1997 Fiscal Year Annual Research Report

ポルフィリン生合成阻害と葉緑体チラコイド膜の破壊

Research Project

Project/Area Number	07406017
Research Institution	Tamagawa University
Principal Investigator	若林攻玉川大学, 農学部, 教授 (00220830)
Co-Investigator(Kenkyū-buntansha)	佐藤幸治玉川大学, 農学部, 教授 (40119516) 河野均玉川大学, 農学部, 教授 (40074356) 平井憲次 (財)相模中央化学研究所, 主任研究員
Keywords	Peroxidizing作用機構 / 活性酵素 / プロトポルフィリノーゲン酸化酵素 / チラコイド膜破壊 / HPLC-イソルミノール化学発光法 / プロトポルフィリノーゲン酸化酵素阻害剤 / Diphenyl ether系化合物 / 環状イミド系化合物
Research Abstract	単細胞緑藻(Scenedesmus acutus)を用いた実験で確認された,“プロトポルフィリノーゲン-IX酸化酵素の阻害,ポロトポルフィリン-IXの蓄積,エタン発生をともなうチラコイド膜の破壊,光合成色素の減少"といういわゆるPeroxidizing植物毒性作用の解明を目的として,科学研究費交付最終年度の検討を行い,以下の成果〈[1]〜[4]〉を得た. [1]HPLC-イソルミノール化学発光を原理とする全自動脂質分析システムを用い,プロトポルフィリノーゲン-IX酸化酵素阻害剤処理後に生ずる過酸化脂質を測定し,活性酸素が関与するチラコイド膜破壊の機構を明らかにした. [2]クロロフィル生合成能を有する植物培養細胞を用いた生物試験を行い,上記のPeroxidizing植物毒性作用が緑色植物の葉緑体中で普遍的に起こることを確認した. [3]光存在下で活性酸素を発生させチラコイド膜破壊を誘導する新しいプロルフィリノーゲン酸化酵素阻害剤の分子設計と合成を行なった. [4]分子設計し合成した阻害剤の多くは,各種植物のプロトポルフィリノーゲン酸化酵素を阻害することが確認された. 平成9年度の研究で得られた結果は,(11)項記載の雑誌等に発表した.

Research Products
(11 results)

All Other

All Publications (11 results)

[Publications] Beate Nicolaus et al: "Activation of Proherbicides by Glutathinoe S-Transferase" Proceeding ; NATO Advanced Research Workshop : Regulation of Enzymatic Systems Detoxifying Xenobiotics in Plants. 32- (1996)
[Publications] Hitoshi Kohno et al: "The Effects of PET Inhibitors on Peroxidizing Phytotoxic Activity" Proceeding ; 10th Congress,European Plant Physiology and Biochemistry Society. 299-300 (1996)
[Publications] Ko Wakabayashi: "Present Status and Future Prospect of Peroxidizing Herbicides" Fine Chemicals. 26. 5-19 (1997)
[Publications] Yukiharu Sato et al: "The Enzymatic Activation of Peroxidizing Cyclic Imide : A New Function of Glutathione S-Transferese and Glutathione" J.Pesticide Science. 22. 33-36 (1997)
[Publications] Chikaaki Muro et al: "Effect of Rhodanine and 2(5H)-Thiophenone in Green Algae and Liverwort Cells" J.Pesticide Science. 22. 1-5 (1997)
[Publications] Atsushi Uchida et al: "Isomerization of 3,4-Dialkyl-1,3,4-thiadiazolidines and 3,4-Alkylene-1,3,4-thiadiazolidines by Glutathione S-Transferase" Zeitschrift fur Naturforschung. 52c. 345-350 (1997)
[Publications] Shizuka Ishida et al: "Protoporphyrinogen-IX Oxidase Inhibition by N-(2,4,5-Trisubstituted phenyl)-3,4,5,6-tetrahydrophthalimides" J.Pesticide Science. 22. 299-302 (1997)
[Publications] Tetsuji Iida et al: "Peroxidizing Phtotoxicities of 1,2-Dialkyl-1,2,4-triazolidines and 3,4-Dialkyl-1,3,4-thiadiazolidines" J.Pesticide Science. 22. 303-308 (1997)
[Publications] Aiko Ohki et al: "Phytotoxicity Caused by Peroxidizing Herbicides Is Alleviated by 2-Substituted 4,6-Bis(ethylamino)-1,3,5-triazines" J.Pesticide Science. 22. 309-313 (1997)
[Publications] Pyoichi Uraguchi et al: "Molecular Shape Similarity of Cyclic Imides and Protoporphyrinogen-IX" J.Pesticide Science. 22. 314-320 (1997)
[Publications] T.Jablonkai et al: "Chemical Catalysis of the Isomerization of a Peroxidizing Herbicidal Thiadiazolidine" Proceeding : The 1997 Brighton Crop Protection Conference-Weeds. 2. 771-776 (1997)

