1996 Fiscal Year Annual Research Report

生体工学的原理による血管壁細胞の機能発現と組織化、及び臓器工学的応用

Research Project

Project/Area Number	07044303
Research Institution	National Cardiovascular Center Research Institute
Principal Investigator	松田武久国立循環器病センター研究所, 生体工学部, 部長 (60142189)
Co-Investigator(Kenkyū-buntansha)	佐藤伸一京都府立医科大学, 医学部, 講師 (30178735) 中山泰秀国立循環器病センター研究所, 生体工学部, 室員 (50250262) 増田道隆国立循環器病センター研究所, 循環器形態部, 室長 (00190364) 藤原敬己国立循環器病センター研究所, 循環器形態部, 部長 (10190092)
Keywords	力学的ストレス / 組織工学 / 人工血管 / 人工心臓 / 細胞
Research Abstract	今年度は前年度の研究を続けて遂行し、力学的ストレスが与える効果を分子レベルから細胞レベル及び組織レベルまでを時系列的且つ時空間的に分割・定量化すると共に、各レベル間の連携による組織化過程を統一的に解釈する。次の各項目についての理論的考察及び臓器工学ハイブリッド人工血管及び内皮化人工心臓の開発の基盤への応用に際しての合目的な指針を与えた。 1)ハイブリッド血管壁組織の力学的及び構造的リモデリング過程;ハイブリッド中膜の細胞及びマトリックスの配向と階層性人工血管:上記の力学的ストレス負荷によって形成した高秩序・高密度・高配向性の円筒状ハイブリッド組織の微細形態及び力学的強度発現をしらべる。さらに、移植した人工血管の細胞及びマトリックス・レベルでの秩序性及び再構築性をしらべた。 2)流れ感知機構の分子論的原理の提出;流れ感知機構と細胞運動性:既に当研究で開発した流れ負荷装置を用いて細胞の運動性(運動方向性及び移動速度、運動軌跡)を剪断応力の関数として定量化する。膜裏打ち蛋白の解析と抗体作成。各力学的ストレス場における細胞機能動態の解析:圧力、流体力学剪断応力、引張り応力を負荷した状態の細胞機能(接着因子発現、アンジオテンシン変換酵素系等)をストレスの強度と負荷時間との関係を確立した。 3)内皮化人工心臓:内皮化人工心臓の内皮細胞層の接着力の増大を人工細胞外マトリックスの最適設計により達成した。低拍出量の状況下での非血栓性を検証した。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] N.Saito.T.Sugawara,T.Matsuda: "Synthesis and Hydrophilicity of Multifunctionally Hydroxylated Poly (acrylamides)" Macromolecules. 29. 313-319 (1996)
[Publications] H.Kita,T.Matsuda: "Biocompatible Coatings for Luminal and Outer Surfaces of Small-Caliber Artificial Grafts" Journal of Biomedical Materials Research. 30. 321-330 (1996)
[Publications] J.Hirai,T.Matsuda: "Venous Reconstruction Using Hybrid Vascular Tissue Composed of Vascular Cells and Collagen : Tissue Rageneration Process" Cell Transplantation. 5. 93-105 (1996)
[Publications] K.Doi,Y.Nakayama,T.Matsuda: "Novel compliant and Tissue-Permeable Microporous Polyurethane Vascular Prosthesis Fabricated Using and Excimer Laser Ablation Technique" Journal of Biomedical Materials Research. 31. 27-33 (1996)
[Publications] T.Matsuda,Y.Nakayama: "Surface microarchitectural design in biomedical applications : In VITRO transmural endothelialization on microporous Segmented ployurethane films fabricated using on excimer laser" Journal of Biomedical Materials Research. 31. 235-242 (1996)
[Publications] M.Tanahashi,T.Kokubo,T.Matsuda: "Quantitative assessment of apatite formation via a biomimetic method using quartz crystal microbalance" Journal of Biomedical Materials Research. 31. 243-249 (1996)
[Publications] T.Matsuda,T.Sugawara: "Synthesis of Multifunctional,Nonionic Vinyl Polymers and Their ^<13>C Spin-Lattice Relaxation Times in Deuterium Oxide Solutions" Macromolecules. 29. 5375-5383 (1996)
[Publications] T.Sugawara,T.Matsuda: "Synthesis of phenylazido-derivatized substances and photochemical surface modification to immobilize functional groups" Journal of Biomedical Materials Research. 32. 157-164 (1996)
[Publications] Y.Nakayama,T.Matsuda: "Excimer laser-induced surface fixation of polymer and its patterning" J.Appl.Phys.80. 505-508 (1996)
[Publications] S.Nagahara,T.Matsuda: "Hydrogel formation via hybridization of oligonucleotides derivatized in water-soluble vinyl polymers" Polymer Gel and Networks. 4. 111-127 (1996)
[Publications] T.Matsuda,N.Saito,T.Sugawara: "Ceric-Ion-initiating surface graft polymerization with regional control and dimensional precision" Macromolecules. 29. 7446-7451 (1996)
[Publications] Y.Nakayama,T.Matsuda: "Surface macromolecular architectual designs using photo-graft copolymerization based on photochemistry of Benzyl N,N-diethyldithiocarbamate" Macromolecules. 29. 8622-8630 (1996)
[Publications] S.Nagahara,T.Matsuda: "Cell-substrate and cell-cell interactions differently regulate cytoskeletal and extracellular matrix protein gene expression" Journal of Biomedical Materials Research. 32. 677-686 (1996)
[Publications] T.Matsuda,T.Sugawara: "Control of cell adhesion,migration,and orientation on photochemically microprocessed surfaces" Journal of Biomedical Materials Research. 32. 165-173 (1996)
[Publications] K.Doi,S.Satoh,T.Oka,T.Matsuda: "Impregnation of basic fibroblast growth factor on a microporous small caliber graft enhances vascularization" ASAIO Journal. 42(5). M394-M398 (1996)
[Publications] K.Takamizawa,S.Niu,T.Matsuda: "Mathematical simulation of unidirectional tissue formation : in vitro transanastomotic endothelialization model" Journal of Biomaterials Sience,Polymer Edition. 8(4). 323-324 (1997)

