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1997 Fiscal Year Final Research Report Summary

Japan-French Joint Research on Genome

Research Project

Project/Area Number 07044266
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Molecular biology
Research InstitutionInternational Institute for Advanced Studies (1997)
Osaka University (1995-1996)

Principal Investigator

MATSUBARA Kenichi  International Institute for Advanced Studies, Vice Director, 副所長 (20037394)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Hiroshi  Nara Institute of Science and Technology, Graduate School of Biological Sciences, バイオサイエンス研究科, 教授 (70019876)
FUJIYAMA Asao  National Institute of Genetics, Division of Human Genetics, Associate Professor, 総合遺伝研究系, 助教授 (60142311)
SAKAKI Yoshiyuki  The University of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (10112327)
OGASAWARA Naotake  Nara Institute of Science and Technology, Graduate School of Biological Science,, バイオサイエンス研究科, 教授 (10110553)
ITOH Takashi  The University of Tokyo, Institute of Medical Science, Assistant Professor, 医科学研究所, 助手 (90201326)
Project Period (FY) 1995 – 1997
KeywordsGenome research / Structural analyzes / Functional analysis / Genome informatics / Human genome / Analysis of B.subtilis / Yeast genome / Database
Research Abstract

To meet the need for deeper collaborative efforts on human disease genes, genome structure of model organisms, genome informatics, two French scientists, Drs.P.Bouvagnet and J.Thierry-Mieg were added to the member's list. Both of them came to Japan for collaborative researches.
Based upon agreement between Matsubara and Dr.F.Galibert at CNRS,special efforts have been made to promote collaborative activities that include such researchers as Sakaki, Ohki, Fujiyama (human genome structure), Yoshikawa, Ogasawara, Murakami, Ito (microbial genome structure), Takagi (genome informatics), Matsubara (cDNA). Matsuda (Research collaborator) visited Pasteur Institute and CNRS Montpellier to carry out collaboration on human immunoglobulin gene. Exchanges of young researchers were also actively promoted. French scientists who came to Japan for collaboration included P.Bouvagnet, P.Slonimski, A.Danchin, J.Thierry-Mieg.
On December 1997, Matsubara, Fujiyama, along with Wakasugi (Research collaborator) visited Paris and dislcussed with Drs.Samarut (CNRS Life Science Director) and Galibert (do., Vice director), summarizing achievements in these three years and exchanged ideas on future perpectives. As a result, the following consensus was obtrained. (1) This collaboration program has greatly helped mutual understanding, generating reliable and deep interactions among the participants, and as a result several new collaborative efforts have been initiated. (2) Ongoing collaborative researchers should be enforced. (3) Supports for exchanges should be sought for futhersing mutual understanding and stimulations ; especially encouraging exchange of young researchers for better interactions for the future.
The resume of the three years' activities will be published in an well-circulated international journal.

  • Research Products

    (53 results)

All Other

All Publications (53 results)

  • [Publications] Sumimoto, H.(Itoh, T): "Membrane translocation of cytosolic factors in activation of the phagocyte NADPH oxidase : role of protein-protein interactions" Membrance proteins:Structure,Function and Expression Control(eds.Mihara,K.& Hamasaki,N.)S.Karger AG,Switzerland. 235-245 (1997)

    • Description
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  • [Publications] Kito, K.(Ito, T.Sakaki, Y.): "Fluorescent differential display analysis of gene expression in differentiating neuroblastoma cells" Gene. 184. 73-81 (1997)

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  • [Publications] Ito, T. and Sasaki, Y.: "Fluorescent differential display : a fast and safe way for reliable differential display analysis" Fingerprinting Methods Based on Arbitrarily Primed PCR(eds.Micheli,M.R. & Bova,R.)Springer-Verlag,Heidelberg. 305-313 (1997)

