1995 Fiscal Year Annual Research Report

挿入突然変異体による粘菌細胞の分化と形態形成の解析

Research Project

Project/Area Number	07308052
Research Category	Grant-in-Aid for Co-operative Research (A)
Research Institution	Okazaki National Research Institutes
Principal Investigator	竹内郁夫岡崎国立共同研究機構, 機構長 (90025239)
Co-Investigator(Kenkyū-buntansha)	田仲可昌筑波大学, 生物科学系, 教授 (80091908) 前田ミネ子大阪大学, 理学部, 助手 (70029700) 前田靖男東北大学, 大学院・理学研究科, 教授 (50025417) 落合廣北海道大学, 大学院・理学研究科, 教授 (10002122) 須藤和夫東京大学, 教養学部, 助教授 (20111453)
Keywords	細胞性粘菌 / 挿入突然変異体 / 遺伝子工学 / 形態形成 / 発生 / 細胞分化 / Dictyostelium / シグナル伝達
Research Abstract	竹内は粘菌のゲノム・プロジェクトを推進するワーキンググループを研究班内に組織し、具体的計画の作製に努め、イギリス・アメリカのグループとの国際的対応もはかりつつある。形態形成の分子機構須藤は挿入突然変異法により細胞質分裂変異株と走化性変異株を単離した。その一つはRas GTPase activating protein領域を含む遺伝子であった。落合は接着タンパク質gp64にたいするanti-sense RNA法により、遺伝子発現を抑制すると細胞接着能が減少する事を確認した。祐村は細胞骨格関連タンパク質であるAB-120とAB-95を欠損させた細胞の細胞骨格の構築を検討した。細胞分化の分子機構前田(靖)は増殖・分化の切り換え過程に発現する遺伝子を見出し、一つは新規のカルシュウム結合性タンパク質であり、また、分化の比率調節にシアン耐性呼吸が重要である事を明らかにした。大畠は予定胞子細胞の分化誘導因子を単一に精製した。石田は多細胞体の形成後、乳頭突起形成期で発生が停止するtipless挿入突然変異体を分離し、この遺伝子の一部を分離した。前田(ミ)はMAPキナーゼ(ERK2)活性化経路を解析した。ERK2は細胞外のcAMPにより活性化され、この活性化は細胞膜上のcAMPの受容体への結合を介して起こった。小山は高浸透圧ストレスの細胞内情報伝達にたいする作用を調べ、Na/H交換体の関与を示唆する結果を得た。田仲はPolysphondylium属に挿入突然変異法を適用し複数個の変異体を得た。漆原は有性生殖異常の挿入変異体を分離した。

Research Products
(34 results)

All Other

All Publications (34 results)

