1996 Fiscal Year Final Research Report Summary
Studies on the structure and functions of cellulase
| Project/Area Number |
07306005
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 総合 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
ARAI Motoo Osaka Prefecture University, Dept, Agriculture, Professor, 農学部, 教授 (80081537)
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| Co-Investigator(Kenkyū-buntansha) |
KANDA Takahisa Shinshu University, Dept.Technology, Professor, 工学部, 教授 (60020997)
MORIKARA Yasusi Nagaoka Institure of Technology, Dept.technology, Professor, 工学部, 教授 (50239638)
OHMIYA Kunio Mie University, Dept, Agriculture, Professor, 生物資源学部, 教授 (60023488)
TUKAGOSHI Norihiro Nagoya University Dept, Agriculture, Professor, 農学部, 教授 (50115599)
OKADA Gentarou Sizuoka University, Dept.Education, Professor, 教育学部, 教授 (70021904)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Cellulase / Glucanase / Glucosidase / Gene / Cloning / DNA / hydrogen gas |
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| Research Abstract |
The results were as follows :
(1) Avicelase gene of A.aculeatus was cloned and elucidated its nucleotide sequence. The gene was expressed in yeast. (ARAI)
(2) Subsite of beta-glucosidase of A.niger recognized glucose strictly and this may reflect substrate specificity. (OKADA)
(3) Two endoglucanase genes of A.oryzae were cloned and its nucleotide sequences were elucidated.
(4) Cellulase gene (CelE) of Clostridium thermocellum was cloned and elucidated its nucleotide sequence. The gene was expressed in Escherichia coli. (OHMIYA)
(5) Endoglucanase gene of T.reesei was cloned and elucidated its nucleotide sequence. The gene was expressed. (MORIKAWA)
(6) Substrate specificities of 3 endoglucanases from fungi. Enzymatic hydrolysis mechanism depended on the structure of substrate. (KANDA)
(7) Highly expressed cDNA at stationary phase of tabacco culture cell was searched and that DNA was isolated. (SHINMYO)
(8) Celloanhydrooligosaccharides were useful for elucidation of reaction mechanism of cellulases because of marking effect of anhydroring. (ADUMA)
(9) Rhodobacter capsulatus produced effectively hydrogen gas from cellobiose. (OHSHIMA)
(10) Three cellulases from A.niger were purified and there characteristics were elucidated. (OGAWA)
(11) Three type of chimeric enzymes were prepared from glucosidases from Celluvibrio gilvus (CG) and Agrobacterium tumefaciens (AT). There properties were studied. (HAYASHI)
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