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  • [Publications] Furihata, C.(Itoh, T.): "Differentially expressed MHC class II-associated invariant chain in rat stomach pyloric mucosa with N-methyl-N^1nitro-N-nitrosoguanidine exposure" Cancer Res.57. 1416-1418 (1997)

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  • [Publications] Furihata, C.(Itoh, T.): "Rare occurrence of ras and p53 gene mutation in mouse stomach tumors induced N-methyl-N-nitrosourea" Jpn.J.Cancer Res.88. 363-368 (1997)

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  • [Publications] Ito, T.and Sakaki, Y.: "Fluorescent differential display" Methods Mol.Biol.,Differential Display Methods and Protocoles(eds.Liang,P.& Pardee,A.B.)Humana Press Inc.,Totowa,NJ. 85. 37-44 (1997)

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  • [Publications] Adaci, N.(Itoh, T.): "Isolation and expression study of a maternally expressed novel Xenopus gene Xeml encoding a putative evolutionarily conserved membrane protein" Biochem.Biophys.Res.Commun.238. 899-904 (1997)

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  • [Publications] Hagiwara, Y.(Itoh, T.): "Screening for imprinted genes by allelic messege display:Identification of a paternally expressed gene Impact on mouse chromosome 18" Proc.Natl.Acad.Sci.USA. 94. 9249-9254 (1997)

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  • [Publications] Mizuki, K.(Itoh, T.): "Functional modules and expression of mouse p40^<phox> and 67^<phox>,Src homology 3(SH3)domain-containing proteins involved in the phagocyte NADPH oxidase complex" Eur.J.Biochem.251. 573-582 (1998)

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  • [Publications] Nakamura, R.(Itoh, T.): "The PC motif: A novel and evolutionarily conserved sequence involved in interaction between p40^<phox> and 67^<phox>,SH3 domain-containing cytolsolic factors of the phagocyte NADPH oxidase" Eur.J.Biochem.251. 583-589 (1998)

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  • [Publications] Hata, K.(Itoh, T.): "Anionic amphiphile-independent activation of the phagocyte NADPH oxidase in a cell-free system by p47^<phox> and 67^<phox>,both in C terminally truncated forms:Implication for regulatory SH3 domain-mediated interactions" J.Biol.Chem.273. 4232-4236 (1998)

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  • [Publications] Ito, T.and Sakaki, Y.: "Fluorescent differential display(FDD):A fast and reliable method for messege display PCR" Methods Enzymol,inpress.

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  • [Publications] Arai, Y.(Ohki, M.): "The inv(11)(p15q22)chromosome translocation of de nove and therapy-related myeloid malignancies results in fusion of the nucleoporin gene,NUP98,with the putative RNA helicase gene,DDX10" Blood. 89. 3936-3944 (1997)

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  • [Publications] Hosoda, F.(Ohki, M.): "A complete Not1 restiction map covering the entire long arm of human chromosome 11" Genes to Cells. 2. 345-347 (1997)

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  • [Publications] Kitamura, E.(Ohki, M.): "A3-Mb sequence-ready contig map encompassing the multiple disease gene cluster on chromosome 11q13.1-q13.3" DNA Res.4. 281-289 (1997)

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  • [Publications] Kunst, F.(Ogasawara, N.): "The complete genome sequence of the gram-positive bacterium Bacillus subtilis" Nature. 390. 249-256 (1997)

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  • [Publications] Kasahara, Y.(Ogasawara, N.): "Sequence analysis of the groESL-cotA region of the Bacillus subtilis genome,containing the restriction/modification system gene" DNA Res.4. 335-339 (1997)

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  • [Publications] Liu, H.(Ogasawara, N., Yoshikawa, H.): "Sequence analysis of a 25-kb segment in the 17-19 degree region of the Bacillus subtilis chromosome containing ada locus" DNA Res.4. 325-328 (1997)

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  • [Publications] Beloin, C.(Ogasawara, N.): "Characterization of a lrp-like gene Bacillus subtilis" Mol.Gen.Genet. 256. 63-71 (1997)

