1997 Fiscal Year Annual Research Report

高等植物における雌雄の分化・受粉過程の遺伝解剖的吟味

Research Project

Project/Area Number	07281102
Research Institution	Tohoku University
Principal Investigator	日向康吉東北大学, 農学部, 名誉教授 (00005589)
Co-Investigator(Kenkyū-buntansha)	長戸康郎東京大学, 大学院農学生命科学, 教授 (10143413) 鎌田博筑波大学, 生物, 教授 (00169608) 鳥山欽哉東北大学, 農学部, 助教授 (20183882) 米田好文北海道大学, 大学院・理学研究科, 教授 (10124215) 磯貝彰奈良先端科学技術大学, バイオ, 教授 (20011992)
Keywords	高等植物 / 雌雄性 / 受粉 / 受精 / 花粉 / 花 / 自家不和合性 / 胚
Research Abstract	研究目的:高等植物における雌雄両性の分化・成熟過程と受粉・受精過程の2つに焦点を絞り、それらに関する遺伝子を解剖吟味し、植物の有性生殖機構の解明を目指している。研究成果の概要:特に注目すべき研究成果は以下の通りである。(1)RAPD法やYACを利用したアサ、メランドリウムの性染色体の解析、(3)ウリ類の雌雄性決定に関わる遺伝子の単離、(3)イネやシロイヌナズナの突然変異体を利用した花器官の形成機構の解明、(4)減数分裂特異的遺伝子の解析、(5)花粉アレルゲン遺伝子など花粉成熟に関わる遺伝子の解析、(6)ミトコンドリア遺伝子と細胞質雄性不捻性の機構解明、(7)アブラナ科自家不和合性に関するS座位およびS多重遺伝子のクローニング、およびFISHによるS遺伝子の同定、(8)S-RNase型自家不和合性に関与するS-RNaseのX線結晶解析、(9)サツマイモの自家不和合性に関与する新遺伝子の解析、(10)雄性配偶体に特異的に発現する遺伝子の解析、(11)イネの突然変異体を用いた初期胚発生の解析、(12)ニンジン培養細胞の不定胚を用いた初期胚発生の解析。これらの課題に関して、約190報の論文を発表し、また3件の特許を申請するなど、多くの成果を得ている。研究組織と研究班の連携状況:2回の総括班会議を開催し、研究の進展状況を評価・検討した。News and Viewsを10号まで発刊して情報の伝達に務める一方、A班とB班の合同研究会を2回、また、合同シンポジウム、学会の際のシンポジウム、ワークショップ、若手研究者の会を各1回開催して、相互の研究の活性化につとめている。これらの機会を利用した情報交換が活発で、20件を越える共同研究が行われており、その成果も多数発表されている。

Research Products
(18 results)

All Other

All Publications (18 results)

