Molecular Analyses of Mechanisms of Pollination and Ferulzation

1998 Fiscal Year Final Research Report Summary

• Project/Area Number: 07281103
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: ISOGAI Akira Nara Institute of Science and Technology Graduate School of Biological Sciences, Professor, バイオサイエンス研究科, 教授 (20011992)
• Co-Investigator(Kenkyū-buntansha): WATANABE Masao Iwate University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (90240522) ; KOWYAMA Yauso Mie University, Faculty of Bioresources, Professor, 生物資源学部, 教授 (80024579) ; NORIOKA Shigemi Osaka University, Institute of Protein Research, Associate Professor, 蛋白質研究所, 助教授 (70198638) ; KITANO Hidemi Nagoya University. School of Agricultural Sciences, Associate professor, 生命農学研究科, 助教授 (50144184) ; KAMADA Hiroshi University of Tsukuba, Institute of Biological Sciences, Professor, 生物科学系, 教授 (00169608)
• Project Period (FY): 1995 – 1998
• Keywords: self-incompatibility / S-gene / S-Rnase / S-receptor kinase / FISH / embryogenesis / adventitious embryo

Research Abstract

(1) Self-incompatibility of Brassica.
We have analyzed pollen extract and pollen mRNA of Brassica campestris using various biochemical and molecular biological methods and found candidate molecules of the pollen S-determinant. Further we analyzed the genomic clone including SLG and SRK. and found several genes which would he expressed especially in pollen and anther. We have succeeded in visualization of SRK and SLO genes in the DNA fibets of Brassica rapa with FISH.
(2) Self-incompatibility of Japanese pear and related plants
We have cloned and sequenced S-RNase genes of Japanese pear and further we have succeeded in obtaing crystals of S-Rnase for X-ray analysis to determine the molecular structure of S-RNase.
(3) Self-incompanbility of Ipomea
We have isolated a cDNA encoding a stigma-specific protein from wild species of Ipomea and analyzed its expression in the stigma. The gene was mapped at the distance of 1.2 cM from the S-locus.
(4) Embriogenesis
We have identified 12 loci affecting embryogenesis of rice at early stages using mutant plants. We have analyzed a gene expressed in embryo and adventitious embryo of carrot at a early stage and identified a cis sequence at the promoter region in comparison of a homologous gene of Arabidopsis.

