾つかの候補cDNAを得た、等が挙げられる。特に、同定したADF遺伝子プロモーターの酸化ストレス応答領域の核酸配列は、他の既知の制御領域配列とは異なっており、新しい制御因子の関与が示唆され、ADF結合性蛋白質のcDNA単離の結果、従来ある程度予想はしていたが、ADFがマクロファージなどのoxygen burst過程に関与している可能性が示唆された。

Research Products

(17 results)

All Other

All Publications (17 results)

[Publications] K,Furuke: "FK506 and cyclosporin A: a new medranism of immune modulation via redox control" International Immunology. 7. 985-993 (1995)
[Publications] N,Sato: "Thiol compounds and adult T-cell Ieukemia virus infection: A potential therapeutic approach" Methods in Ensymology,Biothiol. 252. 343-348 (1995)
[Publications] M,Matsui: "Structure of mouse thioredoxin encoding gene and its processed pseudogene" Gene. 152. 165-171 (1995)
[Publications] Y,Sachi: "Induction of ADF/TRX by oxdative stress in keratinocytes and lymphoid cells" Immunology Letter. 44. 189-193 (1995)
[Publications] Y,U-Taniguchi: "Cell cycle inhibition of HTLV-I transfoemrd Tcell lines by retinoic acid: the possible therpeutic use of thioredOxin reductase inhibitors" Oncology Research. 7. 183-189 (1995)
[Publications] N,Sato: "Thiol-: mediated redox regulation of apoptosis Possible roles of cellular thiols Other-then gluitathione in T cell apoptosis" Journal of Immunology. 154. 3194-3203 (1995)
[Publications] K,Sugie: "Activation of natural Killer cellsby the mAb YTA-1 that recoghnzes leukocyte function-associated antigen-1" Int. Immunol.7. 763-769 (1995)
[Publications] H,Koyanagi: "Role of ADF/TRX and its inhibitor on The release of major basic protein from human eosinophils" Biochemical and Biophysical Research Communications. 213. 1140-1147 (1995)
[Publications] H,Wada: "Rejection and expression of thioredoxin in trasplanTed canine lung" CHEST. 108. 810-814 (1995)
[Publications] T,Sasada: "Redox Control of Resistance To cis-Diamminedichloroplatinum (II) (CDDP) : Protective Effect of Human Thioredoxin against CDDP-Induced Cytotoxicity" Journal of Clinical Immunology. (in press). (1996)
[Publications] K,Okamoto: "Overexpression of human mut T homo logue gene messenger RNA in rehal cell carcinoma: evidence for persistent oxdative stress in cancer" Int. J. Caner. (in press). (1996)
[Publications] S, Toyokuni: "Hypothesis: Persistent oxidative stress in cancer" FEBS Lett.358. 1-3 (1995)
[Publications] S. Toyokuni: "The monoclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct" FEBS Lett.359. 189-191 (1995)
[Publications] Sun X.: "Identification of a novel p53 promoter cis-element involved in genotoxic stress-inducible p53 gene exrpession" Mol,Cell Biol.15. 4489-4496 (1995)
[Publications] Jun Qin: "Solution structure of human thioredoxinin mind disulfide intermediate complex with its Target peptide from the transcription factor NFKB" Structure. 3. 289-297 (1995)
[Publications] H,Sabe: "Possible v-Crk-induced Transformation Thrcugh Activation of Src Kinases" Journal of Bidogical Chemistry. 270. 31219-31224 (1995)
[Publications] J,Yodoi and T,Udiyama: "Adult T Cell Leukemia and Related Diseases Medical Intelligence Unit" R. G,Landes Company,Bicmedical Publishers Austin,Springer-Verlag, 139 (1995)

1995 Fiscal Year Annual Research Report

レドックス制御とシグナル伝達機構

Principal Investigator

淀井 清 京都大学, ウイルス研究所, 教授 (80108993)

Research Products

[Publications] K,Furuke: "FK506 and cyclosporin A: a new medranism of immune modulation via redox control" International Immunology. 7. 985-993 (1995)

[Publications] N,Sato: "Thiol compounds and adult T-cell Ieukemia virus infection: A potential therapeutic approach" Methods in Ensymology,Biothiol. 252. 343-348 (1995)

[Publications] M,Matsui: "Structure of mouse thioredoxin encoding gene and its processed pseudogene" Gene. 152. 165-171 (1995)

[Publications] Y,Sachi: "Induction of ADF/TRX by oxdative stress in keratinocytes and lymphoid cells" Immunology Letter. 44. 189-193 (1995)

[Publications] Y,U-Taniguchi: "Cell cycle inhibition of HTLV-I transfoemrd Tcell lines by retinoic acid: the possible therpeutic use of thioredOxin reductase inhibitors" Oncology Research. 7. 183-189 (1995)

[Publications] N,Sato: "Thiol-: mediated redox regulation of apoptosis Possible roles of cellular thiols Other-then gluitathione in T cell apoptosis" Journal of Immunology. 154. 3194-3203 (1995)

[Publications] K,Sugie: "Activation of natural Killer cellsby the mAb YTA-1 that recoghnzes leukocyte function-associated antigen-1" Int. Immunol.7. 763-769 (1995)

[Publications] H,Koyanagi: "Role of ADF/TRX and its inhibitor on The release of major basic protein from human eosinophils" Biochemical and Biophysical Research Communications. 213. 1140-1147 (1995)

[Publications] H,Wada: "Rejection and expression of thioredoxin in trasplanTed canine lung" CHEST. 108. 810-814 (1995)

[Publications] T,Sasada: "Redox Control of Resistance To cis-Diamminedichloroplatinum (II) (CDDP) : Protective Effect of Human Thioredoxin against CDDP-Induced Cytotoxicity" Journal of Clinical Immunology. (in press). (1996)

[Publications] K,Okamoto: "Overexpression of human mut T homo logue gene messenger RNA in rehal cell carcinoma: evidence for persistent oxdative stress in cancer" Int. J. Caner. (in press). (1996)

[Publications] S, Toyokuni: "Hypothesis: Persistent oxidative stress in cancer" FEBS Lett.358. 1-3 (1995)

[Publications] S. Toyokuni: "The monoclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct" FEBS Lett.359. 189-191 (1995)

[Publications] Sun X.: "Identification of a novel p53 promoter cis-element involved in genotoxic stress-inducible p53 gene exrpession" Mol,Cell Biol.15. 4489-4496 (1995)

[Publications] Jun Qin: "Solution structure of human thioredoxinin mind disulfide intermediate complex with its Target peptide from the transcription factor NFKB" Structure. 3. 289-297 (1995)

[Publications] H,Sabe: "Possible v-Crk-induced Transformation Thrcugh Activation of Src Kinases" Journal of Bidogical Chemistry. 270. 31219-31224 (1995)

[Publications] J,Yodoi and T,Udiyama: "Adult T Cell Leukemia and Related Diseases Medical Intelligence Unit" R. G,Landes Company,Bicmedical Publishers Austin,Springer-Verlag, 139 (1995)

淀井清京都大学, ウイルス研究所, 教授 (80108993)