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1996 Fiscal Year Final Research Report Summary

Nucleation and Growth Kinetics in Protein Crystallization

Research Project

Project/Area Number 07044057
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

KOMATSU Hiroshi  Institute for Materials Research, Tohoku University, Professor, 金属材料研究所, 教授 (00108565)

Co-Investigator(Kenkyū-buntansha) THOMAS Bill  Center for Microgravity and Materials Research, University of Alabama, マイクログラビティーセンター, 助手
MUSCHOL Mart  アラバマ大学, マイクログラビティーセンター, 助手
VEKILOV Peter  Center for Microgravity and Materials Research, University of Alabama, マイクログラビティーセンター, 助手
ROSENBERGER Franz  Center for Microgravity and Materials Research, University of Alabama, マイクログラビティーセンター, 教授
SAZAKI Gen  Institute for Materials Research, Tohoku University, 金属材料研究所, 助手 (60261509)
NAKADA Toshitaka  Institute for Materials Research, Tohoku University, 金属材料研究所, 助手 (20237308)
MIYASHITA Satoru  Institute for Materials Research, Tohoku University, 金属材料研究所, 助手 (00219776)
OBINATA Masuo  Institute of development Aging and Cancer, Tohoku Univ., 加齢医学研究所, 教授 (10099971)
MUSHOL Martin  Center for Microgravity and Materials Research, University of Alabama
Project Period (FY) 1995 – 1996
Keywordsproteins / lysozyme / crystal growth / impurity effect / two beam interferometry / light scattering / solubility / effect of magnetic field
Research Abstract

Achievements of this year are as follows ;
1. The Japanese group (J-group) measured solubilities of tetragonal, orthorhombic and triclinic form of hen egg white lysozyme (HEW lysozyme) using two beam interferometry.
2. J-group developed an interferometic method to measure the refractive index inside crystals (HEW lysozyme).
3. J-group got highly purified HEW lysozyme samples using HPLC.With this sample, they observed crystal interfaces using atomic force microscopy. This result was discussed with the American group (A-group) comparing with A-group's result.
4. J-group tried to perform crystallization of HEW lysozyme in a high magnetic field. And they found that it could control the nucleation rate and orientation of crystals.
5. Appoferritin (A-group) and ferritin (J-group) were highly purified and then crystallized. They showed again that removement of impurities is indispensable for growth of crystals of high quality. J-group made a morphodrom of ferritin.
6. A-group performed light scatte … More ring experiments of HEW lysozyme solution and discuss the aggregation process. They estimated the second virial coefficient of the solution and discussed relation between ion intensity and intermolecular interaction.
7. A-group precisely measured topography of vicinal surface with use of the laser interferometry and obtained time evolution of normal and step growth rates and slope of the vicinal surfaces.
8. A-group found that there existed a region where HEW lysozyme solution separated into two liquid phases. They made a phase diagram for this segregation with use of the light scattering technique.
9. In order to estimate the solution layr thickness where convection is ignored, A-grup performed computer simulation of the concentration profiles around a growing crystal changing thickness of the solution layr. J-group measured concentration profiles around a growing lysozyme crystal changing the thickness of the cell. These results were compared each other.
10. J-group measured solubility of HEW lysozyme in situ in high pressure. Pressure dependence of the growth rate was also studied. Less