1996 Fiscal Year Annual Research Report

生体工学的原理による血管壁細胞の機能発現と組織化、及び臓器工学的応用

Principal Investigator

松田 武久 国立循環器病センター研究所, 生体工学部, 部長 (60142189)

Research Products

[Publications] N.Saito.T.Sugawara,T.Matsuda: "Synthesis and Hydrophilicity of Multifunctionally Hydroxylated Poly (acrylamides)" Macromolecules. 29. 313-319 (1996)

[Publications] H.Kita,T.Matsuda: "Biocompatible Coatings for Luminal and Outer Surfaces of Small-Caliber Artificial Grafts" Journal of Biomedical Materials Research. 30. 321-330 (1996)

[Publications] J.Hirai,T.Matsuda: "Venous Reconstruction Using Hybrid Vascular Tissue Composed of Vascular Cells and Collagen : Tissue Rageneration Process" Cell Transplantation. 5. 93-105 (1996)

[Publications] K.Doi,Y.Nakayama,T.Matsuda: "Novel compliant and Tissue-Permeable Microporous Polyurethane Vascular Prosthesis Fabricated Using and Excimer Laser Ablation Technique" Journal of Biomedical Materials Research. 31. 27-33 (1996)

[Publications] T.Matsuda,Y.Nakayama: "Surface microarchitectural design in biomedical applications : In VITRO transmural endothelialization on microporous Segmented ployurethane films fabricated using on excimer laser" Journal of Biomedical Materials Research. 31. 235-242 (1996)

[Publications] M.Tanahashi,T.Kokubo,T.Matsuda: "Quantitative assessment of apatite formation via a biomimetic method using quartz crystal microbalance" Journal of Biomedical Materials Research. 31. 243-249 (1996)

[Publications] T.Matsuda,T.Sugawara: "Synthesis of Multifunctional,Nonionic Vinyl Polymers and Their ^<13>C Spin-Lattice Relaxation Times in Deuterium Oxide Solutions" Macromolecules. 29. 5375-5383 (1996)

[Publications] T.Sugawara,T.Matsuda: "Synthesis of phenylazido-derivatized substances and photochemical surface modification to immobilize functional groups" Journal of Biomedical Materials Research. 32. 157-164 (1996)

[Publications] Y.Nakayama,T.Matsuda: "Excimer laser-induced surface fixation of polymer and its patterning" J.Appl.Phys.80. 505-508 (1996)

[Publications] S.Nagahara,T.Matsuda: "Hydrogel formation via hybridization of oligonucleotides derivatized in water-soluble vinyl polymers" Polymer Gel and Networks. 4. 111-127 (1996)

[Publications] T.Matsuda,N.Saito,T.Sugawara: "Ceric-Ion-initiating surface graft polymerization with regional control and dimensional precision" Macromolecules. 29. 7446-7451 (1996)

[Publications] Y.Nakayama,T.Matsuda: "Surface macromolecular architectual designs using photo-graft copolymerization based on photochemistry of Benzyl N,N-diethyldithiocarbamate" Macromolecules. 29. 8622-8630 (1996)

[Publications] S.Nagahara,T.Matsuda: "Cell-substrate and cell-cell interactions differently regulate cytoskeletal and extracellular matrix protein gene expression" Journal of Biomedical Materials Research. 32. 677-686 (1996)

[Publications] T.Matsuda,T.Sugawara: "Control of cell adhesion,migration,and orientation on photochemically microprocessed surfaces" Journal of Biomedical Materials Research. 32. 165-173 (1996)

[Publications] K.Doi,S.Satoh,T.Oka,T.Matsuda: "Impregnation of basic fibroblast growth factor on a microporous small caliber graft enhances vascularization" ASAIO Journal. 42(5). M394-M398 (1996)

[Publications] K.Takamizawa,S.Niu,T.Matsuda: "Mathematical simulation of unidirectional tissue formation : in vitro transanastomotic endothelialization model" Journal of Biomaterials Sience,Polymer Edition. 8(4). 323-324 (1997)

松田武久国立循環器病センター研究所, 生体工学部, 部長 (60142189)