[Publications] Takeuchi,I.: "Regulation of Cell differentiation and pattern formation in Dictyostleilium devleopment," Int.J.Dev.Biol.38. 311-319 (1994)
[Publications] Nakao,H.: "Dictyostelium prespore specific gene Dp87 encodes a sorus matrix protein." J.Cell Sci.107. 397-403 (1994)
[Publications] Traynor,D.: "Aberrant pattern formation in myosin heavy chain mutants of Dictyostelium.21GC03:Development" 120. 591-601 (1994)
[Publications] Morio,T.: "Cooperation of positively and negatively acting promoter elements determines prespore-specific transcription of Dp87 gene in Dictyostelium." Mech.Dev.45. 59-72 (1994)
[Publications] Araki,T.: "Cell-cycle-dependent sorting in the development of Dictyostelium cells." Dev.Biol.162. 221-228 (1994)
[Publications] Fisher,A.J.: "Structures of Beryllium and Aluminium metallofluoride-Mg-ADP complexes of the Dictyostelium discoideum myosin motor domain." Biochemistry. 34. 8960-8972 (1995)
[Publications] Fisher,A.J.: "The preli0,,,minary structure of the ATP-bound atate of myosin." Biophys.J.68. 19s-28s (1995)
[Publications] Aizawa,H.: "Identification,characterization,and intracellular distribution of cofilin in Dictyostelium discoideum." J.Biol.chem.270. 10923-10932 (1995)
[Publications] Adachi,H.,T.: "Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme mediated integration of the balsRicidin S resistance marker." Biophys.Biochem.Res.commun.205. 1808-1814 (1994)
[Publications] H.Ochiai.: "Effects of tunicamycin on the development of Dictyostelium discoideum," Anim.Biol.4. 3-11 (1995)
[Publications] H.Ochiai.: "Dimer formation of a putative cell-cell adhesion protein,gp64 of the cellular slime mold.polysphondylium pallidum,21GC11:Plant Cell Physiol" 37(in press). (1996)
[Publications] S.Funamoto: "Antisense RNA inactivation of gene expression of a cell-cell adhesion protein(gp64)in the cellular slime mold polysphondylium pallidum." J.Cell Sci.(in press). (1996)
[Publications] T.Saito: "Identification of a novel all-cis-5,9,12-heptadecatrienoic acid in the cellular slime mold Polysphondylium pallidum." Lipids. (in press). (1996)
[Publications] N.Nakata: "Inhibitory effects of tunicamycin on cell-cell adhesion and cell reaggregation of the cellular slime mold.polysphondylium pallidum." J.Plant Res.(in press). (1996)
[Publications] Yumura,S.: "Introduction of macromolecules into living Dictyostelium cells by electroporation." Cell Struct.Funct.20. 185-190 (1995)
[Publications] Okazaki,K.: "Differential association of three actin-bundling proteins with microfilaments in Dictyostelium amoebae." Eur.J.Cell Biol.99. 75-81 (1995)
[Publications] Iijima,N.: "A proteinous factor mediating intercellular communication during the transition of Dictyostelium cells from growth to differentiation." Zool.Sci.12. 61-69
[Publications] Abe,F.: "Specific expression of a gene encoding a novel calcium-binding protein,CAF-1,during transition of Dictyostelium cells from growth to differentiation." Develop.Growth Differ.37. 39-48
[Publications] Araki,T.: "Cell-cycle progression during the development of Dictyostelium discoideum and its relation to the subsequent cell-sorting in multicellular structures." Develop.Growth Differ.37. 479-485 (1995)
[Publications] Matsuyama,S.: "Involvement of cyanide-resistant respiration in cell-type proportioning during the Dictyostelium development." Develop.Biol.172. 182-191 (1995)
[Publications] Amagai,A.: "Dictyostelium mucoroides cells exhibit cell-cycle dependent sorting behavior as observed in D.discoideum development." Develop.Growth Differ.38. 33-40 (1995)
[Publications] Kuwayama,H.: "Aberrant cGMP-binding activity in non-chemotactic Dictyostelium discoideum mutants." Biochim.Biophy.Acta.1268. 214-220 (1995)
[Publications] Oyama,M.: "cGMP accumulation induced by hypertonic stress in Dictyostelium discoideum." J.Biol.Chem.(in press). (1996)
[Publications] Maeda,M.: "Seven Helix Chemoattractant Receptors transiently Stimulate MAP kinase in Dictyostelium:Role of heterotrimeric G-proteins" J.Biol.Chem.(in press). (1996)
[Publications] Angata,K.: "Codon usage,genetic code and phylogeny of Dictyostelium discoideum mitochondrial DNA as deduced from a 7.3-kb region." Curr.Genet.27. 249-256 (1995)
[Publications] Angata,K.: "A group-I intron in the mitochondrial large-subunit ribosomal RNA-encoding gene of Dictyostelium discoideum:same site localization in alga and in vitro self-splicing." Gene. 153. 49-55 (1995)
[Publications] Morio,T.: "Brs-REMI:An improved method for gene tagging using a new vector in Dictyostelium" J.Plant Res.108. 111-114 (1995)
[Publications] Asahi,K.: "DIF-1,morhogen of Dictyostelium discoideum,induces the erythroid differentiation in murine and human leukemia cells." Biochem.Biophys.Res.Comm.208. 1036-1039 (1995)
[Publications] Iwamoto,M.: "Mitochondrial ribosomal protein L11 gene of Dictyostelium discoidem resides not in the nuclear genome but in the mitochondrial genome." DNA Res.2. 129-132 (1995)
[Publications] Pi,M.: "Analysis of a tRNA gene-like sequence(t-element)with TTA at the anticodon position in the mitochondrial DNA of Dictyostelium discoideum." J.Plant Res.(in press). (1996)
[Publications] Ymaguchi,N.: "Targeted disruption of genes for gp138,a cell-fusion-related protein in Dictyostelium discoideum.revealed the existence of a third gene." Develop.Growth and Differ.(in press). (1996)
[Publications] Urushihara,H.: "A protease sensitive component(s)on the cell surface prevents self-fusion in a bisexual strain of Dictyostelium discoideum." Cell Structure and Function.(in Press). (1996)
[Publications] Higuchi,I.: "Self-and non-self-recognition in bisexual mating of Dictyostelium discoideum." Develop.Growth&Differ.37. 311-317 (1995)
[Publications] Fang,H.: "Antisense RNA inactivation of gp138 gene expression results in repression of sexual cell fusion in Dictyostelium discoideum." Jour.Cell.Sci.106. 785-788 (1993)