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  • [Publications] Sadaie, Y.(Ogasawara, N.): "Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome" Microbiology. 143. 1861-1866 (1997)

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  • [Publications] Kasahara, Y.(Ogasawara, N.): "Sequence analysis of the 36kb region between gnte and trny genes of Bacillus subtilis genome" DNA Res.4. 155-159 (1997)

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  • [Publications] Coillie, E.V.(Sakaki, Y.): "The human MCP-2 gene(SCYA8):cloning,sequence analysis,tissue expression,and assignment to the CC chemokine gene contig on chromsome 17q11.2" Genomics. 40. 323-331 (1997)

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  • [Publications] Kamino, K.(Sakaki, Y.): "A variety of presenilin 1 mutations in Japanese familial early-onset Alzheimer's disease" Alzheimer's Disease : Biology,Diagnosis and Terapeutics. 79-84 (1997)

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  • [Publications] Hattori, M.(Sakaki, Y.): " A novel method for making nested deletions and its application for sequencing of a 300kb region of human APP locus" Nucleic Acids Research. 25. 1802-1808 (1997)

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  • [Publications] Hata, K.(Sakaki, Y.): "Identification of critical CpG sites for repression of L1 transcription by DNA methylation" Gene. 189. 227-234 (1997)

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  • [Publications] Ohira, M.(Sakaki, Y.): "Gene identification in 1.6-Mb region of the Down syndrome region on chromosome 21" Genome Res.47. 47-58 (1997)

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  • [Publications] Nakamura, A.(Sakaki, Y.): "Isolation of a novel human gene from the down syndrome critical region on chromosome 22q22.2" J.Biochem.122. 872-877 (1997)

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  • [Publications] Nakamura, A.(Sakaki, Y.): "A novel gene isolation from human placenta located in Down syndrome critical region on chromosome 21" DNA Res.4. 321-324 (1997)

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  • [Publications] Tei, H.(Sakaki, Y.): "Circadian oscillation of a mammalian homologue of the Drosophila period gene" Nature. 389. 512-516 (1997)

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  • [Publications] Tsukahara, F.(Sakaki, Y.): "Molecular characterization of mouse mtprd gene,the homologue oh human TPRD:the unique gene expression suggesting its critical role in pathophysiology of Down syndrome" J.Biochem.,in press.

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  • [Publications] Tsukamoto, Y.(Takagi, T.): "Application of a deductive database system to search to search for topological and similar three-demensional structures in protein" Computer Applications in the Biosciences. 13. 183-190 (1997)

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  • [Publications] Ohta, Y.(Takagi, T.): "Automatic construction of knowledge base from biological papers" Proc.of the Fifth International Conference on Intelligent Systems for Molecular Biology. 218-225 (1997)

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  • [Publications] Satou, K.(Takagi, T.): "Extraction of substructures of proteins essential to their biological functions by a data mining technique" Proc.of the Fifth International Conference on Intelligent Systems for Molecular Biology. 254-257 (1997)

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  • [Publications] Tsunoda, T.(Takagi, T.): "Automatic extraction of position specific cooccurence of transcription factor bindings on promoters" Proc.of the Pacific Symposium on Biocomputing '98. 252-263 (1998)

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  • [Publications] Fukuda, K.(Takagi, T.): "Toward information extraction: Identifying protein names from biological papers" Proc.of the Pacific Symposium on Biocomputing '98. 707-718 (1998)

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  • [Publications] Tenzen, T.(Fujiyama, A.): "Precise switching of DNA replication timing in the GC content transition area in the human major histocompatibility complex" Mol.Cell.Biol.17. 4043-4050 (1997)

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  • [Publications] 藤山 秋佐夫: "二次元電気泳動によるゲノムスキャンニング法" シリーズ・ニューバイオフィジックス「ヒューマンゲノム計画」(金久 實編). (1997)