  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] K.Kurihara,S.Miyashita,G.Sazaki,T.Nakada,Y.Suzuki and H.Komatsu: "Interferometric study on crystal growth of tetragonal lysozyme crystal." Journal of Crystal Growth. 166. 904-908 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] G.Sazaki,K.Kurihara,T.Nakada,S.Miyashita and H.Komatsu: "A novel approach to the solubility measurement of protein crystals by two-beam interferometry." Journal of Crystal Growth. 169. 355-360 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐崎 元、栗原和男、宮下 哲、小松 啓、中田俊隆: "二光束干渉法によるリゾチーム結晶周囲の濃度場観察" 日本結晶成長学会誌. 23. 413-421 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] G.Sazaki,E.Yoshida,H.Komatsu,T.Nakada,S.Miyashita and K.Watanabe: "Effects of a magnetic field on the nucleation and growth of protein crystals" Journal of Crystal Growth. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] E.Kashimoto,G.Sazaki,K.Hasegawa,T.Nakada,S.Miyashita and H.Komatsu: "Study on the crystallization of concanavalin A" Journal of Crystal Growth. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] E.Yoshida,G.Sazaki,T.Nakada,S.Miyashita,H.Komatsu and N.Niimura: "Morphodrom of ferritin crystal" Journal of Crystal Growth. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Sakuma,S.Miyashita,G.Sazaki,T.Nakada and H.Komatsu: "Motion of macro-steps induced on the surface of a lysozyme crystal" Journal of Crystal Growth. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Nakada,G.Sazaki,S.Miyashita and H.Komatsu: "In situ observation of growing surface of lysozyme crystal" Journal of Crystal Growth. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Yanagiya,G.Sazaki,T.Nakada,S.Miyashita and H.Komatsu: "Effect of a magnetic field on the orientation of lysozyme crystal" Journal of Crystal Growth. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Yanagiya,G.Sazaki,T.Nakada,S.Miyashita and H.Komatsu: "Effect of a magnetic field on the nucleation of lysozyme crystal" Journal of Crystal Growth. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] B.R.Thomas,P.G.Vekilov and F.Rosenberger: "Heterogeneity determination and purification of commercial hen egg white lysozyme." Acta Cryst.D52. 776-784 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] P.Vekilov,L.A.Monaco,B.R.Thomas,V.Stojanoff and F.Rosenberger: "Repartitioning of NaCl and protein impurities in lysozyme crystallization." Acta Cryst.D52. 785-798 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] F.Rosenberger: "Protein crystallization" J.Crystal Growth. 166. 40-54 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Muschol and F.Rosenberger: "Lack of evidence for prenucleation aggregate formation in lysozyme crystal growth solutions." J.Crystal Growth. 167. 738-747 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] F.Rosenberger,P.G.Vekilov,M.Muschol and B.R.Thomas: "Nucleation and crystallization of globular proteins-what do we know and what is missing?" J.Crystal Growth. 168. 1-27 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] P.G.Vekilov,J.I.D.Alexander and F.Rosenberger: "Nonliner response of layer growth dynamics in the mixed kinetics-bulk transport regime." Phys.Rev.E.54. 6650-6660 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] P.G.Vekilov,H.Lin and F.Rosenberger: "Unsteady crystal growth due to step-bunch cascading." Phys.Rev.E.55. 3202-3214 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Muschol and F.Rosenberger: "Liquid-liquid phase separation in supersaturated lysozyme solutions : Coupling to precipitate formation and crystallization." Biophys.J.(accepted).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] F.Rosenberger,P.G.Vekilov,H.Lin and J.I.D.Alexander: "A rationale for system-dependent advantages and disadvantates of solution crystal growth at low gravity." Microgravity Sci.Technol.(accepted).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Komatsu,S.Miyashita,T.Nakada,G.Sazaki and A.A.Chernov: ""An atomic level analysis of crystal growth mechanism in complex systems by means of nano-optical microscopy and AFM" in Advances in crystal growth mechanisms" Elsevier,Amsterdam, (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Kurihara, S.Miyashita, G.Sazaki, T.Nakada, Y.Suzuki and H.Komatsu: "Interferometric study on crystal growth o tetragonal lysozyme crystal" Journal of Crystal Growth. 166. 904-908 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] G.Sazaki, K.Kurihara, T.Nakada, S.Miyashita and H.Komatsu: "A novel approach to the solubility measurement of protein crystals by two-beam interferometry." Journal of Crystal Growth. 169. 355-360 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] G.Sazaki, K.Kurihara, S.Miyashu, H.Komatsu and T.Nakada: "In situ Observation of the Concentration Distribution around a Lysozyme Crystal by Two-beam Interferometry" Journal. of the Japanese.Association.for Crystal Growth.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] G.Sazaki, E.Yoshida, H.Komatsu, T.Nakada, S.Miyashita and K.Watanabe: "Effects of a magnetic field on the nucleation and growth of protein crystals" Journal of Crystal Growth. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] E.Kashimoto, G.Sazaki, K.Hasegawa, T.Nakada, S.Miyashita and H.Komatsu: "Study on the crystallization of concanavalin A" Journal of Crystal Growth. (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] E.Yoshida, G.Sazaki, T.Nakada, S.Miyashita, H.Komatsu and N.Niimura: "Morphodrom of ferritin" Journal of Crystal Growth. (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Sakuma, S.Miyashita, G.Sazaki, T.Nakada and H.Komatsu: "Motion o macro-steps induced on the surface of a lysozyme crystal" Journal of Crystal Growth. (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Nakada, G.Sazaki, S.Miyashita and H.Komatsu: "In situ observation of growing surface of lysozyme crystal" Journal of Crystal Growth. (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Yanagiya, G.Sazaki, T.Nakada, S.Miyashita and H.Komatsu: "Effect of a magnetic field on the orientation of lysozyme crystal" Journal of Crystal Growth. (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Yanagiya, G.Sazaki, T.Nakada, S.Miyashita and H.Komatsu: "Effect of a magnetic field on the nucleation of lysozyme crystal" Journal of Crystal Growth. (In preparation).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] B.R.Thomas, P.G.Vekilov and F.Rosenberger: "Heterogeneity determination and purification of commercial hen egg white lysozyme." Acta Cryst.D52. 776-784 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] P.Vekilov, L.A.Monaco, B.R.Thomas, V.Stajanoff and F.Rosenberger: "Repartitioning of NaCl and protein impurities in lysozyme crystallization." Acta Cryst.D52. 785-798 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] F.Rosenberger: "Protein crystallization" J.Crystal Growth. 166. 40-54 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Muschol and F.Rosenberger: "Lack of evidence for prenucleation aggregate formation in lysozyme crystal growth solutions." J.Crysal Growth. 167. 738-747 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] F.Rosenberger, P.G.Vekilov, M.Muschol and B.R.Thomas: "Nucleation and crystallization of globular proteins-what do we know and what is missing?" J.Crystal Growth. 168. 1-27 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] P.G.Vekilov, J.I.D.Alexander and F.Rosenberger: "Nonlinear response of layr growth dynamics in the mixed kinetics-bulk transport regime." Phys.Rev.E.54. 6650-6660 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] P.G.Vekilov, H.Lin and F.Rosenberger: "Unsteady crystal growht due to step-bunch cascadig." Phys.Rev.E.55. 3202-3214 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Muschol and F.Rosenberger: "Liquid-liquid phase separation in supersaturated lysozyme solutions : Coupling to precipitate formation and crystallization." Biophys.J.(accepted).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] F.Rosenberger, P.G.Vekilov, H.Lin and J.I.D Alexander: "A rationale for system-dependent advantages and disadvantates of solution crystal growth at low gravity." Microgravity Sci.Technol.(accepted).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Komatsu, S.Miyashita, T.Nakada, G.Sazaki and A.A.Chernov: Elsevier, Amsterdam. "An atomic level analysis of crystal growth mechanism in complex systems by means of nano-optical microscopy and AFM" in advances in crystal growth mechanisms, (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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