• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1996 Fiscal Year Final Research Report Summary

Studies of quality control of proteins in the endoplasmic reticulum.

Research Project

Project/Area Number 07308068
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section総合
Research Field Structural biochemistry
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KITO Makoto  Kyoto Univ.Research Institute for Food Science, Professor, 食糧科学研究所, 教授 (60027183)

Co-Investigator(Kenkyū-buntansha) SATO Ryuichiro  Osaka Univ.Pbarmacology, Associate Professor, 薬学部, 助教授 (50187259)
NAKANO Akihiko  Univ.of Tokyo, Science, Associate Professor, 理学部, 助教授 (90142140)
KOHNO Kenji  Nara Institute of Science and Technology, Education center for genetics, Profess, 遺伝子教育センター, 教授 (50142005)
IKEHARA Yukio  Fukuoka Univ.Medicine, Professor, 医学部, 教授 (70037612)
KIKUCHI Masakazu  Ritsumeikan Univ, Science and Technology, Professor, 理工学部, 教授 (10278492)
Project Period (FY) 1995 – 1996
Keywordsendoplasmic reticulum / quality control / protein degradation / chaperone
Research Abstract

In order to elucidate molecular mechanisms of the quality control of proteins in the endoplasmic reticulum (ER), a research team was organized. Major research results were :
the relationship between hydrophobicity of signal peptides and efficiency of translocation of ER membrane was found (Tagaya), function of PDI as a chaperone was determined and PDI related proteins were cloned in yeast (Kikuchi), involvement of calnexin, Bip and proteasome was found in retention and degradation of mutant secretory proteins (Ikehara), recombinant ER-60 protease was expressed and its active residue and ER retention signal were detemined (Kito, Urade), intracellular localization of microsomal triglyceride transfer protein was determined and its interaction with PDI was observed (Sato), cis element was identified for transcriptional control of Bip by Ssalp (Kohno), supression of expression and transportation of procollagen was shown by HSP47 antisense RNA (Hosokawa), involvement of NLS peptide and casein kinase 2 in phosphorylation of GRP94 was found (Miyata), singal for ER retention of Sec12p was determined and funtion of Rerlp was analyzed (Nakano), increased lysophosphatidylethanolamine upon membrane fusion in the regulated exocytosis was identified (Fukuoka).
A reseach meeting was organized and the research results were published (Kito).

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Hayano, T. Kikuchi, M: "Molecular cloning of the CDNA encoding a novel protein disulfide isomerase-related protein (PDIR)" FEBS Lett.,. 372. 210-214 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oda, K : Ikehara, Y et. al.: "Lactacystin, an inhibitor of the proteasome, blocks the degradation of a mutont precursor of glycosyl phosphatidyl inositol-linked protein.." Biochem, Biophys. Res. Commun. 219. 800-805 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otsu, M. Urade. R, Kito M et. al.: "A possible Role of ER-60 protease in the degradation of Misfolded protein in the endo plosmic reficulum" J. Biol. Chem.270. 14958-14961 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mayata. Y, Yahara. I.: "Interaction between Casein kinase II and the 90-KDa Stress protein HSP90." Biochemistry. 34. 8123-8129 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuge. O. Saito. K et. al.: "Post tronslotional processing of the phosphaticlyl serine decarboxylase gene product in chinese hamster Ovary cells." Biochem. J.319. 33-38 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato, M. Sato K. Natano A: "Endoplasmic reticulum localization of Sec12p is achieved by two mechanism." J. Cell Biol. 134. 279-293 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto, K. Ikehara Y et. al.: "Intracellular Protein Catabolism" Plenum Press, 336 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagata. K. Satoh. M et al.: "Molecular Chaperones in Protein(印刷中)" Academic Press, (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayano, T.Kikuchi, M.: "Molecular cloning of the cDNA encoding a novel protein disulfide isomerase-relatedprotein (PDIR)." FEBS lett.372. 210-214 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oda, K., Ikehara, Y.and Omura, S.: "Lactacystin, an inhibitor of the proteasome, blocks the degradation of a mutant precursor of glycosylphosphatidylinositol-linked protein in a Pre-Golgi compartment" Biochem.Biophys.Res.Commun.219. 800-805 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mieko Otsu, Reiko Urade, Makoto Kito, Fumihiko Omura and Masakazu Kikuchi: "A Possible Role of ER-60 Protease in the Degradation of Misfolded Proteins in the Endoplasmic Reticulum" Journal of Biological Chemistry. 270. 14958-14961 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyata, Y., and Yahara, I.: "Interaction between Casein Kinase II and the 90-kDa Stress Protein, HSP90." Bioche・istry.34. 8123-8129 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuge, O., Saito, K., Kojima, M., Akamatsu, Y., and Nishijima, M.: "Post-translational processing of the phosphatidylserine decarboxylase gene product in Chinese hamster ovary cells" Biochem.J.319. 33-38 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyuki Sato, Ken Sato, and Akihiko Nakano.: "Endoplasm ic reticulum localization of Sec12p is achieved by two mechanisms : Rerlp-dependent retrieval that requires the transmembrane domain and Rerlp-independent retention that involves the cytoplasmic domain." J.Cell Biol.134. 279-293 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, K., Kadowaki, T., Okamoto, K., Yoneda, M., Nakayama, K., Misumi, Y.and Ikehara, Y. :: Structure and function of a novel arginine-specific cysteine proteinase (argingipain) as a major periodontal pathogenic factor from Porphyromonas gingivalis. In : Intracellular Protein Catablism (Suzuki, K.and Bond, J., eds.). Plenum Press, 336 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagata K,Satoh M,Miller A,Hosokawa N: Involvement of HSP47 in the folding and processing of procollagen in the ER.in Molecular Chaperones in Protein ; Structure, Function and Mode of Action. (Eds.Fink and Goto). (In press.),

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi