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1997 Fiscal Year Final Research Report Summary

Studies on the cellular and molecular mechanism of neuron network construction

Research Project

Project/Area Number 07045057
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionUniversity-to-University Cooperative Research
Research Field Neurochemistry/Neuropharmacology
Research InstitutionTokyo University of Science

Principal Investigator

IKEKITA Masahiko  Science university of Tokyo, Associate Professor, 理工学部, 助教授 (70138981)

Co-Investigator(Kenkyū-buntansha) VINCENDON Guy  Univ.Louis Pasteur, Center De, Professor Neurochinie du CNRS, Centre De Neurochimie, Professor
KOJIMA Shuuji  Science university of Tokyo, Associate Professor, 生命科学研究所, 講師 (90119579)
TASHIRO Fumio  Science university of Tokyo, Professor, 基礎工学部, 教授 (70089332)
TOMOOKA Yasuhiro  Science university of Tokyo, Associate Professor, 基礎工学部, 助教授 (10197949)
ARAI Takao  Science university of Tokyo, Professor, 理工学部, 教授 (60107422)
MERSEL Mirsial  Univ.Louis Pasteur, Center De, Professor Neurochinie du CNRS
ZANETTA Jean P  Univ.Louis Pasteur, Center De, Professor Neurochinie du CNRS
Project Period (FY) 1995 – 1997
Keywordsneuron / glycoconjugate / central nerve neuronal cell / lectin / neuronal cell death / nerve growth factor / neuritegenesis / microtubule
Research Abstract

1) Many (>100) kinds of neuronal calls were cloned.
2) Cell surface Asparagine-liked oligosaccharides were analyzed by using cerebrum granule cell, designated A-40 cell.
3) Monoclonal antibodies against Asparagine-linked oligosaccharides were produced.
4) The involvement between Asparagine-linked oligosaccharides and central nervous system were analyzed by using monoclonal antibodies against Asparagine-linked oligosaccharides.
5) Neuronal cell death in brain development was analyzed by electron-microscope.
6)Two Asparagine-linked oligosaccharides, designated N-1 (5Mannose+2N-Acetylglucosamine) and N-2 (3Mannose+4N-Acetylglucosamine) were altered the representation as murine cerebrum development.
7) Stabilizing the micro-tubles was made by Ephi-tubulin in the brain development.
8) Development of cerebrum granule cell was produced by cell surface Asparagine-linked oligosaccharides.
9) Brain lectin activities were reduced in Multiple screlosis.
These results were presented at the Japanese Membrane Association Meeting (1996.5), the Japanese Biochemistry Association Meeting (1996.8 and 1997.9), the Japanese Pharmaceutical Association Meeting (1997.3 and 1998.3), the Japanese Cell Biology Association Meeting (1997.10), the International Glycobiology Association Meeting (1996.7), the International Complex Carbohydrate Association Meeting (1997.9).

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Masahiko Ikekita et al.: "Structural Alteration of Cell Surface oligosaccharides on HL-60RG Cells undergoing Apoptosis:" Research Commun.Biochem.and Cell & Molecular Biol.1・4. 249-262 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiko Ikekita et al.: "Strucural Characterization of Sialylated and Neutral N-linked Oligosaccharides Labeled with p-Aminobenzoic Acid Octyl Ester by High Performance Liquid Chromatography and Fast Atom Bombardment Mass Spectrometry:" Research Commun.Biochem.and Cell & Molecular Biol.1・4. 285-303 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiko Ikekita et al.: "Suppressive Effect of Tetrathydrobiopterin on L-b-(3,4Dihydrozyphenyl)alanine-Induced Cytoxicity in PC-12 Cells:" Pteridines. 8. 10-16 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiko Ikekita et al.: "Subcutaneous Arginine Administration Causes Apoptosis of Rat Pancreas:" Pancreas,14(3). 14・ 3. 316-318 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiko Ikekita et al.: "Secondary Structure of Hog thyroglobulins with Different Iodine Contents:" Research Commun:Biochem.and cell & Molecular Biol.1(2-3). 171-184 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiko Ikekita et al.: "Carbohydrate Analysis of Porcine Thyroglobulin Isoforma with Different Iodine Content:" Biochem.Biophys.Acta. 1336. 557-565 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池北 雅彦 他: "バイオ医療が人間を変える" PHP研究所, 262 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池北 雅彦 他: "糖鎖学概論" 丸善株式会社, 142 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeomi Murata, Orie Yasuda, Tetsuya Shimada, Takahisa Shinomiya, Shyoichi Yasuno, Takashi Yamaguchi, Seiichi Tanuma and Masahiko Ikekita: "Structural Alteration of Cell Surface oligosaccharides on HL-60RG Cells undergoing Apoptosis" Research Commun.Biochem.and Cell & Molecular Biol.(1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeomi Murata, Masayochi Shibtsuji, Yohji Yoshimi, Hiroshi Hara, Shoichi Yasuno, Takashi Yamaguchi, Hiroshi Moriya, Chales C.Sweeley, and Masahiko Ikekita: "Structural Characterization of Sialylated and Neutral N-linked Oligosaccharides Labeled with p-Aminobenzoic Acid Octyl Ester by High Performance Liquid Chromatography and Fast Atom Bombardment Mass Spectrometry" Research Commun.Biochem.and Cell & Molecular Biol. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shuji Kojima, HIyoshi Kugenuma, Sayaka Oyobe, Hiroyuki Iizuka and Masahiko Ikekita: "Suppressive Effect of Tetrahydrobiopterin on L-b-(3,4-Dihydroxyphenyl) alanine-Induced Cytoxicity in PC-12 Cells" Pteridines. 8. 10-16 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Otani, M.Ikekita, S.Tanuma, T.Sakamoto, Y.Hashimoto, A.Kuroda, M.Makuuchi: "Subcutaneous Arginine Administration Causes Apoptosis of Rat Pancreas" Pancreas. 14 (3). 316-318 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirofumi Yajima, Tadahiro Ishii, Masahiko Ikekita, Kikuo Takatera, Tadashi Watanabe, Masashi Sonoyama and Osamu Tarutani: "Secondary structure of Hog Thyroglobulins with Different Iodine Contents." Research Commun : Biochem.and Cell & Molecular Biol.1 (2-3). 171-184 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Ikekita, Hirofumi Yajima, Takeomi Murata, Kikuo Takatera, Tadashi Watanabe, Masashi Sonoyama, Yoshihiko Tezuka, Tadahiro Ishii and Osamu Tarutani: "Carbohydrate Analysis of Porcine Thyroglobulin Isoforms with Different Iodine Content" Biochem.Ciophys.Acta. 1336. 557-565 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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