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1997 Fiscal Year Final Research Report Summary

STUDY OF STR-DNA POLYMORPHISM ON HGH AND DIS53 SYSTEMS.

Research Project

Project/Area Number 07407014
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionTOKYO MEDICAL AND DENTAL UNIVERSITY

Principal Investigator

HASEKURA Hayato  SCH.MED.DEPT.FORENSIC MED., PROF., 医学部, 教授 (40049789)

Co-Investigator(Kenkyū-buntansha) YONEMURA Isamu  SCH.MED.DEPT.FORENSIC MED., LECTURER, 医学部, 講師 (30020762)
SATO Keita  SCH.MED.DEPT.FORENSIC MED., ASSISTANT PROF., 医学部, 助手 (00280975)
Project Period (FY) 1995 – 1997
KeywordsSTR / HGH / DIS53 / Forensic genetics
Research Abstract

STR systems in general are known to have easy and reproducible detection methods with the use of allelic markers. However, some systems have fewer alleles with unbalanced frequencies and less useful in forensic practice in Japan. In this study on the HGH systems. 28 alleles (micro variants) were detected from 43 persons. The heterozygosity in Japanese was as high as 100%. So this system is expected to be highly useful in Japan in practice of personal identification and paternity testing. HGH alleles are complex gene ; it is difficult to differentiate between different subtypes in equal fragment size by electrophoresis of PCR products. This result suggested that sequence analysis is most appropriate in typing HGH polymorphisms. A problem of HGH system is that population data of this systems are difficult to obtain because of its extensive polymorphism with very low allele frequencies. However, this problem were improved by developing an allelic ladder marker, and typing by PCR-SSCP methods. By this, we can identify a heterozygote of different genes of an equal alleles size with different genes from a homozygote, and establish typing method for HGH alleles without sequencing process.
In study of D1S53, it took long times to establish of evacuation method of extra bands were raised by primer's skipping on PCR reaction. This is common problem in 2 base-STR system. We discovered new method for evacuation of extra bands by using specific polymerase in 1997.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 佐藤慶太、支倉逸人他: "日本人におけるSTR-HGH多型性の検出 (第1報)" DNA多型. 4. 104-108 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤慶太、支倉逸人他: "日本人におけるSTR-HGH多型性の検出 (第2報)" DNA多型. 5. 144-147 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤慶太、支倉逸人他: "PCR-SSCP法による同鎖長異型のHGHアリル・ヘテロ接合体の検出" DNA多型. 6. (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keita Sato,et al.: "Coplex-STR Polymorphism in HGH Genes in Japanese." 日本犯罪学雑誌. 63. 164-168 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keita Sato, Hayato Haseukra, et al.: "Detection of HGH alleles in Japanese (I)" DNA POLYMORPHISM. VOL.4. 104-106 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keita Sato, Hayato Haseukra, et al.: "Detection of HGH alleles in Japanese (II)" DNA POLYMORPHISM. VOL.5. 144-147 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keita Sato, Hayato Haseukra, et al.: "PCR-SSCP detection of heterozygous HGH micro variant alleles from single bands in fragment size typing." DNA POLYMORPHISM. VOL.6. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keita Sato, Hayato Haseukra, et al.: "Complex-STR Polymorphism in HGH Genes in Japanese." ACTA CRIM.JAPONICA. 63 5-6. 164-168 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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