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  • [Publications] 藤山 秋佐夫: "ゲノムリソースの作成とゲノム生物学への適用" 蛋白質核酸酵素. 42. 2472-2747 (1997)

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  • [Publications] Okubo, K.and Matsubara, K.: "Complementary DNA sequence(EST)collections and the expression information of the human genome" FEBS Letters. 403. 225-229 (1997)

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  • [Publications] Maeda, K.(Matsubara, K.): "Analysis of an expression profile of genes in the human adipose tissue" Gene. 190. 227-235 (1997)

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  • [Publications] Yoshikawa, H.(Fujiyama, A., Matsubara, K.): "Chromosome assignment of aberrant NotI restriction DNA fragments in primary hepatocellular carcinoma" Gene. 197. 129-135 (1997)

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  • [Publications] Matsumoto, A.(Matsubara, K.): "An expression profile of genes in human retina and isolation of a cDNA for a novel rod photoreceptor protein" IOVS. 38. 2576-2585 (1997)

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  • [Publications] Matsuo, N.(Matsubara, K.): "Cloning of a cDNA encoding a novel sugar transporter expressed in the neonatal mouse hippocampus" Biochem.Biophys.Res.Commun.238. 126-129 (1997)

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  • [Publications] Kuriyama, H.(Matsubara, K.): "Molecular cloning and expression of a novel aquaporin from adipose tissue with glycerol permeability" Biochem.Biophys.Res.Commun.241. 53-58 (1997)

    • Description
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  • [Publications] Matsumoto, A.(Matsubara, K.): "An expression profile of genes in human retina and isolation of a cDNA for a novel rod photoreceptor protein" IOVS. 38. 2576-2585 (1997)

    • Description
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  • [Publications] Matsuo, N.(Matsubara, K.): "Cloning of a cDNA encoding a novel sugar trensporter expressed in the neonatal mouse hippocampus" Biochem.Biophys.Res.Commun.238. 126-129 (1997)

    • Description
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  • [Publications] Kuriyama, H.(Matsubara, K.): "Molecular cloning and expression of a novel aquaporin from adipose tissue with glycerol permeability" Biochem.Biophys.Res.Commun.241. 53-58 (1997)

    • Description
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  • [Publications] Eki, T.(Murakami, Y.): "Cloning and characterization of novel gene DCRR1,expressed from Down's syndrome critical region of human chromosome 21q22.2" DNA Sequence. 7. 153-164 (1997)

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  • [Publications] Ohiara, M.(Murakami, Y.): "Gene idendification in 1.6-Mb region of the Down syndrome region on chromosome 21" Genome Res.7. 47-58 (1997)

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  • [Publications] Naitou, M.(Murakami, Y.): "Expression profiles of transcripts from 126 ORFs in Saccharomyces cerevisiae chromosome VI by systematic Northern analyse" Yeast. 13. 1275-1290 (1997)

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  • [Publications] Ishiai, M.(Murakami, Y.): "Isolation of human and fission yeast gene homologues of the budding yeast origin recognition complex subunit Orc5:Human homologue(ORC5L)maps to chromosome band 7q22" Genomics. 46. 294-298 (1997)

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  • [Publications] Sakamoto, A.(Murakami, Y.): "Both hypertronic and dilated cardiomyopatgies are caused by mutation of the same gene delta-sarcoglycan,in hamster:An animal model of disrupted dystrophin-associated glycoprotein complex" Proc.Natl.Acad.Sci.USA. 94. 13873-13878 (1997)

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  • [Publications] Eki, T.(Murakami, Y.): "Mapping of the human genes encoding cyclin H(CCHH)and the CDK-activating kinase(CAK)assembly factor MAT1(MNAT1)to chromosome bands 5q13.3-q14 and 14q23,respectively" Genomics. (in press).

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Published: 1999-03-16  

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