1997 Fiscal Year Annual Research Report

ポルフィリン生合成阻害と葉緑体チラコイド膜の破壊

Principal Investigator

若林 攻 玉川大学, 農学部, 教授 (00220830)

Research Products

[Publications] Beate Nicolaus et al: "Activation of Proherbicides by Glutathinoe S-Transferase" Proceeding ; NATO Advanced Research Workshop : Regulation of Enzymatic Systems Detoxifying Xenobiotics in Plants. 32- (1996)

[Publications] Hitoshi Kohno et al: "The Effects of PET Inhibitors on Peroxidizing Phytotoxic Activity" Proceeding ; 10th Congress,European Plant Physiology and Biochemistry Society. 299-300 (1996)

[Publications] Ko Wakabayashi: "Present Status and Future Prospect of Peroxidizing Herbicides" Fine Chemicals. 26. 5-19 (1997)

[Publications] Yukiharu Sato et al: "The Enzymatic Activation of Peroxidizing Cyclic Imide : A New Function of Glutathione S-Transferese and Glutathione" J.Pesticide Science. 22. 33-36 (1997)

[Publications] Chikaaki Muro et al: "Effect of Rhodanine and 2(5H)-Thiophenone in Green Algae and Liverwort Cells" J.Pesticide Science. 22. 1-5 (1997)

[Publications] Atsushi Uchida et al: "Isomerization of 3,4-Dialkyl-1,3,4-thiadiazolidines and 3,4-Alkylene-1,3,4-thiadiazolidines by Glutathione S-Transferase" Zeitschrift fur Naturforschung. 52c. 345-350 (1997)

[Publications] Shizuka Ishida et al: "Protoporphyrinogen-IX Oxidase Inhibition by N-(2,4,5-Trisubstituted phenyl)-3,4,5,6-tetrahydrophthalimides" J.Pesticide Science. 22. 299-302 (1997)

[Publications] Tetsuji Iida et al: "Peroxidizing Phtotoxicities of 1,2-Dialkyl-1,2,4-triazolidines and 3,4-Dialkyl-1,3,4-thiadiazolidines" J.Pesticide Science. 22. 303-308 (1997)

[Publications] Aiko Ohki et al: "Phytotoxicity Caused by Peroxidizing Herbicides Is Alleviated by 2-Substituted 4,6-Bis(ethylamino)-1,3,5-triazines" J.Pesticide Science. 22. 309-313 (1997)

[Publications] Pyoichi Uraguchi et al: "Molecular Shape Similarity of Cyclic Imides and Protoporphyrinogen-IX" J.Pesticide Science. 22. 314-320 (1997)

[Publications] T.Jablonkai et al: "Chemical Catalysis of the Isomerization of a Peroxidizing Herbicidal Thiadiazolidine" Proceeding : The 1997 Brighton Crop Protection Conference-Weeds. 2. 771-776 (1997)

若林攻玉川大学, 農学部, 教授 (00220830)