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1995 Fiscal Year Final Research Report Summary

Functional molecules on glomerular epithelial cells

Research Project

Project/Area Number 07044235
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionNiigata University

Principal Investigator

SHIMIZU Fujio  Institure of Nephrology, Niigata University School of Medicine professor, 医学部, 教授 (40012728)

Co-Investigator(Kenkyū-buntansha) KAWACHI Hiroshi  Institute of Nephrology, Niigata University School of Medicine Research Assistan, 医学部, 助手 (60242400)
MORIOKA Tetsuo  Institute of Nephrology, Niigata University School of Medicine Research Assistan, 医学部, 助手 (00210146)
SALANT David j.  Rend section, Boston University Medical Center professor, 医学部, 教授
Project Period (FY) 1995
KeywordsMonoclona antibody / Proteinuria / Slit diaphragm / Podocyte / Glomerular permeability / Functional molecule / Cultured glomerular epithelial cell
Research Abstract

Cultured glomerular epithelial cells in the loboratory of Dr. Salant in Boston University could not be stained by the monoclonal antibody (mAb) 5-1-6.
Cloning of the target antigenic molecule has not been succeeded, using the rat kidney cDNA library prepared in the Boston laboratory.
The traial to get the antigenic molecule has been repeated in vain, using the affinity column with in vitro prepared- and purified-mAb 5-1-6 and the solublized rat glomeruli.
The biogenesis of the target antigen of mAb 5-1-6 was studied in the developing glomerulus by immunolocalization and metabolic labeling. This antigen first became faintly, but clearly, detectable on the basal and lateral sides of the developing podocytes at the S-shaped body stage. Staining intensity increased with further maturation and was restricted to the visceral epithelial cells. On immunoelectro microscopy, the antigen was seen along the basal and lateral surfaces below occluding junctions at the early capillary loop stage and later, with the interdigitation of foot processes, became concentrated in the slit pores. At no stage was the antigen seen on the apical surface. Metaboric labeling studies showed that the antigenic molecule is actively synthesized during initial glomerular development and that the rate of synthesis declines substantially with maturation.
From these results cDNA-library was considered to be more poperly prepared from rat glomeruli in neonatal stage for cloning of this antigenic molecule. We are planning to clone this, applying the cDNA library from baby glomeruli.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] D.Gollner,H.Kawachi,T.Oite,M.Oka,M.Nagase,F.Shimizu: "Strain variation in susceptibility to the development of monoclonal antibody 5-1-6 induced proteinuria in rats" Clinical and Experimental Immunology. 101. 341-345 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] E.Yaoita,T.Yamamoto,N.Takashima K.Kawasaki,H,Kawachi,F.Shimizu I.Kihara: "Visceral epithelial cells in rat glomerular cell culture" European Journal of Cell Biology. 67. 131-144 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kawachi,D.R.Abrahamson P.L.St.John,D.J.Goldstein,M.A.Shia,K.Matsui,F.Shimizu: "Developmental expression of the nephritogenic antigen of monoclonal antibody 5-1-6" American Journal of Pathology. 147. 823-833 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水不二雄(荒川正昭・長澤俊彦 編): "最新内科学大系 腎・泌尿器 1 ネフロン障害・糸球体疾患の発症機序" 中山書店(東京), 325(277-293) (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] D.J.Salant,Y.Natori,F.Shimizu (cd.by E.G.Neilson,W.G.Couser): "Immunologic Renal Diseases Glomerular injury due to antibody alone" Raven Press (New York)(印刷中), (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] D.Gollner, H, Kawachi, T.Oka, M.Nagase, F.Shimizu: "Strain variation in susceptibility to the development of monoclonal antibody 5-1-6 induced proteinuria in rats" Clinical and Experimental Immunology. 101. 341-345 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] E.Yaoita, T.Yamamoto, N, Takashima, K.Kawasaki, H.Kawachi, F.Shimizu, I.Kihara: "Visceral epithelial cells in rat glomerular cell culture" European Journal of Cell Biology. 67. 131-144 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Kawachi, D.R.Abrahamson, P.L.St.John, D.J.Goldstein, M.A.shia, K.Matsui, F.Shimizu: "Developmental expression of the nephritogenic antigen of monoclonal antibody 5-1-6" American Journal of Pathology. 147. 823-833 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] F.Shimizu: Nakayama (Tokyo). Pathogenesis of glomerular diseases. in Diseasis of Nephron (ed by M.Arakawa, T.Nagasawa), 277-293 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] D.J.Salant, Y.Natori, F.Shimizu: Raven Press (New York) (in press). Glomerular injury due to antibody alone. in Immunologic Renal Diseasis (ed by E.G.Neilson, W.G.Couser), (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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