1997 Fiscal Year Final Research Report Summary
STUDY OF STR-DNA POLYMORPHISM ON HGH AND DIS53 SYSTEMS.
| Project/Area Number |
07407014
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | TOKYO MEDICAL AND DENTAL UNIVERSITY |
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Principal Investigator |
HASEKURA Hayato SCH.MED.DEPT.FORENSIC MED., PROF., 医学部, 教授 (40049789)
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| Co-Investigator(Kenkyū-buntansha) |
YONEMURA Isamu SCH.MED.DEPT.FORENSIC MED., LECTURER, 医学部, 講師 (30020762)
SATO Keita SCH.MED.DEPT.FORENSIC MED., ASSISTANT PROF., 医学部, 助手 (00280975)
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| Project Period (FY) |
1995 – 1997
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| Keywords | STR / HGH / DIS53 / Forensic genetics |
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| Research Abstract |
STR systems in general are known to have easy and reproducible detection methods with the use of allelic markers. However, some systems have fewer alleles with unbalanced frequencies and less useful in forensic practice in Japan. In this study on the HGH systems. 28 alleles (micro variants) were detected from 43 persons. The heterozygosity in Japanese was as high as 100%. So this system is expected to be highly useful in Japan in practice of personal identification and paternity testing. HGH alleles are complex gene ; it is difficult to differentiate between different subtypes in equal fragment size by electrophoresis of PCR products. This result suggested that sequence analysis is most appropriate in typing HGH polymorphisms. A problem of HGH system is that population data of this systems are difficult to obtain because of its extensive polymorphism with very low allele frequencies. However, this problem were improved by developing an allelic ladder marker, and typing by PCR-SSCP methods. By this, we can identify a heterozygote of different genes of an equal alleles size with different genes from a homozygote, and establish typing method for HGH alleles without sequencing process.
In study of D1S53, it took long times to establish of evacuation method of extra bands were raised by primer's skipping on PCR reaction. This is common problem in 2 base-STR system. We discovered new method for evacuation of extra bands by using specific polymerase in 1997.
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