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1998 Fiscal Year Final Research Report Summary

Channel-Transporter Correlation

Research Project

Project/Area Number 07276103
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionOkazaki National Research Institutes

Principal Investigator

OKADA Yasunobu  National Institute for Physiological Sciences, Professor, 生理学研究所, 教授 (10025661)

Co-Investigator(Kenkyū-buntansha) MARUMO Fumiaki  Tokyo Med. Dent. Univ., Professor, 医学部, 教授 (00050443)
TSUCHIYA Tomofusa  Okayama University, Professor, 薬学部, 教授 (80012673)
UEDA Kazumitsu  Kyoto University, Research Associate, 農学部, 助手 (10151789)
IMAI Masashi  Jichi Medical University, Professor, 教授 (40049010)
TAKEGUCHI Noriaki  Toyama Med. Pharmac. Univ., Professor, 薬学部, 教授 (00019126)
Project Period (FY) 1995 – 1998
Keywordsion channel / water channel / transporter / Ca pump / structure-function relation / cloning
Research Abstract

Structural and functional correlations of channels and/or transporters were investigated from physiological, molecular biological and biochemical points of view. During these three years in this study, new channels (over 29) and new transporters (over 42) were discovered, and their genes were cloned. Structure-function correlations were elucidated by identification of numbers of functional domains and amino acid sites. New types of functional or structural correlation between channels and transporters were also demonstrated (over 10). New diseases were identified to be due to mutations of genes of channels or transporters (over 10). Especially a big progress was achieved in the research of ATP-binding cassette (ABC) transporter proteins. For example, a novel ABC protein cMOAT was cloned and found to mediate the ATP-dependent transport of glutathion-conjugates. The expression of cMOAT was found to be defective in the Dubin-Johnson syndrome. The ATP-sensitive KィイD1+ィエD1 channel was revealed to be a complex of two subunits : SUR1 of the ABC protein family and Kir6.2 of the inward-rectifier KィイD1+ィエD1 channel family. Some of ABC proteins have been found to have more than one activities of transporter, channel and their regulator. For example, P-glycoprotein and CFTR were found to be a regulator for volume-sensitive ClィイD1-ィエD1 channel and ATP release, respectively.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Tsumura,Hazama & Okada: "Activation of cAMP-dependent CI corrents in guinea-pig Paneth cells without relevant evidence for CFTR expression."Journal of Physiology. 512・3. 765-777 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zhou,Hazama & Okada: "Tyrosine kinase-independent extracellular action of genistein on the CFTR CI channel in guinea pig ventricular myocytes and CFTR-transfected mouse fibroblasts."Japanese Journal of Physiology. 48・5. 389-396 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sabirov,Morishima & Okada: "Probing the water permeability of ROMK1 and amphotericin B channels using Xenopus oocytes."Biochimica Biophysica Acta. 1368. 19-26 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡田泰伸: "CIチャネルと細胞容積調節および細胞死"Mebio. 15・6. 61-68 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 森島 繁、岡田泰伸: "イオンチャネルの構造と機能"Mebio. 15・6. 12-24 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡田泰伸、小島 至: "チャネルとトランスポータの構造と疾患"Molecular Medicine. 35. 4-17 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Okada: "Cell Volume Regulation:The Molecular Mechanism and Volume Sensing Machinery"Elsevier,Amsterdam. 214 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Okada: "Cell Volume Regulation(Ed.F.Lang)"Karger,Basel. 12

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R. Z. Sabirov, S. Morishima & Y. Okada: "Probing the water permeability of ROMK1 and amphotericin B channels using Xenopus oocytes"Biochem. Biophys. Acta. 1368. 19-26 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Nakamura, S. Arii, K. Monden, M. Furutani, Y. Takeda, M. Imamura, M. Tominaga & Y. Okada: "Expression of the Na/Ca exchanger emerges in hepatic stellate cells after activation in association with liver fibrosis"Proc. Natl. Acad. Sci. USA. 95. 5389-5394 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Liu, S. Oiki, T. Tsumura, T. Shimizu & Y. Okada: "Glibenclamide blocks volume-sensitive ClィイD1-ィエD1 channels by dual mechanisms"Am. J. Physiol.. 275. C343-C351 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Okada, S. Oiki, A. Hazama & S. Morishima: "Criteria for the molecular identification of the volume-sensitive outwardly rectifying ClィイD1-ィエD1 channel"J. Gen. Physiol.. 112. 1-3 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Higuchi, S. Suga, T. Tsuchiya, H. Hisada, S. Morishima, Y. Okada & M. Maeshima: "Molecular cloning, water channel activity and tissue specific expression of two isomers of radish vacuolar aquaporin"Plant Cell Physiol.. 39. 905-913 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Tsumura, A. Hazama, T. Miyoshi, S Ueda & Y. Okada: "Activation of cAMP-dependent ClィイD1-ィエD1 currents in guinea-pig Paneth cells without relevant evidence for CFTR expression"J. Physiol. (London). 5212. 765-777 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Hazama, S. Hayashi & Y. Okada: "Cell surface measurements of ATP release from single pancreatic β cells using a novel biosensor technique"Pflugers Arch. Eur. J. Physiol.. 437. 31-35 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. -S. Zhou, A. Hazama & Y. Okada: "Tyrosine kinase-independent extracellular action of genistein on the CFTR ClィイD1-ィエD1 channel in guinea pig ventricular myocytes and CFTR-transfected mouse fibroblasts"Jpn. J. Physiol.. 48. 389-396 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Okada: "Cell Volume regulation : The molecular Mechanism and Volume Sensing Machinery"Elsevier, Amsterdam. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Okada: "Cell volume-sensitive chloride channel. In, "Cell Volume Regulation" (ed. F. Lang)"Karger, Basel. (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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