1995 Fiscal Year Annual Research Report

南米先住民族の人類遺伝学的研究

Research Project

Project/Area Number	07041171
Research Institution	Aichi Cancer Center Research Institute
Principal Investigator	田島和雄愛知県がんセンター研究所, 疫学部, 部長 (30150212)
Co-Investigator(Kenkyū-buntansha)	MUNOZ Ivan タラパカ大学, 考古学, 教授 NUNES Lautar 北カトリック大学, 考古学, 教授 CARTIER Luis チリ大学, 医学部・神経内科・病理学, 教授宝来聰国立遺伝学研究所, 人類遺伝学, 助教授 (40126157) 渡辺英伸新潟大学, 医学部・病理学, 教授 (70037381) 園田俊郎鹿児島大学, 医学部・ウイルス学, 教授 (40036463)
Keywords	日本人 / 南米先住民族 / ミイラ / DNA / HTLV-I / 民族疫学 / 免疫遺伝学 / 考古人類学
Research Abstract	アンデス先住民族(アタカマ族)には南西日本に多いヒト白血球ウイルス(HTLV-I)感染者が高頻度(10%)に出現し、しかも彼らは南西日本人と類似したHLA遺伝子を有しており、両者の遺伝学的背景やがん罹患分布には類似性が見られ、民族疫学的にも興味が持たれている。また、チリ北部の砂漠地帯には現在でも数千検体のミイラが比較的好条件で残存している。そこで、今年度はこれら先住民族のミイラを保存しているサンミグエル博物館(アリカ市)やサンペドロ博物館、およびミイラが未だに埋葬されている同地域の古代墓地を訪問し、同博物館の考古学者らの協力を得てミイラ138体(アリカ市96体、サンペドロ市42体)から組織(骨、および骨髄)を採取することがきでた。それらの組織はDNA(核、ミトコンドリア)を検索するために日本まで持ち帰った。まず、サンペドロ博物館の約千年前の標本14検体につき、乾燥量100-500mgを用い、PCR法にてヒト白血球抗原と血色素のDNA断片の抽出を試みた。その結果、4検体からHLADQB1(400bp)とβクロビンDNA(800bp)が得られた。これらの結果を踏まえて、さらに他の検体からのDNA断片の抽出作業を続け、それら抽出したDNA断片の配列を明らかにしていきたい。また、チリ先住民族や日本人に多く検出されているHTLV-IのプロウイルスDNAの抽出も試みたが、現時点ではその抽出には成功してない。ミトコンドリアDNAの抽出にも成功し、その配列について検討中である。次年度も今年度に引き続き、ペル-南部やチリ、ボリビア北部のミイラから検体採取を続け、それらの核DNAやミトコンドリアDNAの抽出を続けていく。それらのDNA情報と現存しているチリ先住民族や日本人のDNAとの比較研究により、民族疫学的、免疫遺伝学的、人類学的、考古学的に有用となるDNA情報として構築していきたい。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] Horai,S.: "Evolution and origins of man:Clues from complete sequences of hominoid mitochondrial DNA" Southeast Asian J.Trop.Med.Public Health. 1(Sup.). 146-154 (1995)
[Publications] La Grranade,L.: "HLA DRB1^<>DQB1^<>haplotype in HTLV-I-associated familial infective dermatitis may predict development of HAM/TSP" Amer.J.Med.Genetics. 61. 37-41 (1995)
[Publications] Takazaki,T.: "Incidence of human T lymphotropic virus type I seroconversion after age 40 among Japanese residents in an area where the virus is endemic" J.Infectious Disease. 171. 559-565 (1995)
[Publications] Tajima,K.: "Epidemiological features of HTLV-I and adult T cell leukemia" Intervirology. 38. 238-246 (1995)
[Publications] Fujiyoshi,T.: "Ethinic segregation of HTLV-I and HTLV-II carriers among South America native Indians" Int.J.Cancer. 63. 510-515 (1995)
[Publications] Miyashita,H.: "Clonig of HLA-A26 cDNA from Japanese donors possesing“ATL-associated HLA haplotypes“" Tissue Antigens. 46. 398-400 (1995)
[Publications] Katahira,Y.: "In vitro induction of cytotoxic T lympho-cytes against HTLV-I-infected T-cells from adult T-cell leukemia patients,asymptomatic HTLV-I carriers and sero-negative healthy donors" Jpn.J.Cancer Res.86. 21-27 (1995)
[Publications] Yoshinaga,M.: "A maternal risk factor for mother-to-child transmission;Viral antigen-producing capacities in culture of cells in peripheral blood and breast milk" Jpn.J.Cancer Res.86. 649-654 (1995)
[Publications] Blank,M.: "Distribution of HLA and haplotypes of Colombian and Jamaican Black populations" Tissue Antigens. 45. 111-116 (1995)
[Publications] Oohashi,Y.: "p53 immunohistochemistry of gallbladder carcinoma:A comparison of PAb 1801 and Do7 antibodies" Acta Medica et Biologica. 43. 53-57 (1995)
[Publications] Kobayashi,M.: "Correlation of p53 protein expression with apoptotic incidence in colorectal neoplasia" Vitchows Archiv. 427. 27-32 (1995)
[Publications] Horai,S.: "Recent African origin of medern humans revealed by complete sequences of hominoid mitochondrial DNAs" Proc.Natl. Acad. Sci.,USA. 92. 532-536 (1995)
[Publications] Hammer,M.: "Y chromosomal DNA variation and the peopling of Japan" Am.J.Hum.Genet.56. 951-962 (1995)
[Publications] Hayashi,S.: "Molecular phylogeny of gibbons inferred from mitochondrial DNA sequences:Preliminary report" J.Mol.Evol.41. 359-365 (1995)
[Publications] Horai,S.: "The Origin and Past of Modern Human as Viewed from DNA(eds.,S.Brenner,K.Hanihara)" World Scientific, 15 (1995)
[Publications] Horai,S.: "Prehistoric Dispersal of Mongoloids(eds.,E.Szathmary,T.Akazawa)" Oxford University Press, 14 (1995)

