受粉・受精機構の解析

1998 Fiscal Year Annual Research Report

• Project/Area Number: 07281103
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas (A)
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: 磯貝 彰 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20011992)
• Co-Investigator(Kenkyū-buntansha): 北野 英己 名古屋大学, 大学院・生命農学研究科, 助教授 (50144184) ; 鎌田 博 筑波大学, 生物科学系, 教授 (00169608) ; 渡辺 正夫 岩手大学, 農学部, 助教授 (90240522) ; 神山 康夫 三重大学, 生物資源学部, 教授 (80024579) ; 乗岡 茂巳 大阪大学, 蛋白質研究所, 助教授 (70198638)
• Keywords: 自家不和合性 / S遺伝子 / S-RNase / S-レセプターキナーゼ / FISH / 胚発生 / 不定胚

Research Abstract

1. アブラナの自家不和合性
磯貝はBrassica rapaの花粉から、SLGに結合する物質群を単離した。一方、FDDによって、花粉で発芽し多型が認められる2種類の遺伝子群を単離した。これらはシステインに富む分子量約7kDの蛋白質であった。渡辺は、B.rapaのS9系統の76kbのSLG,SRKを含むゲノム断片中で葯・花粉で発現している2種類の遺伝子を同定した。福井はB.rapaの染色体をDNAファイバー化し、FISHによりSLGとSRKを可視化することに成功した。
2. ナシ、タバコの自家不和合性
乗岡はニホンナシS3-RNaseの結晶化に成功した。タバコSl-RNaseの水銀置換結晶については2.7オングストロームの分解能までの回折強度データを収集した。またGSTとニホンナシS3-RNaseの融合蛋白質の大腸菌での発現に成功した。
3. サツマイモの自家不和合性
神山はサツマイモ野生2倍体種において,S遺伝子型に特異的な柱頭蛋白質(SSP)を解析し、これが柱頭表面の乳頭細胞で強い発現すること、またS遺伝子から1.2cMの距離にマツピングされることを明らかにした。
4. 初期胚発生
北野はイネ初期胚発生突然変異体を用いて遺伝解析を行い、合計12の遺伝子座を同定した。変異体のホモ型胚を培養し植物体再分化の可能性を検討した。鎌田はニンジンの不定胚および受精胚において胚発生の極く初期から発現している遺伝子(C-ABI3)およびシロイヌナズナのその相同遺伝子(ABI3)のプロモーター領域に共通して存在するシス配列と思われる配列を同定した

