1997 Fiscal Year Final Research Report Summary
Study on the mechanism of silkworm resistancea against diseases.
| Project/Area Number |
07306004
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
蚕糸・昆虫利用学
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
HUKUHARA Toshiko Tokyo University of Agriculture and Technology, Faculty of Agriculture, Professor, 農学部, 教授 (70011880)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Michihiro Nagoya University Faculty of Agriculture, Professor, 農学部, 教授 (60111837)
SIMIZU Susumu Kyoto University of Textile, Faculty of Textile, Professor, 繊維学部, 教授 (20187454)
SEKIJIMA Yasutaka Saitama Junior College of Hygiene, 教授 (00104985)
IIZUKA Toshihiko Hokkaido University, Faculty of Agriculture, Professor, 農学部, 教授 (50001441)
KAWARABATA Isamu Kyushu University, Faculty of Agriculture, Professor, 農学部, 教授 (60038221)
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| Project Period (FY) |
1995 – 1997
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| Keywords | entomopoxvirus / persitent infection / microsporidia / Bacillus thuringiensis / Paecilomyces fumoso-roseus / unclear polyhedrosis virus / Bombyx mori / Pseudaletia separata |
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| Research Abstract |
A Pseudaletia separata entomopoxvirus gene coding for a virus enhancing factor was cloned and sequaenced. It was highly homologous with fusolin genes. The factor promoted the fusion of the envelope of a nuclear polyhedrosis virus with the cell plasma membrane of an insect cell line that had been derived from the hemocyte of P.separata. When several establilshed lepidopteran cell lines that had been infected with microsporidia were passaged, persistantly infected cultures were obtained due to the production, by the infected cells, of a factor inhibitory for the growth of the sporoplasm. A strain of Bacillus thuringiensis from Indonesian soil produced three morphologically different toxic crystals. The toxic polypeptides were 130 and 65 kDa in molecular mass and caised death of lepidopteran insects. A factor which inhibited C3b activation in the alternate pathway of the human complement system was isolated from silkworm hemolymph. An entomogenous fungus, Paecilomyces fumoso-roseus, produced a protease which might play an important role in invasion via the integument. The midgut epithelium of the silkworm produced 45.5 and 44 kDa polypeptides when infected with a densonucleosis virus(type2). They appearaed to be a secondary product associated with cellular death because they were not coded by the virus genome.
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