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1997 Fiscal Year Final Research Report Summary

ELUCIDATION OF LIGNIFICATION MECHANISM OF WOODY PLANTS BY GENETIC ENGINEERING

Research Project

Project/Area Number 07406008
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionTOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY

Principal Investigator

MOROHOSHI Noriyuki  GRADUATE SCHOOL OF BIO-APPLICATION AND SYSTEMS ENGINEERING,DEPARTMENT OF ENVIRONMENT SYMBIOTIC PRODUCTION SYSTEM,TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY PROFESSOR, 大学院・生物システム応用科学研究科, 教授 (30015078)

Co-Investigator(Kenkyū-buntansha) KAWAI Shinya  FACULTY OF AGRICULTURE,APPLIED BIOLOGICAL SCIENCE,TOKYO UNIVERSITY OF AGRICULTUR, 農学部, 助教授 (90202027)
KATAYAMA Yoshihiro  GRADUATE SCHOOL OF BIO-APPLICATION AND SYSTEMS ENGINEERING,DEPARTMENT OF ENVIRON, 大学院・生物システム応用科学研究科, 助教授 (10214339)
Project Period (FY) 1995 – 1997
KeywordsLIGNIN / LIGNIN BIOSYNTHETIC GENE / PHENYLALANINE AMMONIA-LYASE (PAL) / CINNAMATE 4-HYDROXYLASE (CA4H) / O-METHYLTRANSFERASE (OMT) / PEROXIDASE (PO) / TOBACCO / POPLAR
Research Abstract

To elucidate the lignification mechanism of woody plants from a viewpoint of molecular biology, some genes encoding phenylalanine ammonia-ly ase (PAL), cinnamate 4-hydroxylase (CA4H), O-methyltransferase (OMT) and peroxidase (PO) were isolated from the hybrid aspen (Populus kitakamiensis). Of these genes, PAL (palg2b), CH4H (cyp73a), OMT (homt1), and PO (prxA3a, prxA4a) were identified as the genes involved in lignification by the northem hybridization and the expression observation on tissue slices with reporter gene (GUS gene).
These transformants of tobacco and poplar controlling the lignin biosynthesis by antisense RNA method with those identified genes were formed. The transformants concerning OMT have lower OMT activity and lignin content than those of normal tobacco and poplar, and have a characteristic lignin chemical structure consisting of lower amount of condensed units, and more of guaiacyl unit and degradable chemical structures.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] X.Zhan et al.: "A new approach based on the leaf disk method for Agrobacterlum medlated transformation and regeneration of aspen." Plant Science. 123. 105-112 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] X.Zhan et al.: "A new approach based on the leaf disk method for Agrobacterium mediated transformation and regenetation of aspen." Plant Science. 123. 105-112 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Osakabe et al.: "Immunocytochemical localization of phenylalanine ammonia-lyase in tissues of Populus kitakamiensis." Planta. 200. 13-19 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kawai et al.: "Isolation and analysis of cinnamic acid 4-hydroxylase homologous genes from a hybrid Aspen, Populus kitakamiensis." Biosci.Biotech.Biochem.60(10). 1586-1597 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Hayakawa et al.: "Molecular cloning and tissue-specific expression of two genes that encode caffeic acid and O-methyltransferases from Populus kitakamiensis." Plant Science. 113. 157-165 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Osakabe et al.: "Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis." Plant Molecular Biology. 28. 1133-1141 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Osakabe et al.: "Molecular cloning of two tandemly arranged peroxidase genes from Populus Kitakamiensis and their differential regulation in the stem." Plant Molecular Biology. 28. 677-689 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Osakabe et al.: "Structure and tissue-specific expression of genes for phenylalanine ammonia-lyase from a hybrid aspen, Populus kitakamiensis." Plant Science. 105. 217-226 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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