Research Products

• [Publications] M. Iwano: "Visualization of a self-incompatibility gene in Brassica campestris L. by multicolor FISH"Theoret. Appl. Genet.. 96. 751-757 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] T. Entani: "Centrimeric localization of an S-RNase gene in Petunia hybrida Vilm"Theoret. Appl. Genet.. 99. 391-397 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K. Hatakeyama: "Dominance relationships between S-alleles in self-incompatible Brassica campestris L."Heredity. 79. 241-247 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K. Toriyama: "Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa"FEBS Lett.. 424. 234-238 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K. Hatakeyama: "Molecular characterization of S locus genes, SLG and SRK, in a pollen-recessive self-incompatibility haplotype of Brassica para L."Genetics. 149. 1587-1597 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Y. Sasaki: "Localization of an SLG protein expressed under the regulation of a tapetum-specific promoter in anthers of transgenic Brassica napus"Sex. Plant Reprod.. 11. 245-250 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Y. Tachikawa: "Changes in protein pattern during stress-induction of carrot (Daucus carota L.) somatic embryogenesis"Plant Bioptechnology. 15. 17-22 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K. Higashi: "Patterns of expression of glutamine synthetase isoformes during somatic and zygotic embryogenesis in carrot"Plant Cell Physiol.. 39. 418-424 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K. Sage-Ono: "Molecular identification of two genes of germin-like protein in Arabidopsis"Plant Biotechnology. 15. 103-108 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K. Sage-Ono: "Accumulation of a clock-regulated transcript during flower-inductive darkness in Pharbitis"Plant Physiol.. 116. 1479-1485 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K. Sage-Ono: "Dark inducible accumulation of mRNA for a transcriptionally controlled tumor protein (TCTP) homolog in Pharbitis nil"Plant Cell Physiol.. 39. 357-360 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K. Sakamoto: "Characterization ; Genome sizes and morphology of sex chromosomes in hemp (Canabis sativa L.)"Cytologia. 63. 459-464 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Escalante, A.: "RFLP analysis and genomic in situ hybridization (GISH) in somatic hybrids and their progeny between Lvcopersicon esculentum and Solanum lvsopersicoides"Theor. Appl. Genet.. 96. 719-726 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fukui, K.: "Quantitative karyotyping of three diploid Brassica species by imaging methods and localization of 45S rDNA loci on the identified chromosomes"Theor. Appl. Genet.. 96. 325-330 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mizukami Y.: "Agronomic and cytological characterization of F1 hybrids between Lolium multiflorum Lam. and Festuca arundinacea var. glaucescens Boiss"Grassland. 44. 14-21 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mitsunaga S.: "Detection of proteins binding to the promoter regions DNA using a nonradioactive gel-retardation assay"Biosci. Biotechnol. Biochem.. 62. 1812-1814 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Rajyashri K.R.: "Isolation and FISH mapping of Yeast Artificial Chromosomes (YACs) encompassing an allele of the Gm2 gene for gall midge resistance in rice"Theor. Appl. Genet.. 97. 507-514 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Wako T.: "Comparative analysis of the topographical distribution of acetylated histone H4 using confocal microscopy and a deconvolution system"Anal. Chim. Acta. 365. 9-17 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K. Hatakeyama: "High sequences similarity between SLG and the receptor domain of SRK is not necessarily involved in higher dominance relationships in stigma in self-incompatible Brassica rapa L."Sex. Plant Reprod.. 11. 292-294 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M. Watanabe: "Sequence comparison of four SLR1 alleles in Brassica campestris (syn. rapa)"Sex. Plant Reprod.. 11. 295-296 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K. Sasaki: "Detection of glycoproteins in carrot somatic embryos induced by 2,4-D or stress treatment"Plant Biotechnology. 15. 241-243 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K. Higashi: "Inhibitory conditioning for carrot somatic embryogenesis in high cell density cultures"Plant Cell Rep.. 18. 2-6 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] H. Shiota: "C-ABI3, the carrot homologue of the Aradibopsis ABI3 gene, is expressed during both zygotic and somatic embryogenesis and functions in the regulation of embryo-specific ABA-inducible genes"Plant Cell Physiol.. 39. 1184-1193 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] T. Kiyose: "A chromo box gene from carrot (Daucus carota L.) : its cDNA structure and expression during somatic and zygotic embryogenesis"BBA. 1398. 42-46 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamagishi, M.: "Chromosomal regions controlling another culturability in rice (Oryza sativa L.)"Euphytica. 103. 227-234 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kato S.: "Condensation pattern analysis using a newly developed chromosome image analyzing system (CHIAS III)"Chromosome Res.. 6. 473-479 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Shishido R.: "Detection of specific chromosome reduction in rice somatic hybrids with the A, B and C genomes by multi-color genomic in situ hybridization"Theor. Appl. Genet.. 97. 1013-1018 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ohmido N.: "Physical mapping of unique nucleotide sequences on identified rice chromosomes"Plant Mol. Biol.. 38. 1043-1052 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kamisugi Y.: "Visualization of the Brassica self-incompatibility S-locus on identified oilseed rape chromosomes"Plant. Mol. Biol.. 38. 1081-1087 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sakamoto K.: "Characterization ; Genome sizes and morphology of sex chromosomes in hemp (Cannabis sativa L.)"Cytologia. 63. 459-464 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 神山康夫: "植物の生殖と遺伝的多様性(印刷中)"東北大学遺伝生態研究センター. (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 乗岡茂巳: "ニホンナシ自家不和合性とリボヌクレアーゼ -雄しべ・花粉間の自他認識-"蛋白質工学基礎研究センターだより. 7 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 福井希一: "染色体・ゲノム解析機器における最近の進歩 クロモソーム"ユニバーサルアカデミープレス. 179 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 福井希一: "植物染色体:構造とその機能的意義 クロモソーム"ユニバーサルアカデミープレス. 179 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fukui K.: "Frontier Researches in Plant Biotechnology"Organizing Committee of Korea-Japan Symposium on Plant Biotechnology. 120 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Wako T.: "Frontier Researches in Plant Biotechnology"Dynamics of histone acetylation during a cell cycle in barley. 120 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 北野英己: "発生分化と形態形成に関わる突然変異体 根の事典"根の事典編集委員会編 朝倉書店. 3 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nagato Y.: "Morphogenetic mutants of rice. Molecular Biology of Rice"Springer-Verlag. 21 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M. Iwano et al.: "Visualization of a self-incompatibility related SLG gene in Brassica campestris L. by multi-color FISH"Theor. Appl Genet. 96. 751-757 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] T. Entani et al.: "Centrimeric localization of an S-RNase gene in Petunia hybrida Vilm"Theor. Appl Genet. 99. 391-397 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K. Hatakeyama et al.: "Dominance relationship between S-alleles in self-incompatible Brassica campestris L"Heredity. 79. 241-247 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K. Toriyama et al.: "Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa"FEBS Lett. 424. 234-238 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K. Hatakeyama et al.: "Molecular characterization of S-locus genes, SLG and SRK, in pollen-recessive self-incompatibility haplotype of Brassica rapa L"Genetics. 149. 1587-1597 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Y. Sasaki et al.: "Localization of an SLG protein expressed under the regulation of a tapetum-specific promoter in anthers of transgenic Brassica napus"Sex. Plant Reprod. 11. 245-250 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Y. Tachikawa et al.: "Changes in protein pattern during stress-induction of carrot (Daucus carota L.) somatic embryogenesis"Plant Biotechnology. 15. 17-22 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K. Higashi et al.: "Patterns of expression of glutamine synthase isoformes during somatic and zygotic embryogenesis in carrot"Plant Cell Physiol. 39. 418-424 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K. Sage-Ono et al.: "Molecular identification of two genes of germin-like protein in Arabidopsis"Plant Biotechnology. 15. 103-108 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K. Sage-Ono et al.: "Accumulation of a clock-regulated transcript during flower-inductive darkness in Pharbitis"Plant Physiol. 116. 1479-1485 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K. Sage-Ono et al.: "Dark inducible accumulation of mRNA for a transcriptionally controlled tumor protein (TCTP) homolog in Pharbitis nil"Plant Cell Physiol. 39. 357-360 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K. Sakamoto et al.: "Characterization; Genome sizes and morphology of sex chromosomes in hemp (Canabis sativa L.)"Cytologia. 63. 459-464 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Escalante, A et al.: "RFLP analysis and genomic in situ hybridization (GISH) in somatic hybrids and their progeny between Lycopersicon esculentum and Solanum lycopersicoides"Theor. Appl. Genent. 96. 719-726 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Fukui K, et al.: "Quantitative karyotyping of three diploid Brassica species by imaging methods and localization of 45S rDNA loci on the identified chromosomes"Theor. Appl. Genent. 96. 325-330 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Mizukami Y. et al.: "Agronomic and cytological characterization of F1 hybrids between Lolium multiflorum Lam. And Festuca arundinacea var. glaucescens Boiss"Grassland. 44. 14-21 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Mitsunaga S. et al.: "Detection of proteins binding to the promoter regions DNA using a nonradioactive gel-retardation assay"Biosci. Biotecnol. Biochem. 62. 1612-1814 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Rajyashri K.R. et al.: "Isolation and FISH mapping of Yeast Artificial Chromosome (YACs) encompassing an allele of the Gm2 gene for gall midge resistance in rice"Theor. Appl. Genent. 97. 507-514 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Wako T. et al.: "Comparative analysis of the topographical distribution of acetylated histone H4 using confocal microscope and deconvolution system"Anal. Chim. Acta. 365. 9-17 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K. Hatakeyama et al.: "High sequence similarity between SLG and the receptor domain of SRK is not necessarily involved in higher dominance relationships in stigma in self-incompatible Brassica rapa L"Sex. Plant Reprod. 11. 292-294 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] M. Watanabe et al.: "Sequence comparison of four SLR1 alleles in Brassica campestris (syn. rapa)"Sex. Plant Reprod. 11. 295-296 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K. Sasaki et al.: "Detection of glycoproteins in carrot somatic embryos induced by 2,4-D or stress treatment"Plant Biotechnology. 15. 241-243 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K. Higashi et al.: "Inhibitory conditioning for carrot somatic embryogenesis in higher cell density cultures"Plant Cell Rep. 18. 2-6 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] H. Shiota et al.: "C-ABI3, the carrot homologue of the Arabidopsis ABI3 gene, is expressed during both zygotic and somatic embryogenesis and functions in the regulation of embryo-specific ABA-inducible genes"Plant Cell Physiol. 39. 1184-1193 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K. Kiyosue et al.: "A chromo box gene from carrot) Daucuc carota L.) : its cDNA structure and expression during somatic and zygotic embryogeneisi"BBA. 1398. 42-46 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamagishi M. et al.: "Chromosomal regions controlling anther culturability in rice (Oryza sativa L.)"Euphytica. 103. 227-234 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kato et al.: "Condensation pattern analysis using a newly developed chromosome image analysing system (CHIAS III) Chromosome Res"Chromosome Res. 6. 473-479 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shishido R. et al.: "Detection of specific chromosome reduction in rice somatic hybrids with the A, B, and C genomes by multi-color genomic in situ hybridization"Theor. Appl. Genent. 97. 1013-1018 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ohmido N. et al.: "Physical mapping of unique nucleotide sequences on identified rice chromosomes"Plant Mol. Biol. 38. 1043-1052 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kamisugi Y. et al.: "Visualization of the Brassica self-incompatibility S-locus on identified oilseed rape chromosomes"Plant Mol. Biol. 38. 1081-1087 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sakamoto K. Characterization: "Genome sizes and morphology of sex chromosomes in hemp (Canabis sativaL.)"Cytologia. 63. 459-464 (1998)
  • Description: 「研究成果報告書概要(欧文)」より