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1996 Fiscal Year Final Research Report Summary

Modulatory mechanisms of cardiac ion channels.

Research Project

Project/Area Number 07044233
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionTokyo Medical and Dental University

Principal Investigator

HIRAOKA Masayasu  Medical Research Institute, Tokyo Medical and Dental University, Professor, 難治疾患研究所, 教授 (80014281)

Co-Investigator(Kenkyū-buntansha) HIRANO Yuji  Medical Research Institute, Tokyo Medical and Dental University, Assistant Profe, 難治疾患研究所, 助手 (00181181)
KAWANO Seiko  Medical Research Institute, Tokyo Medical and Dental University, Associate Profe, 難治疾患研究所, 助教授 (00177718)
HARTZELL Criss  School of Medicine, Emory University, Professor, 医学部, 教授
FOZZARD Harry A  School of Medicine, University of Chicago, Professor, 医学部, 教授
Project Period (FY) 1995 – 1996
Keywordsprotein phosphorylation / L-type Ca^<2+> channel / ATP-sensitive / K^+ channel / HERG K^+ channel / Ca^<2+> -activated Cl^- -channel / Ryanodine receptor channel
Research Abstract

The collaboration with Professor H.A.Fozzard has made a great advance in the technical achievements of the expressed channels functions and structural confirmation. We also acquired the clones of ATP-sensitive K^+ channel. Now, we are ready to express these K^+ channels and functional modulation of HERG K channel by tyrosin phosphorylation is under investigation. Furthermore, mutation of HERG K^+ channels is one of etiological factors for long QT syndrome. Therefore, we started to examine functional abnormality of the mutated channels. The joint research with Professor H.C.Hartzell has made a great advance for the purification of ryanodine receptor channel proteins, which enables us to study their function with much higher success rate. Modulation of Ca^<2+> -activated Cl^- -channel and L-type Ca^<2+> channel by protein phosphorylation and phosphatases is now studied with refined technique and new information owing to the fruits of this joint research.

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Zheng S,Hirano Y,Hiraoka M.: "Arginine-vasopressin induced potentiation of unitary L-type calcium channel current in guineapig ventricular myocytes." Circ.Res.76. 559-595 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawano S,Hirayama Y,Hiraoka M.: "Activation mechanism of [Ca^<2+>]i-sensitive transient outward current in rabbit ventricular myocytes." J.Physiol.486. 593-604 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ono M,Sunami A,Hiraoka M.: "Interaction between external Na^+ and mexiletine on Na^+ channel in guinea-pig ventricular myocytes." Pflugers Arch.431. 101-109 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirano Y,Yoshinaga T,Niidome T,Katayama K,Hiraoka M.: "Modulation by dihydropyridine and protein kinases of the recombinant cardiac L-type Ca channel with multiple unitary current amplitudes." Receptors and Channels. 4. 93-104 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihara K,Hiraoka M,Ochi R.: "The tetravalent orgenic cation spermine causes the gating of the IRK1 channel expressed in murine fibroblast cells." J.Physiol.491. 367-382 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furukawa T,Yamane T,Terai T,Katayama Y,Hiraoka M.: "Functional linkage of the cardiac ATP-sensitive K^+ channels to actin cytoskeleton." Pflugers Arch.431. 504-512 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sunami A,Hiraoka M.: "Blockade of cardiac Na^+ channels by a charged class I antiarrhythmic agent,bisaramil:possible interaction of the drug with a pre-open closed state." Eur.J.Pharmacol. 312. 245-255 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiraoka M,Sawanobori T,Kawano S,Hiraono Y,Furukawa T.: "Functions of cardiac ion channels under normal and pathological conditions." Jpn.Heart J.37. 693-703 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiraoka M.: "Pathophysiological functions of ATP-sensitive K^+ channels in myocardial ischemia." Jpn.Heart J.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiraoka M,Furukawa T.: "Functional Modulation of cardiac ATP-sensitive K^+ channels." News in Physiol.Sci.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiraoka M,Furukawa T,Sawanobori T,Yamane T.: "Regulation of rundown and reactivation of cardiac ATP-sensitive K^+ channels.In ″Molecular and Cellular Mechanisms of Cardio-vascular Regulations.″" Springer-Verlag,Tokyo, 83-91 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zheng S,Hirano Y,Hiraoka M.: "Arginine-vasopressin induced potentiation of unitary L-type calcium channel current in guinea-pig ventricular myocyte." Circ.Res.76. 592-595 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano S,Hirayama Y,Hiraoka M.: "Activation mechanism of [Ca^<2+>] i-sensitive transient outward current in rabbit ventricular myocytes." J.Physiol.486. 593-604 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ono M.Sunami A,Hiraoka M.: "Interaction between external Na^+ and mexiletine on Na^+ channel in guinea-pig ventricular myocytes." Pflugers Arch.431. 101-109 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirano Y,Yoshinaga T,Niidome T,Katayama K,Hiraoka M.: "Modulation by dihydropyridine and protein kinases of the recombinant cardiac L-type Ca channel with multiple unitary current amplitudes." Receptors and Channels. 4. 93-104 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihara K,Hiraoka M,Ochi R.: "The tetravalent organic cation spermine causes the gating of the IRK1 channel expressed in murine fibroblast cells." J.Physiol.491. 367-382 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furukawa T,Yamane T,Terai T,Katayama Y,Hiraoka M.: "Functional linkage of the cardiac ATP-sensitive K^+ channels to actin cytoskeleton." Pflugers Arch.431. 504-512 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sunami A,Hiraoka M.: "Blocade of cardiac Na^+ channels by a charged class I antiarrhythmic agent, bisaramil : possible interaction of the drug with a pre-open closed state." Eur.J.Pharmacol.312. 245-255 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiraoka M,Sawanobori T,Kawano S,Hirano Y,Furukawa T.: "Functions of cardiac ion channels under normal and pathological conditions." Jpn.Heart J.37. 693-704 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiraoka M.: "Pathophysiological functions of ATP-sensitive K^+ channels in myocardial ischemia." Jpn.Heart J.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiraoka M,Furukawa T.: "Functional modulation of cardiac ATP-sensitive K^+ channels." News in Physiol.Sci.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiraoka M,Furukawa T,Sawanobori T,Yamane T.: Springer-Verlag, Tokyo. Regulation of rundown and reactivation of cardiac ATP-sensitive K^+ channels. In "Molecular and Cellular Mechanisms of Cardiovascular Regulations"., 83-91 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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