1997 Fiscal Year Final Research Report Summary
STUDIES ON THE EPIDEMIOLOGY AND DIAGNOSIS OF Q FEVER
| Project/Area Number |
07306015
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | GIFU UNIVERSITY |
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Principal Investigator |
HIRAI Katsuya Gifu University, Department of Agriculture, Professor, 農学部, 教授 (30021702)
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| Co-Investigator(Kenkyū-buntansha) |
TSUBOI Yoshimasa National Institute of Infectious Diseases, Chief Researcher, 感染症疫学部, 主任研究官 (20227466)
AMANO Ken'Ichi Akita university, School of Medicine, Associate Professor, 医学部, 助教授 (40113766)
AKIYAMA Masato Shizuoka Prefecture, Institute of Public Health & Environmental Science, Chief R, 微生物部, (研究職)主幹
MORITA Chiharu Rakunogakuen University, Department of Agriculture, Professor, 獣医学部, 教授 (50072369)
ODA Hiroshi Kagoshima University, School of medicine, Professor, 医学部, 教授 (40107868)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Q fever / Coxiellosis / Epidemiology / Zoonosis / Diagnosis / Acute and chronic Q fever |
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| Research Abstract |
The serological and bacteriological evidences of Coxiella burnetii infection in humans and animals have shown that Q fever is widespread in Japan, and suggest that domestic animals are the important reservoirs of C.burnetii. It is likely that the inhalation of contaminated aerosols is the most important route by which C.burnetii infects animals and humans in Japan. The Japanese isolates of C.burnetii are associated with an acute form of Q fever. These isolates had various degrees of virulence (high, moderate and low), but are strikingly similar to one another and to reference strains of acute infection with respect to the structure and immunogenicity of proteins and LPSs, and the nucleotide sequences of 16S rRNA,com1, Cbmip and qrsA genes. We developed a rapid method for detection of C.burnetii antibodies using high-density particle agglutination, and another for detection of C.burnetii DNA by using nested polymerase chain reaction.
We have obtained much knowledges of the epidemiology of Q fever along with some characteristics of C.burnetii isolates in Japan, which could help to set up active surveillance programs to study the epidemiology and prevention of the disease.
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[Publications] To, H., Htwe, K.K., Yamasaki, N., Zhang, G.Q., Ogawa, M., Yamaguchi, T., Fukushi, H., and Hirai, K.: "Isolation of Coxiella burnetii from dairy cattle and ticks, and some characteristics of isolates in Japan" Microbiol.Immunol.39. 663-671 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] To, H., Kako, N., Zhang, G.Q., Otuka, H., Ogawa, M., Ochiai, O., Nguyen, S.V., Yamaguchi, T., Fukushi, H., Nagaoka, H., Akiyama, M., Amano, K., and Hirai, K.: "Q fever pneumonia in children in Japan" J.Clin.Microbiol.34. 647-651 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Nguyen, S.V., Otsuka, H., Zhang, G.O., To, H., Yamaguchi, T., Fukushi, H., and Hirai, K.: "Rapid method for detection of Coxiella burnetii antibodies using high-density particle agglutination" J.Clin.Microbiol.34. 2947-2951 (1966)
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「研究成果報告書概要(欧文)」より
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[Publications] Zhang, G.Q., To, H., Nguyen, S.V., Ogawa, M., Hotta, A., Yamaguchi, T., Fukushi, H., and Hirai, K.: "Clinical evaluation of new polymerase chain reaction assay for detection of Coxiella burnetii in human serum samples." J.Clin.Microbiol.36. 77-80 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] To, H., Hotta, A., Zhang, G.Q., Nguyen, S.V., Ogawa, M., Amano, K., Yamaguchi, T., Fukushi, H., and Hirai, K.: "Antigenic characteristics polypeptides of Coxiella burnetii isolates" Microbiol.Immunol.42. 81-85 (1998)
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