1995 Fiscal Year Annual Research Report

挿入突然変異体による粘菌細胞の分化と形態形成の解析

Principal Investigator

竹内 郁夫 岡崎国立共同研究機構, 機構長 (90025239)

Research Products

[Publications] Takeuchi,I.: "Regulation of Cell differentiation and pattern formation in Dictyostleilium devleopment," Int.J.Dev.Biol.38. 311-319 (1994)

[Publications] Nakao,H.: "Dictyostelium prespore specific gene Dp87 encodes a sorus matrix protein." J.Cell Sci.107. 397-403 (1994)

[Publications] Traynor,D.: "Aberrant pattern formation in myosin heavy chain mutants of Dictyostelium.21GC03:Development" 120. 591-601 (1994)

[Publications] Morio,T.: "Cooperation of positively and negatively acting promoter elements determines prespore-specific transcription of Dp87 gene in Dictyostelium." Mech.Dev.45. 59-72 (1994)

[Publications] Araki,T.: "Cell-cycle-dependent sorting in the development of Dictyostelium cells." Dev.Biol.162. 221-228 (1994)

[Publications] Fisher,A.J.: "Structures of Beryllium and Aluminium metallofluoride-Mg-ADP complexes of the Dictyostelium discoideum myosin motor domain." Biochemistry. 34. 8960-8972 (1995)

[Publications] Fisher,A.J.: "The preli0,,,minary structure of the ATP-bound atate of myosin." Biophys.J.68. 19s-28s (1995)

[Publications] Aizawa,H.: "Identification,characterization,and intracellular distribution of cofilin in Dictyostelium discoideum." J.Biol.chem.270. 10923-10932 (1995)

[Publications] Adachi,H.,T.: "Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme mediated integration of the balsRicidin S resistance marker." Biophys.Biochem.Res.commun.205. 1808-1814 (1994)

[Publications] H.Ochiai.: "Effects of tunicamycin on the development of Dictyostelium discoideum," Anim.Biol.4. 3-11 (1995)

[Publications] H.Ochiai.: "Dimer formation of a putative cell-cell adhesion protein,gp64 of the cellular slime mold.polysphondylium pallidum,21GC11:Plant Cell Physiol" 37(in press). (1996)

[Publications] S.Funamoto: "Antisense RNA inactivation of gene expression of a cell-cell adhesion protein(gp64)in the cellular slime mold polysphondylium pallidum." J.Cell Sci.(in press). (1996)

[Publications] T.Saito: "Identification of a novel all-cis-5,9,12-heptadecatrienoic acid in the cellular slime mold Polysphondylium pallidum." Lipids. (in press). (1996)

[Publications] N.Nakata: "Inhibitory effects of tunicamycin on cell-cell adhesion and cell reaggregation of the cellular slime mold.polysphondylium pallidum." J.Plant Res.(in press). (1996)