[Publications] Suzuki,G., Watanabe,M., Isogai,A. and Hinata,K.: "Highly conserved 5'-flanking regions of two self-incompatibility genes,SLG9 and SRK9." Gene. 191. 123-126 (1997)
[Publications] Takasaki,T., Hatakeyama,K., Ojima,K., Watanabe,M., Toriyama,K. and Hinata,K.: "Factors influencing Agrobacterium-mediated transformation of Brassica rapa L." Breed.Sci.47. 127-134 (1997)
[Publications] Suzuki,G., Watanabe,M., Toriyama,K., Isogai,A. and Hinata,K.: "Direct cloning of the Brassica S locus by using a Pl-derived artificialchromosome (PAC) vector." Gene. 199. 133-137 (1997)
[Publications] Suzuki,G., Watanabe,M., Kai,N., Matsuda,N., Toriyama,K., Takayama,S., Isogai,A. and Hinata,K.: "Three members of the S-multigene family are linked to the S locus of Bressica." Mol.Gen.Genet.256. 257-264 (1997)
[Publications] Watana,M., Ono,T., Hatakeyama,K., Takayama,S., Isogai,A. and Hinata,K.: "Molecular characterization of SLG and S-related genes in a self-compatible Brassica campersris L.var.yellow sarson." Sex.Plant Reprod.10. 332-340 (1997)
[Publications] Hanzawa,Y., Takahashi,T. and Komeda,Y.: "ACL5:an Arabidopsis gene required for the internodal elongation after flowering." The Plant Journal. 12(4). 863-874 (1997)
[Publications] Yokoi,S, Tsuchiya T, Toriyama K, Hinata K: "Tapetum-specific expression of the Osg6B promoter-beta-glucuronidase gene in transgenic rice." Plant Cell Rep.16. 363-367 (1997)
[Publications] Matuda N, Kishitani S, Toriyama K: "Antisense and sense CHS cDNA expressed by a tapetum-specific promoter causes partial male sterility in transgenic tobacco." Plant Biotechnology. 14. 157-161 (1997)
[Publications] Abe F, Kitashiba H, Kishitani S, Toriyama K: "Isolation of a cDNA clone encoding the altemative oxidase expressed in rece anthers." Sex Plant Reprod. 10. 374-375 (1997)
[Publications] Kitashiba H and Toriyama K: "Expression of a gene for a protein similar to HIV-1 Tat binding protien 1 (TBP1) in floral prgans of Brassica rapa." Plant Cell Physiol. 38. 966-969 (1997)
[Publications] A.Ojima, H.Shiota, K.Higashi, H.Kmada, Y.Shinma, M.Wada and S.Satoh: "An extracellular insoluble inhibitor of cystein proteinases in cellcultures and seeds of carrot." Plant Mol.Biol.,. 34. 99-109 (1997)
[Publications] K.Osuga, H.Kamada and A.Komamine: "Frequency improvement of somatic embryogensis at high embryo denstiy by partial replacement of medium in carrot suspension cultures." J.Fermentation and Bioengineering. 84. 275-278 (1997)
[Publications] K.Sage-Ono, M.Ono, H.Harada and H.Kamada: "Dark inducible accumulation of mRAN for a translationally controlled trmor protein (TCTP) homolog in Pharbitis nil." Plant Cell Physiol.,. (accepted). (1998)
[Publications] K.Sage-Ono, M.Ono, H.Harada and H.Kamada: "Accumulation of a clockregulated transcript during flower-inductive darkness in Pharbitis." Plant Physiol.,. (accepted). (1998)
[Publications] Takahashi,M., Nagasawa,N., Kitano,H. and Nagato,Y.: "Panicle phytomes 1 mutations affect panicle architecture of rice." Theoret.Appl.Genet.(in press). (1998)
[Publications] A.Estiati, T.Kubo, T.Mikami: "The ribosomal protein S7 gene is transcribed and edited in sugar beet mitochondria." Physiologia Plantaurum. (in press). (1998)
[Publications] M.Senda, Y.Onodera, T.Mikami: "Recombination events across the atpA-associated repeated sequences in the mitochondrial genomes of beets." Theoretical and Applied Genetics. (in press). (1998)
[Publications] M.Senda, Y.Onodera, T.Mikami: "Cytoplasmic diversity in leaf beet cultivars as revealed by mitochondrial DNA analysis." Hereditas. (in press). (1998)

1997 Fiscal Year Annual Research Report

高等植物における雌雄の分化・受粉過程の遺伝解剖的吟味

Principal Investigator

日向 康吉 東北大学, 農学部, 名誉教授 (00005589)

Research Products

[Publications] Suzuki,G., Watanabe,M., Isogai,A. and Hinata,K.: "Highly conserved 5'-flanking regions of two self-incompatibility genes,SLG9 and SRK9." Gene. 191. 123-126 (1997)

[Publications] Takasaki,T., Hatakeyama,K., Ojima,K., Watanabe,M., Toriyama,K. and Hinata,K.: "Factors influencing Agrobacterium-mediated transformation of Brassica rapa L." Breed.Sci.47. 127-134 (1997)

