1995 Fiscal Year Annual Research Report

バゾプレッシン・オキシトシンによる生体調節機序の解明-分子生物学・形態学・薬理学及び生理学的アプローチ-

Research Project

Project/Area Number	07044296
Research Institution	University of Occupational and Environmental Health, Japan
Principal Investigator	山下博産業医科大学, 医学部, 教授 (00030841)
Co-Investigator(Kenkyū-buntansha)	澁谷泉産業医科大学, 医学部, 助教授 (50162649) 稲永清敏九州歯科大学, 歯学部, 教授 (90131903) 河田光博京都府立医科大学, 医学部, 教授 (60112512) DREIFUSS J.J ジュネーブ大学, 医学部, 教授 KOIZUMI K ニューヨーク州立大学, 医学部, 教授 PFAFF D.W ロックフェラー大学, 神経生物&行動, 教授
Keywords	バゾプレッシン / オキシトシン / 遺伝性多飲マウス / 遺伝子解析 / In situ hybridization法 / パッチクランプ法 / カルシウム画像解析
Research Abstract	バゾプレッシンおよびオキシトシンの生体への生理作用をラットおよびマウス(特に遺伝性多飲マウスを主に)を用いて多方面から解析中である。今年度に得られた成果を以下に列記する。1.ラットにバゾプレッシンのアンチセンスDNAを脳内投与し、飲水量および尿量を観察した。バゾプレッシンのアンチセンスDNAの脳内投与により多飲、多尿が引き起こされた。遺伝性多飲マウスのバゾプレッシン遺伝子の塩基配列を決定した。遺伝性多飲マウスのバゾプレッシン遺伝子の塩基配列は、コントロールマウスとは1塩基のみ異なっていたが、アミノ酸のコードには影響のない部位であった。現在、バゾプレッシン受容体遺伝子の塩基配列を解析中である。2.バゾプレッシンおよびオキシトシンの初期核内遺伝子(hnRNA)をIn situ hybridization法により可視化するために、イントロンを含むcDNAを調整した。遺伝性多飲マウスのバゾプレッシンおよびオキシトシンmRNAの脳内の局在を明らかにした。遺伝性多飲マウスのバゾプレッシンおよびオキシトシンmRNAの脳内の局在は、バゾプレッシンおよびオキシトシンの抗体を用いた免疫組織染色法による細胞体の分布と一致した。バゾプレッシン遺伝子は視床下部で過剰に発現していた。3.パッチクランプ法を用いて、脳スライス標本および単離した神経分泌細胞からの電気記録を行った。カルシウム画像解析装置を用いて、脳スライス標本および単離した神経細胞から細胞内カルシウム濃度の測定法を確立した。パッチクランプ法を用いても、脳スライス標本および単離した神経分泌細胞からバゾプレッシンおよびオキシトシンニューロンに特徴的な電気活動を記録できた。カルシウム画像解析装置を用いて、脳スライス標本および単離した神経細胞のグルタミン酸による細胞内カルシウム濃度の上昇を可視化できた。

Research Products

(19 results)

All Other

All Publications (19 results)

[Publications] Ueta,Y.et al.: "Water deprivation induces regional expression of c-fos protein in the brain of inbred polydipsic mice." Brain Research. 677. 221-228 (1995)
[Publications] Nagatomo,T.et al.: "Transient outward current in adult rat supraoptic neurones with slice patch-clamp technique : inhibition by angiotensin II." Journal of Physiology. 485. 87-96 (1995)
[Publications] Yamaguchi,K.et al.: "Possible existence in the brain of neural mechanisms involved in inhibition of vasopressin release in the presence of an osmotic stimulus and angiotensin II." Exp.Clin.Endocrinol.14. 13-16 (1995)
[Publications] Ueta,Y.et al.: "Does recurrent inhibition exist in the paraventricular magnocellular neurosecretory cells in the male rat?" Japanease Journal of Physiology. 45. 913-919 (1995)
[Publications] Tanaka,K.et al.: "Potensiation of cholesystokinin-induced amylase release by peptide VIP in guinea pig pancreas acini." Japanease Journal of Physiology. 45. 241-256 (1995)
[Publications] Ueta,Y.et al.: "Adrenomedullin-immunoreactive neurons in the paraventricular and supraoptic nuclei of the rat." Neuroscience Letters. 202. 1-4 (1995)
[Publications] Yamashita,H.: "Fourth International Workshop of UOEH on Emotion,Stress and Metabolism" Journal of UOEH. 17. 299-320 (1995)
[Publications] Osaka,T.et al.: "Activity of neurons in the lateral preoptic area during drinking behavior by the rat." Brain Research Bulletin. 36. 361-369 (1995)
[Publications] Osaka,T.et al.: "Osmosensitive hypothalamic neurons and their responses to cardiovascular receptor activation." Brain Research Bulletin. 36. 371-379 (1995)
[Publications] Yamamoto,S.et al.: "Cardiovascular-related peptides influence hypothalamic neurons involved in control of body water homeostasis" Obesity Research. 3. 789 (1995)
[Publications] Inenaga,K.et al.: "GABA ergic inhibitory inputs to subfornical organneurons in rat slice preparations" Brain Research. 705. 85-90 (1995)
[Publications] Tanaka,H.et al.: "Biphasic changes in behavioral,endocrine,and sympathetic systems in adjuvant arthoitis in Lewis rats" Brain Research Bulletin. 39. 33-37 (1996)
[Publications] Niwa,K.et al.: "Temperature dependence of processes proxinal and distal to Glucose-induceel [Ca^<2+>] i rise in stimulus-secretion coupling in rat pancreatic islets" Biosignalling. (in press).
[Publications] Tanaka,K.et al.: "PACAP causes rapid Ca^<2+> release from intracellilar stores and long lasting Ca^<2+> influx mediated by Na^+ influx-dependent membrane depolarization in bouine adrenal chromsffin cells" Endocrinology. (in press).
[Publications] Funabashi,T.et al.: "Changes in preproenkephalin messenger RNA leuel in the rat ventromedial hypothalamus during the estrous cycle" Molecular Brain Research. 28. 129-134 (1995)
[Publications] Kumamoto,K.et al.: "Oxytocin-producing and vasopressin-producing eosinophils in the mouse spleen : immunohistochemical immuno-electron-microscopic and in situ hykrization studies" Cell Tissue Research. 281. 1-10 (1995)
[Publications] Ueda,S.et al.: "Role of neuropeptide Y projection on the development of serotonergic innervation in the suprachiasmatic nucleus of the rat,shown by tliple intraocular grafts" Brain Research. 673. 325-330 (1995)
[Publications] Yamashita,H.et al.: "Neurohypophysis : Recent Progress of Vasopressin and Oxytocin Research" Elsevier Science B.V., 197-204 (1995)
[Publications] 大坂寿雅 et al.: "THERAPEUTIC RESEARCH" ライフサイエンス出版株式会社, 32-35 (1995)

1995 Fiscal Year Annual Research Report

バゾプレッシン・オキシトシンによる生体調節機序の解明-分子生物学・形態学・薬理学及び生理学的アプローチ-

Principal Investigator

山下 博 産業医科大学, 医学部, 教授 (00030841)

Research Products

[Publications] Ueta,Y.et al.: "Water deprivation induces regional expression of c-fos protein in the brain of inbred polydipsic mice." Brain Research. 677. 221-228 (1995)

[Publications] Nagatomo,T.et al.: "Transient outward current in adult rat supraoptic neurones with slice patch-clamp technique : inhibition by angiotensin II." Journal of Physiology. 485. 87-96 (1995)

[Publications] Yamaguchi,K.et al.: "Possible existence in the brain of neural mechanisms involved in inhibition of vasopressin release in the presence of an osmotic stimulus and angiotensin II." Exp.Clin.Endocrinol.14. 13-16 (1995)

[Publications] Ueta,Y.et al.: "Does recurrent inhibition exist in the paraventricular magnocellular neurosecretory cells in the male rat?" Japanease Journal of Physiology. 45. 913-919 (1995)

[Publications] Tanaka,K.et al.: "Potensiation of cholesystokinin-induced amylase release by peptide VIP in guinea pig pancreas acini." Japanease Journal of Physiology. 45. 241-256 (1995)

[Publications] Ueta,Y.et al.: "Adrenomedullin-immunoreactive neurons in the paraventricular and supraoptic nuclei of the rat." Neuroscience Letters. 202. 1-4 (1995)

[Publications] Yamashita,H.: "Fourth International Workshop of UOEH on Emotion,Stress and Metabolism" Journal of UOEH. 17. 299-320 (1995)

[Publications] Osaka,T.et al.: "Activity of neurons in the lateral preoptic area during drinking behavior by the rat." Brain Research Bulletin. 36. 361-369 (1995)

[Publications] Osaka,T.et al.: "Osmosensitive hypothalamic neurons and their responses to cardiovascular receptor activation." Brain Research Bulletin. 36. 371-379 (1995)

[Publications] Yamamoto,S.et al.: "Cardiovascular-related peptides influence hypothalamic neurons involved in control of body water homeostasis" Obesity Research. 3. 789 (1995)

[Publications] Inenaga,K.et al.: "GABA ergic inhibitory inputs to subfornical organneurons in rat slice preparations" Brain Research. 705. 85-90 (1995)

[Publications] Tanaka,H.et al.: "Biphasic changes in behavioral,endocrine,and sympathetic systems in adjuvant arthoitis in Lewis rats" Brain Research Bulletin. 39. 33-37 (1996)

[Publications] Niwa,K.et al.: "Temperature dependence of processes proxinal and distal to Glucose-induceel [Ca^<2+>] i rise in stimulus-secretion coupling in rat pancreatic islets" Biosignalling. (in press).

[Publications] Tanaka,K.et al.: "PACAP causes rapid Ca^<2+> release from intracellilar stores and long lasting Ca^<2+> influx mediated by Na^+ influx-dependent membrane depolarization in bouine adrenal chromsffin cells" Endocrinology. (in press).

[Publications] Funabashi,T.et al.: "Changes in preproenkephalin messenger RNA leuel in the rat ventromedial hypothalamus during the estrous cycle" Molecular Brain Research. 28. 129-134 (1995)

[Publications] Kumamoto,K.et al.: "Oxytocin-producing and vasopressin-producing eosinophils in the mouse spleen : immunohistochemical immuno-electron-microscopic and in situ hykrization studies" Cell Tissue Research. 281. 1-10 (1995)

[Publications] Ueda,S.et al.: "Role of neuropeptide Y projection on the development of serotonergic innervation in the suprachiasmatic nucleus of the rat,shown by tliple intraocular grafts" Brain Research. 673. 325-330 (1995)

[Publications] Yamashita,H.et al.: "Neurohypophysis : Recent Progress of Vasopressin and Oxytocin Research" Elsevier Science B.V., 197-204 (1995)

[Publications] 大坂寿雅 et al.: "THERAPEUTIC RESEARCH" ライフサイエンス出版株式会社, 32-35 (1995)

山下博産業医科大学, 医学部, 教授 (00030841)