[Publications] Yumura,S.: "Introduction of macromolecules into living Dictyostelium cells by electroporation." Cell Struct.Funct.20. 185-190 (1995)

[Publications] Okazaki,K.: "Differential association of three actin-bundling proteins with microfilaments in Dictyostelium amoebae." Eur.J.Cell Biol.99. 75-81 (1995)

[Publications] Iijima,N.: "A proteinous factor mediating intercellular communication during the transition of Dictyostelium cells from growth to differentiation." Zool.Sci.12. 61-69

[Publications] Abe,F.: "Specific expression of a gene encoding a novel calcium-binding protein,CAF-1,during transition of Dictyostelium cells from growth to differentiation." Develop.Growth Differ.37. 39-48

[Publications] Araki,T.: "Cell-cycle progression during the development of Dictyostelium discoideum and its relation to the subsequent cell-sorting in multicellular structures." Develop.Growth Differ.37. 479-485 (1995)

[Publications] Matsuyama,S.: "Involvement of cyanide-resistant respiration in cell-type proportioning during the Dictyostelium development." Develop.Biol.172. 182-191 (1995)

[Publications] Amagai,A.: "Dictyostelium mucoroides cells exhibit cell-cycle dependent sorting behavior as observed in D.discoideum development." Develop.Growth Differ.38. 33-40 (1995)

[Publications] Kuwayama,H.: "Aberrant cGMP-binding activity in non-chemotactic Dictyostelium discoideum mutants." Biochim.Biophy.Acta.1268. 214-220 (1995)

[Publications] Oyama,M.: "cGMP accumulation induced by hypertonic stress in Dictyostelium discoideum." J.Biol.Chem.(in press). (1996)

[Publications] Maeda,M.: "Seven Helix Chemoattractant Receptors transiently Stimulate MAP kinase in Dictyostelium:Role of heterotrimeric G-proteins" J.Biol.Chem.(in press). (1996)

[Publications] Angata,K.: "Codon usage,genetic code and phylogeny of Dictyostelium discoideum mitochondrial DNA as deduced from a 7.3-kb region." Curr.Genet.27. 249-256 (1995)

[Publications] Angata,K.: "A group-I intron in the mitochondrial large-subunit ribosomal RNA-encoding gene of Dictyostelium discoideum:same site localization in alga and in vitro self-splicing." Gene. 153. 49-55 (1995)

[Publications] Morio,T.: "Brs-REMI:An improved method for gene tagging using a new vector in Dictyostelium" J.Plant Res.108. 111-114 (1995)

[Publications] Asahi,K.: "DIF-1,morhogen of Dictyostelium discoideum,induces the erythroid differentiation in murine and human leukemia cells." Biochem.Biophys.Res.Comm.208. 1036-1039 (1995)

[Publications] Iwamoto,M.: "Mitochondrial ribosomal protein L11 gene of Dictyostelium discoidem resides not in the nuclear genome but in the mitochondrial genome." DNA Res.2. 129-132 (1995)

[Publications] Pi,M.: "Analysis of a tRNA gene-like sequence(t-element)with TTA at the anticodon position in the mitochondrial DNA of Dictyostelium discoideum." J.Plant Res.(in press). (1996)

[Publications] Ymaguchi,N.: "Targeted disruption of genes for gp138,a cell-fusion-related protein in Dictyostelium discoideum.revealed the existence of a third gene." Develop.Growth and Differ.(in press). (1996)

[Publications] Urushihara,H.: "A protease sensitive component(s)on the cell surface prevents self-fusion in a bisexual strain of Dictyostelium discoideum." Cell Structure and Function.(in Press). (1996)

[Publications] Higuchi,I.: "Self-and non-self-recognition in bisexual mating of Dictyostelium discoideum." Develop.Growth&Differ.37. 311-317 (1995)

[Publications] Fang,H.: "Antisense RNA inactivation of gp138 gene expression results in repression of sexual cell fusion in Dictyostelium discoideum." Jour.Cell.Sci.106. 785-788 (1993)

竹内郁夫岡崎国立共同研究機構, 機構長 (90025239)