1996 Fiscal Year Annual Research Report

ヒト癌の発生,進展に関与する遺伝子異常の解析

Research Project

Project/Area Number	07272204
Research Institution	Tohoku University
Principal Investigator	堀井明東北大学, 医学部, 教授 (40249983)
Keywords	癌抑制遺伝子 / 癌遺伝子 / 膵癌 / 子宮内膜癌 / DNAミスマッチ修復異常 / TGFBβレセプターII型 / IGFIIレセレプター / hMSH2遺伝子
Research Abstract	1.膵臓に関しては,comparative genomic hybridization (CGH)法,fluorescence in sity hybridization (FISH)法,マイクロサテライトマーカーを用いたPCR法により染色体領域のコピー数の異常を指標に癌遺伝子・癌抑制遺伝子の局在の候補領域を検索し,6か所の高頻度の欠失領域(1p, 6q, 9p, 12q, 17p, 18q)と2か所の増幅領域(8q, 20q)を検出した。そのうち,6q, 12q, 20qにおいては,さらに詳細に検討を加え,12qでは1cM以内の共通欠失領域を同定した。 2.子宮体癌についてもCGH法,FISH法,マイクロサテライトマーカーを用いたPCR法により検討を加え,第10番染色体長腕に2か所の高頻度の染色体欠失領域を発見した。さらに,そのうちの1か所について詳細な検討を加え,共通欠失領域を790kb以下にまでせばめることができた。 3.DNAミスマッチ修復異常の下流に位置する遺伝子異常としてtransforming growth factor (TGF)β receptor type II (RII)遺伝子とinsulin-like growth factor II receptor (IGFIIR)遺伝子における異常の検索を行い,大腸癌,胃癌ではRIIとIGFIIRの両方の異常がみられ,子宮体癌ではIGFIIRの異常がみられるが,膵癌では異常はみられないことを明らかにした。 4.hMSH2遺伝子のsplicingのバリエーションが2種類あり,合計3種類のmRNAが正常組織で発現していること,また,バリアントの発現量は低いことを明らかにした。さらに,それらのうち1種類ではsplicingのdonor siteとacceptor siteがそれぞれTAとTTであった。

Research Products

(11 results)

All Other

All Publications (11 results)

[Publications] Ouyang, H.: "The insulin-like growth factor II receptor (IGFIIR) gene is mutated in genetically unstable cancers of the endometrium, stomach and colorectum." Cancer Res.(in press).
[Publications] Nagase, S.: "Identification of a 790 kb region of common allelic loss in Chromosome 10q25-q26 in human endometrial cancer." Cancer Res.(in press).
[Publications] Fukushige, S.: "Frequent Gain of copy number on the long arm of chromosome 20 in human pancreatic adenocarcinoma." Genes Chromosom. Cancer. (in press).
[Publications] Mori, Y.: "Alternative splicing of hMSH2 in normal hman tissues." Hum. Genet.(in press).
[Publications] Horii, A.: "Genetic alterations in human pancreatic cancer." Recent advances in gastroenterological carcinogenesis I. 231-238 (1996)
[Publications] Kimura, M.: "Identification of a 1-cM region of common allelic loss in chromosome bands 12q22-q23.1 in hman pancreatic adenocarcinoma." Recent advances in gastroenterological carcinogenesis I. 733-737 (1996)
[Publications] Nagase, S.: "Deletion mapping on chromosome 10q25-26 in human endometrialcancer." Br. J. Cancer. 74. 1979-1983 (1996)
[Publications] Abe, T.: "The somatic mutation frequency of the transforming growth factor β recepter type II gene varies widely among different cancers with microsatellite instability." Eur. J. Surg. Oncol.22. 474-477 (1996)
[Publications] Kimura, M.: "Detailed deletion mapping on chromosome arm 12q in human pancreatic adenocarcinoma : identification of a 1-cM region of common allelic loss." Genes Chromosom. Cancer. 17. 88-93 (1996)
[Publications] Kazama, T.: "Proliferation of macrophage-lineage cells in the bone marrow, severe thymic atrophy, and extramedullary hematopoiesis of possible donor origin in an autopsy case of posttransplantation graft-versus-host disease." Bone Marrow Transplant. 18. 437-441 (1996)
[Publications] Sasaki, S.: "Somatic mutations of a human mismatch repair gene, hMLH1, in tumors from patients with multiple primary cancers." Hum. Mutation. 7. 275-278 (1996)

1996 Fiscal Year Annual Research Report

ヒト癌の発生,進展に関与する遺伝子異常の解析

Principal Investigator

堀井 明 東北大学, 医学部, 教授 (40249983)

Research Products

[Publications] Ouyang, H.: "The insulin-like growth factor II receptor (IGFIIR) gene is mutated in genetically unstable cancers of the endometrium, stomach and colorectum." Cancer Res.(in press).

[Publications] Nagase, S.: "Identification of a 790 kb region of common allelic loss in Chromosome 10q25-q26 in human endometrial cancer." Cancer Res.(in press).

[Publications] Fukushige, S.: "Frequent Gain of copy number on the long arm of chromosome 20 in human pancreatic adenocarcinoma." Genes Chromosom. Cancer. (in press).

[Publications] Mori, Y.: "Alternative splicing of hMSH2 in normal hman tissues." Hum. Genet.(in press).

[Publications] Horii, A.: "Genetic alterations in human pancreatic cancer." Recent advances in gastroenterological carcinogenesis I. 231-238 (1996)

[Publications] Kimura, M.: "Identification of a 1-cM region of common allelic loss in chromosome bands 12q22-q23.1 in hman pancreatic adenocarcinoma." Recent advances in gastroenterological carcinogenesis I. 733-737 (1996)

[Publications] Nagase, S.: "Deletion mapping on chromosome 10q25-26 in human endometrialcancer." Br. J. Cancer. 74. 1979-1983 (1996)

[Publications] Abe, T.: "The somatic mutation frequency of the transforming growth factor β recepter type II gene varies widely among different cancers with microsatellite instability." Eur. J. Surg. Oncol.22. 474-477 (1996)

[Publications] Kimura, M.: "Detailed deletion mapping on chromosome arm 12q in human pancreatic adenocarcinoma : identification of a 1-cM region of common allelic loss." Genes Chromosom. Cancer. 17. 88-93 (1996)

[Publications] Kazama, T.: "Proliferation of macrophage-lineage cells in the bone marrow, severe thymic atrophy, and extramedullary hematopoiesis of possible donor origin in an autopsy case of posttransplantation graft-versus-host disease." Bone Marrow Transplant. 18. 437-441 (1996)

[Publications] Sasaki, S.: "Somatic mutations of a human mismatch repair gene, hMLH1, in tumors from patients with multiple primary cancers." Hum. Mutation. 7. 275-278 (1996)

堀井明東北大学, 医学部, 教授 (40249983)