1995 Fiscal Year Annual Research Report

南米先住民族の人類遺伝学的研究

Principal Investigator

田島 和雄 愛知県がんセンター研究所, 疫学部, 部長 (30150212)

Research Products

[Publications] Horai,S.: "Evolution and origins of man:Clues from complete sequences of hominoid mitochondrial DNA" Southeast Asian J.Trop.Med.Public Health. 1(Sup.). 146-154 (1995)

[Publications] La Grranade,L.: "HLA DRB1^<>DQB1^<>haplotype in HTLV-I-associated familial infective dermatitis may predict development of HAM/TSP" Amer.J.Med.Genetics. 61. 37-41 (1995)

[Publications] Takazaki,T.: "Incidence of human T lymphotropic virus type I seroconversion after age 40 among Japanese residents in an area where the virus is endemic" J.Infectious Disease. 171. 559-565 (1995)

[Publications] Tajima,K.: "Epidemiological features of HTLV-I and adult T cell leukemia" Intervirology. 38. 238-246 (1995)

[Publications] Fujiyoshi,T.: "Ethinic segregation of HTLV-I and HTLV-II carriers among South America native Indians" Int.J.Cancer. 63. 510-515 (1995)

[Publications] Miyashita,H.: "Clonig of HLA-A26 cDNA from Japanese donors possesing“ATL-associated HLA haplotypes“" Tissue Antigens. 46. 398-400 (1995)

[Publications] Katahira,Y.: "In vitro induction of cytotoxic T lympho-cytes against HTLV-I-infected T-cells from adult T-cell leukemia patients,asymptomatic HTLV-I carriers and sero-negative healthy donors" Jpn.J.Cancer Res.86. 21-27 (1995)

[Publications] Yoshinaga,M.: "A maternal risk factor for mother-to-child transmission;Viral antigen-producing capacities in culture of cells in peripheral blood and breast milk" Jpn.J.Cancer Res.86. 649-654 (1995)

[Publications] Blank,M.: "Distribution of HLA and haplotypes of Colombian and Jamaican Black populations" Tissue Antigens. 45. 111-116 (1995)

[Publications] Oohashi,Y.: "p53 immunohistochemistry of gallbladder carcinoma:A comparison of PAb 1801 and Do7 antibodies" Acta Medica et Biologica. 43. 53-57 (1995)

[Publications] Kobayashi,M.: "Correlation of p53 protein expression with apoptotic incidence in colorectal neoplasia" Vitchows Archiv. 427. 27-32 (1995)

[Publications] Horai,S.: "Recent African origin of medern humans revealed by complete sequences of hominoid mitochondrial DNAs" Proc.Natl. Acad. Sci.,USA. 92. 532-536 (1995)

[Publications] Hammer,M.: "Y chromosomal DNA variation and the peopling of Japan" Am.J.Hum.Genet.56. 951-962 (1995)

[Publications] Hayashi,S.: "Molecular phylogeny of gibbons inferred from mitochondrial DNA sequences:Preliminary report" J.Mol.Evol.41. 359-365 (1995)

[Publications] Horai,S.: "The Origin and Past of Modern Human as Viewed from DNA(eds.,S.Brenner,K.Hanihara)" World Scientific, 15 (1995)

[Publications] Horai,S.: "Prehistoric Dispersal of Mongoloids(eds.,E.Szathmary,T.Akazawa)" Oxford University Press, 14 (1995)

田島和雄愛知県がんセンター研究所, 疫学部, 部長 (30150212)