Research Products

• [Publications] M.Iwano: "Visualization of a self-incompatibility gene in Brassica campestris L.by multicolor FISH." Theoret.Appl.Genet.96. 751-757 (1998)
• [Publications] T.Entani: "Centrimeric localization of an S-RNase gene in Petunia hybrida Vilm." Theopr.Appl.Genet.(in press). (1999)
• [Publications] K.Hatakeyama: "Dominance relationships between S-alleles in self-incompatible Brassica campestris L." Heredity. 79. 241-247 (1998)
• [Publications] K.Toriyama: "Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brasscia rapa." FEBS Lett.424. 234-238 (1998)
• [Publications] K.Hatakeyama: "Molecular characterization of S locus genes,SLG and SRK,in a pollen-recessive self-incompatibility haplotype of Brassica rapa L." Genetics. 149. 1587-1597 (1998)
• [Publications] Y.Sasaki: "Localization of an SLG protein expressed under the regulation of a tapetum-specific promoter in anthers of transgenic Brassica napus." Sex.Plant Reprod.11. 245-250 (1998)
• [Publications] K.Hatakeyama: "High sequence similarity between SLG and the receptor domain of SRK is not necessarily involved in higher dominance relationships in stigma in self-incompatible Brassica rapa L." Sex.Plant Reprod.11. 292-294 (1998)
• [Publications] M.Watanabe: "Sequence comparison of four SLR1 alleles in Brassica campestris(syn.rapa)." Sex.Plant Reprod.11. 295-296 (1998)
• [Publications] K.Sasaki: "Detection of glycoproteins in carrot somatic embryos induced by 2,4-D or stress treatment." Plant Biotechnology. 15. 241-243 (1998)
• [Publications] K.Higashi: "Inhibitory conditioning for carrot somatic embryogenesis in high cell density cultures." Plant Cell Rep.18. 2-6 (1998)
• [Publications] H.Shiota: "C-ABI3,the carrot homologue of the Aradibopsis ABI3 gene,is expressed during both zygotic and somatic embryogenesis and functions in the regulation of embryo-specific ABA-inducible genes." Plant Cell Physiol.39. 1184-1193 (1998)
• [Publications] T.Kiyosue: "A chromo box gene from carrot(Daucus carota L.):its cDNA structure and expression during somatic and zygotic embryogenesis." BBA. 1398. 42-46 (1998)
• [Publications] Y.Tachikawa: "Changes in protein pattern during stress-induction of carrot(Daucus carota L.)somatic embryogenesis." Plant Bioptechnology. 15. 17-22 (1998)
• [Publications] K.Higashi: "Patterns of expression of glutamine synthetase isoformes during somatic and zygotic embryogenesis in carrot." Plant Cell Physiol.39. 418-424 (1998)
• [Publications] K.Sage-Ono: "Molecular identification of two genes of germin-like protein in Arabidopsis." Plant Biotechnology. 15. 103-108 (1998)
• [Publications] K.Sage-Ono: "Accumulation of a clock-regulated transcript during flower-inductive darkness in Pharbitis." Plant Physiol.116. 1479-1485 (1998)
• [Publications] K.Sage-Ono: "Dark inducible accumulation of mRNA for a transcriptionally controlled tumor protein(TCTP)homolog in Pharbitis nil." Plant Cell Physiol.39. 357-360 (1998)
• [Publications] K.Sakamoto: "Characterization; Genome sizes and morphology of sex chromosomes in hemp(Canabis sativa L.)." Cytologia. 63. 459-464 (1998)
• [Publications] H.Shiota: "Successful long-term preservation of abscisic acid-treated and desiccated carrot somatic embryos." Plant Cell Rep.(in press). (1999)
• [Publications] T.Ishimizu: "S-genotype of ‘Hosui'of Japanese pear is S3S5." J.Japan.Soc.Hort.Sci.67(1). 35-38 (1998)
• [Publications] T.Ishimizu: "Primary structural features of rosaceous S-RNases associated with gametophytic self-incompatibility." Plant Mol.Biol.37. 931-941 (1998)
• [Publications] T.Ishimizu: "Identification of regions in which positive selection may operate in S-RNase of Maloideae in Rosaceae: Imptication for S-allele-specific recognition sites in S-RNase." FEBS Letters. 440. 337-342 (1998)
• [Publications] D.Kiyozumi: "Molecular cloning and nucleotide sequencing of a cDNA encoding UDP-glucose pyrophosphorylase of Japanese pear(Pvrus pyrifolia Nakai)" Plant Physiology. 119. 363 (1999)
• [Publications] Y.Inukai: "Genetic analysis of induced rice developmental mutants showing radicleless and crown rootless." R.G.N.15(in press). (1998)
• [Publications] Escalante,A.: "RFLP analysis and genomic in situ hybridization(GISH)in somatic hybrids and their progeny between Lycopersicon esculentum and Solanum lycopersicoides." Theor.Appl.Genet.96. 719-726 (1998)
• [Publications] Fukui K.: "Quantitative karyotyping of three diploid Brassica species by imaging methods and localization of 45S rDNA loci on the identified chromosomes." Theor.Appl.Genet.96. 325-330 (1998)
• [Publications] Mizukami Y.: "Agronomic and cytological characterization of F1 hybrids between Lolium multiflorum Lam.and Festuca arundinacea var.glaucescens Boiss." Grassland. 44. 14-21 (1998)
• [Publications] Mitsunaga S.: "Detection of proteins binding to the promoter regions DNA using a nonradioactive gel-retardation assay." Biosci.Biotechnol.Biochem.62. 1812-1814 (1998)
• [Publications] Rajyashri K.R.: "Isolation and FISH mapping of Yeast Artificial Chromosomes(YACs)encompassing an allele of the Gm2 gene for gall midge resistance in rice." Theor.Appl.Genet.97. 507-514 (1998)
• [Publications] Wako T.: "Comparative analysis of the topographical distribution of acetylated histone H4 using confocal microscopy and a deconvolution system." Anal.Chim.Acta. 365. 9-17 (1998)
• [Publications] Yamagishi M.: "Chromosomal regions controlling anther culturability in rice(Oryza sativa L.)." Euphytica. 103. 227-234 (1998)
• [Publications] Kato S.: "Condensation pattern analysis using a newly developed chromosome image analyzing system (CHIAS III)." Chromosome Res.6. 473-479 (1998)
• [Publications] Shishido R.: "Detection of specific chromosome reduction in rice somatic hybrids with the A,B and C genomes by multi-color genomic in situ hybridization." Theor.Appl.Genet.97. 1013-1018 (1998)
• [Publications] Ohmido N.: "Physical mapping of unique nucleotide sequences on identified rice chromosomes." Plant Mol.Biol.38. 1043-1052 (1998)
• [Publications] Kamisugi Y.: "Visualization of the Brassica self-incompatibility S-locus on identified oilseed rape chromosomes." Plant Mol.Biol.38. 1081-1087 (1998)
• [Publications] Sakamoto K.: "Characterization; Genome sizes and morphology of sex chromosomes in hemp(Cannabis sativa L.)." Cytologia. 63. 459-464 (1998)
• [Publications] 神山康夫: "植物の生殖と遺伝的多様性" 東北大学遺伝生態研究センター(印刷中), (1999)
• [Publications] 乗岡茂巳: "ニホンナシ自家不和合性とリボヌクレアーゼ -雌しべ・花粉間の自他認識-" 蛋白質工学基礎研究センターだより, 7 (1998)
• [Publications] 北野英己: "発生分化と形態形成に関わる突然変異体 根の事典" 根の事典編集委員会編 朝倉書店, 3 (1998)
• [Publications] Nagato,Y.: "Morphogenetic mutants of rice.Molecular Biology of Rice" Springer-Verlag, 21 (1998)
• [Publications] 福井希一: "染色体・ゲノム解析機器における最近の進歩.クロモソーム" ユニバーサルアカデミープレス, 179 (1998)
• [Publications] 福井希一: "植物染色体:構造とその機能的意義.クロモソーム" ユニバーサルアカデミープレス, 179 (1998)
• [Publications] 福井希一: "クロモソーム" ユニバーサルアカデミープレス, 179 (1998)
• [Publications] 福井希一: "体細胞分裂の生化学.クロモソーム" ユニバーサルアカデミープレス, 179 (1998)
• [Publications] Fukui K.: "Frontier Researches in Plant Biotechnology" Organizing Committee of Korea-Japan Symposium on Plant Biotechnology, 120 (1998)
• [Publications] Wako T.: "Frontier Researches in Plant Biotechnology" Dynamics of histone acetylation during a cell cycle in barley., 120 (1998)