[Publications] Suzuki,G., Watanabe,M., Toriyama,K., Isogai,A. and Hinata,K.: "Direct cloning of the Brassica S locus by using a Pl-derived artificialchromosome (PAC) vector." Gene. 199. 133-137 (1997)

[Publications] Suzuki,G., Watanabe,M., Kai,N., Matsuda,N., Toriyama,K., Takayama,S., Isogai,A. and Hinata,K.: "Three members of the S-multigene family are linked to the S locus of Bressica." Mol.Gen.Genet.256. 257-264 (1997)

[Publications] Watana,M., Ono,T., Hatakeyama,K., Takayama,S., Isogai,A. and Hinata,K.: "Molecular characterization of SLG and S-related genes in a self-compatible Brassica campersris L.var.yellow sarson." Sex.Plant Reprod.10. 332-340 (1997)

[Publications] Hanzawa,Y., Takahashi,T. and Komeda,Y.: "ACL5:an Arabidopsis gene required for the internodal elongation after flowering." The Plant Journal. 12(4). 863-874 (1997)

[Publications] Yokoi,S, Tsuchiya T, Toriyama K, Hinata K: "Tapetum-specific expression of the Osg6B promoter-beta-glucuronidase gene in transgenic rice." Plant Cell Rep.16. 363-367 (1997)

[Publications] Matuda N, Kishitani S, Toriyama K: "Antisense and sense CHS cDNA expressed by a tapetum-specific promoter causes partial male sterility in transgenic tobacco." Plant Biotechnology. 14. 157-161 (1997)

[Publications] Abe F, Kitashiba H, Kishitani S, Toriyama K: "Isolation of a cDNA clone encoding the altemative oxidase expressed in rece anthers." Sex Plant Reprod. 10. 374-375 (1997)

[Publications] Kitashiba H and Toriyama K: "Expression of a gene for a protein similar to HIV-1 Tat binding protien 1 (TBP1) in floral prgans of Brassica rapa." Plant Cell Physiol. 38. 966-969 (1997)

[Publications] A.Ojima, H.Shiota, K.Higashi, H.Kmada, Y.Shinma, M.Wada and S.Satoh: "An extracellular insoluble inhibitor of cystein proteinases in cellcultures and seeds of carrot." Plant Mol.Biol.,. 34. 99-109 (1997)

[Publications] K.Osuga, H.Kamada and A.Komamine: "Frequency improvement of somatic embryogensis at high embryo denstiy by partial replacement of medium in carrot suspension cultures." J.Fermentation and Bioengineering. 84. 275-278 (1997)

[Publications] K.Sage-Ono, M.Ono, H.Harada and H.Kamada: "Dark inducible accumulation of mRAN for a translationally controlled trmor protein (TCTP) homolog in Pharbitis nil." Plant Cell Physiol.,. (accepted). (1998)

[Publications] K.Sage-Ono, M.Ono, H.Harada and H.Kamada: "Accumulation of a clockregulated transcript during flower-inductive darkness in Pharbitis." Plant Physiol.,. (accepted). (1998)

[Publications] Takahashi,M., Nagasawa,N., Kitano,H. and Nagato,Y.: "Panicle phytomes 1 mutations affect panicle architecture of rice." Theoret.Appl.Genet.(in press). (1998)

[Publications] A.Estiati, T.Kubo, T.Mikami: "The ribosomal protein S7 gene is transcribed and edited in sugar beet mitochondria." Physiologia Plantaurum. (in press). (1998)

[Publications] M.Senda, Y.Onodera, T.Mikami: "Recombination events across the atpA-associated repeated sequences in the mitochondrial genomes of beets." Theoretical and Applied Genetics. (in press). (1998)

[Publications] M.Senda, Y.Onodera, T.Mikami: "Cytoplasmic diversity in leaf beet cultivars as revealed by mitochondrial DNA analysis." Hereditas. (in press). (1998)

日向康吉東北大学, 農学部, 名誉教授 (00005589)